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AB282302

Human Polymeric immunoglobulin receptor ELISA Kit (PIGR)

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Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) is a single-wash 90-min Simplestep used to quantify Human Polymeric immunoglobulin receptor (PIGR) with a sensitivity of 29.16 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Polymeric immunoglobulin receptor, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, PIGR

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ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)
  • ELISA

Supplier Data

ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)
Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)

Interpolated concentrations of native Polymeric immunoglobulin receptor in human stomach tissue extract based on a 500 μg/mL extract load.

The concentrations of Polymeric immunoglobulin receptor were measured in duplicate and interpolated from the Polymeric immunoglobulin receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Polymeric immunoglobulin receptor concentration was determined to be 11.18 ng/mL in stomach tissue extract.

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)

Interpolated concentrations of native Polymeric immunoglobulin receptor in human serum and plasma samples.

The concentrations of Polymeric immunoglobulin receptor were measured in duplicates, interpolated from the Polymeric immunoglobulin receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1.25%, plasma (citrate) 1.25%, plasma (EDTA) 1.25% and plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Polymeric immunoglobulin receptor concentration was determined to be 613.22 ng/mL in serum, 713.68 ng/mL in plasma (citrate), 789.90 ng/mL in plasma (EDTA), and 666.13 ng/mL in plasma (heparin).

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)

Example of human Polymeric immunoglobulin receptor standard curve in Sample Diluent NS.

The Polymeric immunoglobulin receptor standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)
  • sELISA

Supplier Data

Sandwich ELISA - Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) (AB282302)

1 : 320 diluted Serum from ten individual healthy human female donors were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Polymeric immunoglobulin receptor concentration was determined to be 962.86 ng/mL with a range of 231.17 – 2272.98 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 29.16 pg/mL

Range

187.5 - 12000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) ab282302 is a rapid single-wash 90-min Sandwich ELISA to measure Human Polymeric immunoglobulin receptor (PIGR)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 29.16 pg/mL.

How the assay works

Human Polymeric immunoglobulin receptor (PIGR)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Polymeric immunoglobulin receptor ELISA Kit (PIGR) ab282302 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-46] - BSA and Azide free (Capture) ab281123
- Anti-Polymeric immunoglobulin receptor/PIGR antibody [EPR23314-243] - BSA and Azide free (Detector) ab281123

Human Polymeric immunoglobulin receptor (PIGR) mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. PIGR binds polymeric IgA and IgM at the basolateral surface of epithelial cells and the complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the PIGR extracellular (known as the secretory component) from the transmembrane segment. This kit was designed to target the extracellular region of PIGR. The extracellular region of PIGR shares 65%, 64%, 68% and, 88% sequence homology with mouse, rat, cow, and monkey, respectively.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 87 %", "average": "= 85" }, { "sample": "EDTA Plasma", "range": "80 - 87 %", "average": "= 83" }, { "sample": "Cell culture media", "range": "85 - 89 %", "average": "= 87" }, { "sample": "Heparin Plasma", "range": "81 - 92 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "81 - 98 %", "average": "= 90" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab282302-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB282302-CMP05", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB282302-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB282302-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB282302-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB282302-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Polymeric immunoglobulin receptor (PIGR) Capture Antibody", "number":"AB282302-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB282302-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Polymeric immunoglobulin receptor (PIGR) Lyophilized Recombinant Protein", "number":"AB282302-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB282302-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Polymeric immunoglobulin receptor (PIGR) Detector Antibody", "number":"AB282302-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB282302-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The polymeric immunoglobulin receptor often referred to as PIGR or the polymeric Ig receptor is a critical component in the immune system. This protein which has a mass of approximately 83 kDa is expressed primarily on the basolateral surface of epithelial cells. Common expression sites include the respiratory gastrointestinal and genital tracts. PIGR facilitates the transcytosis of polymeric immunoglobulins like IgA and IgM across epithelial cells ensuring that these antibodies reach mucosal sites where they perform functions.
Biological function summary

The polymeric immunoglobulin receptor plays an essential role in mucosal immunity by transporting IgA and IgM antibodies to mucosal surfaces. The PIGR is a part of a complex that includes the secretory component which protects immunoglobulins from proteolytic degradation. This mechanism is fundamental for safeguarding mucosal surfaces while allowing the immune system to respond swiftly to pathogens.

Pathways

The interaction of PIGR with polymeric immunoglobulins is an important event in the mucosal immune pathway. This pathway is important in maintaining mucosal homeostasis and pathogen defense. PIGR works closely with immunoglobulin proteins like IgA and IgM enabling their rapid and efficient transport across epithelial barriers. This ensures that the immune system rapidly addresses potential threats at mucosal sites.

Defects in PIGR function can lead to conditions like IgA nephropathy and selective IgA deficiency. These disorders highlight the importance of the polymeric Ig receptor in immune health and suggest its role in maintaining immunoglobulin balance. There is evidence suggesting potential connections between PIGR dysfunction and the proteins involved in immune regulation which helps in understanding how PIGR contributes to disease pathology.
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Product protocols

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Target data

Polymeric immunoglobulin receptor. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.. Secretory component. Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens (PubMed : 12150896). On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells (PubMed : 16543244).
See full target information PIGR
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websiteProtocolBooklet
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