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AB325725

Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent

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Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Polymeric immunoglobulin receptor (PIGR) with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 109.74 - 80000 pg/ml with a sensitivity of 40.88 pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Polymeric immunoglobulin receptor, PIgR, Poly-Ig receptor, Hepatocellular carcinoma-associated protein TB6, PIGR

6 Images
Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)
  • sELISA

Lab

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)

Standard curve comparison between the Chemiluminescent Human Polymeric immunoglobulin receptor SimpleStep ELISA and colorimetric Human Polymeric immunoglobulin receptor SimpleStep ELISA (ab282302) in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)
  • sELISA

Lab

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)

Interpolated concentrations of native Polymeric immunoglobulin receptor in human serum and plasma samples. The concentrations of Polymeric immunoglobulin receptor were measured in duplicates, interpolated from the Polymeric immunoglobulin receptor standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1.25%, plasma (citrate) 1.25%, plasma (EDTA) 1.25% and plasma (heparin) 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Polymeric immunoglobulin receptor concentration was determined to be 613.22 ng/mL in serum, 713.68 ng/mL in plasma (citrate), 789.90 ng/mL in plasma (EDTA), and 666.13 ng/mL in plasma (heparin).

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)
  • sELISA

Lab

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)

1 : 320 diluted Serum from ten individual healthy human female donors were measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Polymeric immunoglobulin receptor concentration was determined to be 962.86 ng/mL with a range of 231.17 – 2,272.98 ng/mL.

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)
  • sELISA

Lab

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)

Standard curve comparison between the Chemiluminescent Human Polymeric immunoglobulin receptor SimpleStep ELISA and colorimetric Human Polymeric immunoglobulin receptor SimpleStep ELISA (ab282302) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)
  • sELISA

Lab

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)

Interpolated concentrations of native Polymeric immunoglobulin receptor in human stomach tissue extract based on a 500 µg/mL extract load. The concentrations of Polymeric immunoglobulin receptor were measured in duplicate and interpolated from the Polymeric immunoglobulin receptor standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Polymeric immunoglobulin receptor concentration was determined to be 11.18 ng/mL in stomach tissue extract.

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)
  • sELISA

Lab

Sandwich ELISA - Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (AB325725)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 40.88 pg/mL

Range

109.74 - 80000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (ab325725) is a rapid single-wash sandwich 55-min ELISA to measure Human Polymeric immunoglobulin receptor (PIGR) in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Tissue Extract with a sensitivity of 40.88 pg/mL.

How the assay works

Human Polymeric immunoglobulin receptor (PIGR) ELISA Kit, Chemiluminescent (ab325725) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Polymeric immunoglobulin receptor (PIGR) (ab325725) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PIGR antibody [EPR23314-46] - BSA and Aside free (Capture) ab281273
- Anti-PIGR antibody [EPR23314-243] - BSA and Aside free (Detector) ab281123

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5" } ]
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Recovery

[ { "sample": "Serum", "range": "82 - 87 %", "average": "= 85" }, { "sample": "Citrate plasma", "range": "81 - 98 %", "average": "= 90" }, { "sample": "EDTA Plasma", "range": "80 - 87 %", "average": "= 83" }, { "sample": "Heparin Plasma", "range": "81 - 92 %", "average": "= 97" }, { "sample": "Cell culture media", "range": "85 - 89 %", "average": "= 87" }, { "sample": "Tissue Extracts", "range": "108 - 117 %", "average": "= 112" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Polymeric immunoglobulin receptor. Mediates selective transcytosis of polymeric IgA and IgM across mucosal epithelial cells. Binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process, a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.. Secretory component. Through its N-linked glycans ensures anchoring of secretory IgA (sIgA) molecules to mucus lining the epithelial surface to neutralize extracellular pathogens (PubMed : 12150896). On its own (free form) may act as a non-specific microbial scavenger to prevent pathogen interaction with epithelial cells (PubMed : 16543244).
See full target information PIGR
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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