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AB283990

Human PPM1F/POPX2 ELISA Kit

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Human PPM1F/POPX2 ELISA Kit is a Sandwich ELISA for the measurement of Human PPM1F/POPX2 in Human in Biofluids samples.

View Alternative Names

KIAA0015, POPX2, PPM1F, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, Partner of PIX 2, Protein fem-2 homolog, CaM-kinase phosphatase, CaMKPase, hFem-2

1 Images
Sandwich ELISA - Human PPM1F/POPX2 ELISA Kit (AB283990)
  • sELISA

Supplier Data

Sandwich ELISA - Human PPM1F/POPX2 ELISA Kit (AB283990)

Example of Human PPM1F/POPX2 standard curve.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Qualitative

Sensitivity

= 0.45 ng/mL

Range

0.781 - 200 ng/mL

Assay time

5h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human PPM1F/POPX2 ELISA Kit (ab283990) is designed for detection of PPM1F/POPX2 in human plasma and serum samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures human PPM1F in approximately 5 hours. A polyclonal antibody specific for human PPM1F has been pre-coated onto a 96-well microplate with removable strips. PPM1F in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human PPM1F, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PPM1F also known as POPX2 is a protein phosphatase that belongs to the PP2C family. It functions as a serine/threonine phosphatase and has a molecular mass of approximately 48 kDa. PPM1F is expressed across various tissues including the brain muscle and liver. It plays a role in dephosphorylating proteins involved in numerous cellular processes. By regulating phosphorylation status PPM1F impacts cellular activities such as cell cycle progress and apoptotic pathways.
Biological function summary

This protein phosphatase is instrumental in modulating signaling events related to cytoskeletal dynamics and stress responses. PPM1F does not operate in isolation but rather interacts with other proteins to form complexes that fine-tune its activity and stability. For instance PPM1F associates with components of the actin cytoskeleton and stress-related kinases influencing cellular movement and survival responses. Its ability to deactivate key kinases highlights its biological importance in maintaining cellular function.

Pathways

Recent research places PPM1F in mechanistic contexts related to the MAPK signaling and apoptosis pathways. It interacts closely with proteins like MAPKs and CAMKII to mediate responses to external stimuli influencing cellular proliferation and stress outcomes. The modulation of these pathways by PPM1F highlights its role in facilitating the balance between cell survival and apoptotic signaling. This phosphatase therefore has a significant regulatory function in cellular signaling networks.

Alterations in PPM1F function have correlations with cancer and neurodegenerative diseases. In cancer PPM1F's dephosphorylation roles impact pathways relevant to cell proliferation and apoptosis contributing to tumor progression. Abnormal regulation of PPM1F has also been observed in certain neurodegenerative disorders where its interactions with proteins such as MAPKs affect neuronal survival and distress responses. The link between PPM1F and these conditions highlights its potential as a therapeutic target in disease management.
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Product protocols

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Target data

Dephosphorylates and concomitantly deactivates CaM-kinase II activated upon autophosphorylation, and CaM-kinases IV and I activated upon phosphorylation by CaM-kinase kinase. Promotes apoptosis.
See full target information PPM1F
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websiteProtocolBooklet
en
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Product promise

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