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AB284018

Human PR3 Autoantibody ELISA Kit

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Human PR3 Autoantibody ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human PR3 Autoantibody in Human in Biofluids samples.

View Alternative Names

MBN, PRTN3, Myeloblastin, AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, P29, Wegener autoantigen, PR-3, PR3, NP-4

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Sandwich ELISA - Human PR3 Autoantibody ELISA Kit (AB284018)
  • sELISA

Supplier Data

Sandwich ELISA - Human PR3 Autoantibody ELISA Kit (AB284018)

Example of Human PR3 Autoantibody standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.44 AU/mL

Range

1.875 - 30 AU/mL

Assay time

4h

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

The Human Proteinase 3 Autoantibody ELISA (ab284018) Kit is designed for the quantitative determination of autoimmune response (IgG) to a target antigen (PR3). The kit detects autoantibodies in plasma and serum samples.

This assay employs a quantitative sandwich enzyme immunoassay technique that measures autoantibodies (anti-PR3 IgG) in approximately 4 hours. A PR3 antigen has been pre-coated onto a 96-well microplate with removable strips. An autoantibody specific for PR3 in standards and samples is sandwiched by the immobilized antigen and a biotinylated polyclonal antibody specific for human IgG, which is recognized by a streptavidin-peroxidase (SP) conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.6" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 10, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.4" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab284018-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"10X Diluent M Concentrate", "number":"AB284018-CMP01", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB284018-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB284018-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB284018-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Autoimmune Biotinylated Human IgG Antibody", "number":"AB284018-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human PR3 Autoantibody Microplate", "number":"AB284018-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"PR3 Autoantibody Standard", "number":"AB284018-CMP07", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB284018-CMP10", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB284018-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.
Biological function summary

Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.

Pathways

Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.

PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.
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Product protocols

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Target data

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed : 2033050, PubMed : 28240246, PubMed : 3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed : 23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed : 22266279).
See full target information PRTN3
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