JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB226902

Human PR3 ELISA Kit

Be the first to review this product! Submit a review

|

(1 Publication)

Human PR3 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PR3 with a sensitivity of 150 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MBN, PRTN3, Myeloblastin, AGP7, C-ANCA antigen, Leukocyte proteinase 3, Neutrophil proteinase 4, P29, Wegener autoantigen, PR-3, PR3, NP-4

4 Images
Sandwich ELISA - Human PR3 ELISA Kit (AB226902)
  • sELISA

Supplier Data

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)

Example of Human PR3 standard curve in Sample Diluent NS

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)
  • sELISA

Supplier Data

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)

Representative image of Human PR3 ELISA Kit (ab226902)

Components shown from left to right :

- 10X Wash Buffer PT (ab206977)

- 10X Human PR3 ELISA Kit Capture Antibody

- 10X Human PR3 Detector Antibody

- 10X Human PR3 ELISA Kit Lyophilised Purified Protein (2 vials)

- Human PR3 ELISA Kit 50X Cell Extraction Enhancer Solution (ab193971)

- Sample diluent NS

- TMB solution

- Stop Solution

- 5X Cell Extraction Buffer PTR (ab193970)

- Antibody Diluent 4BI

SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.

Plate Seals - shown underneath bottles / vials.

Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.

Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)
  • sELISA

Supplier Data

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)

Interpolated concentrations of native PR3 in Human cell extracts and cell culture supernatant samples.

The concentrations of PR3 were measured in duplicates, interpolated from the PR3 standard curves and corrected for sample dilution. Undiluted samples are as follows : THP-1 cell extract (100 g/ml); HL-60 (50 g/ml); untreated THP-1 cell supernatant (100 %) and untreated Human PBMC supernatant (50%). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PR3 concentration was determined to be 24.62 ng/mL in THP-1 cell extract, 72.65 ng/mL in HL-60 cell extract and 6.16 ng/mL in THP-1 supernatant and 146.21 ng/ml in PBMC supernatant.

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)
  • sELISA

Supplier Data

Sandwich ELISA - Human PR3 ELISA Kit (AB226902)

Example of Human PR3 standard curve in Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 150 pg/mL

Range

1.2 - 150 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human PR3 ELISA Kit (ab226902) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PR3 protein in cell culture extracts and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PR3 with 150 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11" }, { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "90 - 117 %", "average": "= 101" }, { "sample": "Cell culture extracts", "range": "87 - 93 %", "average": "= 89" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab226902-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB226902-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB226902-CMP12", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB226902-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB226902-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB226902-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB226902-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PR3 Capture Antibody", "number":"AB226902-CMP02", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB226902-CMP10", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PR3 Lyophilized Recombinant Protein", "number":"AB226902-CMP04", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB226902-CMP09", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PR3 Detector Antibody", "number":"AB226902-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB226902-CMP05", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Proteinase 3 (PR3) also known as PRTN3 is a serine protease with a mass of approximately 29 kDa. It belongs to the human neutrophil elastase (HNE) family and is mainly expressed in neutrophils. Within these immune cells PR3 localizes to azurophilic granules where it participates in early immune responses. The proteolytic activity of PR3 involves cleaving peptide bonds in target proteins contributing to the processing and degradation of various proteins important for immune function.
Biological function summary

Proteinase 3 serves multiple roles including modulating inflammation and immune responses. PR3 functions as part of a complex with azurophilic granules aiding in microbial defense mechanisms. In addition the protease regulates cytokine activation and deactivation which in turn influences cell signaling related to inflammation. Its enzymatic action ensures that PR3 can manage inflammatory processes by breaking down extracellular matrix components thereby allowing white blood cells to access infection sites.

Pathways

Proteinase 3 plays an important role within the serine protease and inflammatory pathways. It interacts with other proteins such as neutrophil elastase with which it shares a pathway to modulate immune responses. PR3 also partners with cathepsin G contributing to processes such as pathogen clearance and tissue remodeling providing a coordinated response during infection and injury. These interactions show PR3's involvement in regulating the balance between inflammation and tissue repair.

PR3 is notably associated with Wegener's granulomatosis also known as granulomatosis with polyangiitis. This autoimmune condition results in systemic vasculitis where anti-proteinase 3 antibodies notably characterize the disease. Additionally PR3 plays a part in chronic obstructive pulmonary disease (COPD) where its proteolytic activity contributes to tissue damage in the lungs. In both conditions PR3 interplays with related proteins like myeloperoxidase further demonstrating its importance in processes leading to these disorders.
style
left-column

Product protocols

style
left-column

Target data

Serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) (PubMed : 2033050, PubMed : 28240246, PubMed : 3198760). By cleaving and activating receptor F2RL1/PAR-2, enhances endothelial cell barrier function and thus vascular integrity during neutrophil transendothelial migration (PubMed : 23202369). May play a role in neutrophil transendothelial migration, probably when associated with CD177 (PubMed : 22266279).
See full target information PRTN3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 12:686462 PubMed34276672

2021

Enhanced Expression of Autoantigens During SARS-CoV-2 Viral Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Narjes Saheb Sharif-Askari,Fatemeh Saheb Sharif-Askari,Samrein B M Ahmed,Suad Hannawi,Rifat Hamoudi,Qutayba Hamid,Rabih Halwani
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB272101

Human Myeloperoxidase ELISA Kit

Recombinant|SimpleStep

ELISA - Human Myeloperoxidase ELISA Kit (AB272101)

  • 0
  • 0
Assay Kits

AB270204

Human Neutrophil Elastase ELISA Kit

Recombinant|SimpleStep

ELISA - Human Neutrophil Elastase ELISA Kit (AB270204)

  • 0
  • 0
Assay Kits

AB260058

Human CRP ELISA Kit (C-Reactive protein)

Recombinant|SimpleStep

ELISA - Human CRP ELISA Kit (C-Reactive protein) (AB260058)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com