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AB234561

Human PRAME ELISA Kit

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(1 Publication)

Human PRAME ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PRAME with a sensitivity of 83 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

MAPE, OIP4, PRAME, Melanoma antigen preferentially expressed in tumors, Opa-interacting protein 4, Preferentially expressed antigen of melanoma, OIP-4

3 Images
ELISA - Human PRAME ELISA Kit (AB234561)
  • ELISA

Supplier Data

ELISA - Human PRAME ELISA Kit (AB234561)
Sandwich ELISA - Human PRAME ELISA Kit (AB234561)
  • sELISA

Supplier Data

Sandwich ELISA - Human PRAME ELISA Kit (AB234561)

Interpolated concentrations of native PRAME in human K562 cell extract based on a 100 µg/mL extract load and testis tissue extract based on a 180 μg/mL extract load.

The concentrations of PRAME were measured in duplicate and interpolated from the PRAME standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PRAME concentration was determined to be 14.70 ng/mL in K562 cell extract and 2.359 ng/mL in testis tissue extract.

Sandwich ELISA - Human PRAME ELISA Kit (AB234561)
  • sELISA

Supplier Data

Sandwich ELISA - Human PRAME ELISA Kit (AB234561)

Example of human PRAME standard curve in 1X Cell Extraction Buffer + Enhancer.

The PRAME standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell culture extracts, Tissue Extracts

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 83 pg/mL

Range

0.313 - 20 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PRAME ELISA Kit ab234561 is a rapid single-wash 90-min Sandwich ELISA to measure Human PRAME in cell culture extracts, tissue extracts. This SimpleStep sensitivity is 83 pg/mL.

How the assay works

Human PRAME SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human PRAME ELISA Kit ab234561 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PRAME antibody [EPR20366-113] - BSA and Azide free (Capture) ab245019
- Anti-PRAME antibody [EPR20366-80] - BSA and Azide free (Detector) ab245019

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Cell Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Cell Extract", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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Recovery

[ { "sample": "Cell culture extracts", "range": "104 - 106 %", "average": "= 105" }, { "sample": "Tissue Extracts", "range": "94 - 96 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab234561-1x96Tests", "assetComponentDetails": [ { "size":"1 x 500 µL", "name":"Denaturant", "number":"AB234561-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB234561-CMP07", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB234561-CMP15", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB234561-CMP14", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PRAME Capture Antibody", "number":"AB234561-CMP03", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB234561-CMP13", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB234561-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB234561-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PRAME Detector Antibody", "number":"AB234561-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PRAME Lyophilized Recombinant Protein", "number":"AB234561-CMP05", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB234561-CMP11", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB234561-CMP10", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB234561-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PRAME also known as Preferentially Expressed Antigen in Melanoma is a protein typically weighing around 50 kDa. It is found mostly in testis tissue but also appears in several types of tumors including melanoma and some leukemias. PRAME does not show significant expression in most normal tissues making it a potential target for cancer therapies. Immunohistochemistry (IHC) techniques such as PRAME IHC and PRAME staining often use monoclonal antibodies like mAb EPR20330 to detect PRAME protein presence in various samples.
Biological function summary

PRAME acts as a repressor of retinoic acid signaling a process important for cell differentiation and growth. It does not directly form part of a complex but interacts with components involved in retinoic acid pathways. By binding to retinoic acid receptor complexes PRAME prevents activation of genes involved in cell cycle arrest and apoptosis contributing to unchecked cellular proliferation seen in certain cancers.

Pathways

PRAME participates in the retinoic acid and various oncogenic signaling processes. It influences the retinoic acid pathway by interfering with the retinoic acid receptor (RAR) signaling. This interference with key proteins like RAR disrupts the normal regulatory processes that typically inhibit cancer progression. PRAME's modulation of these pathways highlights its role in promoting tumor growth and survival.

PRAME has significant relevance to cancer particularly melanoma. It is often used as a biomarker for melanoma identification due to its overexpression in such tumors. Additionally PRAME's connection to acute myeloid leukemia (AML) emerges from its ability to act similarly in diverse malignant cells. In these diseases the overexpression of PRAME interferes with normal differentiation processes and allows for sustained proliferation of malignant cells suggesting its potential role as a therapeutic target or diagnostic marker in oncology.
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Product protocols

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Target data

The protein expressed by gene PRAME functions as a transcriptional repressor, inhibiting retinoic acid signaling through retinoic acid receptors RARA, RARB, and RARG. It prevents retinoic acid-induced cell proliferation arrest, differentiation, and apoptosis. This supplementary information is collated from multiple sources and compiled automatically.
See full target information PRAME
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials science 13:2908-2924 PubMed40338561

2025

Development of a nano-vaccine for high-grade serous ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chayanika Saha,Ahmed Elkashif,Elaine J Gilmore,Binyumeng Jiang,Ying Sun,Raj Kumar Duary,Niamh Buckley,Nicholas J Dunne,Helen O McCarthy
View all publications
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websiteProtocolBooklet
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