JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB231920

Human Prealbumin ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Prealbumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prealbumin with a sensitivity of 6.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA

7 Images
Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

Example of human Prealbumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

Interpolated concentration of native Prealbumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.05% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

Example of human Prealbumin standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

Interpolated concentrations of native Prealbumin in human serum and plasma samples.

The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400,000, plasma (citrate) 1 : 400,000, plasma (heparin) 1 : 400,000, and plasma (EDTA) 1 : 400,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 273 μg/mL in serum, 220 μg/mL in plasma (citrate), 216 μg/ml in plasma (heparin), and 210 μg/ml in plasma (EDTA).

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

Interpolated concentrations of native Prealbumin in human cell culture supernatant, milk, and CSF samples.

The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 supernatant 1 : 500, milk 1 : 5,000, and CSF 1 : 500,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 0.3 μg/mL in HepG2 supernatant, 2.7 μg/ml in milk, and 20 μg/ml in cerebrospinal fluid.

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 594 μg/mL with a range of 303 – 1217 μg/mL.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 6.3 pg/mL

Range

12.5 - 800 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

You may be interested in:

AB316136

Human DAP12 (pY91) + DAP12 Total ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

Human Prealbumin ELISA Kit ab231920 is a rapid single-wash 90-min sandwich ELISA kit to measure Human Prealbumin in Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant, and Milk. This Simplestep sensitivity is 6.3 pg/mL.

How the assay works

Human Prealbumin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Human Prealbumin ELISA Kit ab231920 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Prealbumin antibody [EPR20073-79] - BSA and Azide free (Capture) ab244716
- Anti-Prealbumin antibody [EPR20073-82] - BSA and Azide free (Detector) ab244983

Related and Recommended products

Explore our extensive Alzheimer's research solutions here: https://www.abcam.com/en-us/products/product-recommendations/alzheimers-disease-tools
style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.6" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "101 - 119 %", "average": "= 107" }, { "sample": "Milk", "range": "98 - 108 %", "average": "= 103" }, { "sample": "Serum", "range": "92 - 99 %", "average": "= 95" }, { "sample": "EDTA Plasma", "range": "80 - 99 %", "average": "= 89" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 107 %", "average": "= 100" }, { "sample": "Heparin Plasma", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Citrate plasma", "range": "99 - 100 %", "average": "= 100" } ]
style
left-column

What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab231920-1x384Tests", "assetComponentDetails": [ { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB231920-CMP07" }, { "size":"1 x 600 µL", "name":"10X Human Prealbumin Capture Antibody", "number":"AB231920-CMP02" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231920-CMP05" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231920-CMP10" }, { "size":"2 x 1 Vial", "name":"Human Prealbumin Lyophilized Recombinant Protein", "number":"AB231920-CMP04" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB231920-CMP01" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB231920-CMP11" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231920-CMP09" }, { "size":"1 x 600 µL", "name":"10X Human Prealbumin Detector Antibody", "number":"AB231920-CMP03" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB231920-CMP08" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab231920-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB231920-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Prealbumin Capture Antibody", "number":"AB231920-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Prealbumin Lyophilized Recombinant Protein", "number":"AB231920-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB231920-CMP10", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB231920-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB231920-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Prealbumin Detector Antibody", "number":"AB231920-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB231920-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB231920-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB231920-CMP08", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.
style
left-column

Product protocols

style
left-column

Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
See full target information TTR
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
websiteProtocolBooklet
en
style
right-column

Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB108895

Human PreAlbumin ELISA Kit (Transthyretin)

elisa-kits

human-prealbumin-elisa-kit-transthyretin-ab108895

0

(0 reviews)

Alternative Version
Assay Kits

AB244071

Human Prealbumin Antibody Pair - BSA and Azide free

matched-antibody-pair-kits

human-prealbumin-antibody-pair-bsa-and-azide-free-ab244071

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com