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Human Prealbumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prealbumin with a sensitivity of 6.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

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Images

Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920), expandable thumbnail
  • Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920), expandable thumbnail
  • Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920), expandable thumbnail
  • Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920), expandable thumbnail
  • Sandwich ELISA - Human Prealbumin ELISA Kit (AB231920), expandable thumbnail

Key facts

Detection method
Colorimetric
Sample types
Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
12.5 - 800 pg/mL
Assay time
1h 30m
Sensitivity
= 6.3 pg/mL

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Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.

Alternative names

What's included?

1 x 384 Tests
Components
10X Human Prealbumin Capture Antibody
1 x 600 µL
10X Human Prealbumin Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
384 well CaptSure™ microplates
1 x 1 Unit
Antibody Diluent 5BI
1 x 6 mL
Human Prealbumin Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
2 x 250 mL
Stop Solution
2 x 12 mL
TMB Development Solution
2 x 12 mL

Recommended products

Human Prealbumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prealbumin with a sensitivity of 6.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

Key facts

Detection method
Colorimetric
Sample types
Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
Assay type
Sandwich (quantitative)
Reactive species
Human
Range
12.5 - 800 pg/mL
Assay time
1h 30m
Assay Platform
Pre-coated microplate (12 x 8 well strips)
Sensitivity
= 6.3 pg/mL

Precision

Intra assay

Sample
Overall
n
5
C.V.
4.6

Inter assay

Sample
Overall
n
4
C.V.
8.4

Recovery

Sample specific recovery

Sample type
Cell culture supernatant
Average %
= 107
Range
101 - 119 %
Sample type
Milk
Average %
= 103
Range
98 - 108 %
Sample type
Serum
Average %
= 95
Range
92 - 99 %
Sample type
EDTA Plasma
Average %
= 89
Range
80 - 99 %
Sample type
Cerebral Spinal Fluid
Average %
= 100
Range
95 - 107 %
Sample type
Heparin Plasma
Average %
= 101
Range
98 - 104 %
Sample type
Citrate plasma
Average %
= 100
Range
99 - 100 %

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Human Prealbumin ELISA Kit ab231920 is a rapid single-wash 90-min sandwich ELISA kit to measure Human Prealbumin in Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant, and Milk. This Simplestep sensitivity is 6.3 pg/mL.

How the assay works

Human Prealbumin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Human Prealbumin ELISA Kit ab231920 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Prealbumin antibody [EPR20073-79] - BSA and Azide free (Capture) Anti-Prealbumin antibody [EPR20073-79] - BSA and Azide free (Capture) ab244716
- Anti-Prealbumin antibody [EPR20073-82] - BSA and Azide free (Detector) Anti-Prealbumin antibody [EPR20073-82] - BSA and Azide free (Detector) ab244983

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.

Biological function summary

Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.

Pathways

Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.

Associated diseases and disorders

Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.

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7 product images

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    Example of human Prealbumin standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    Interpolated concentrations of native Prealbumin in human serum and plasma samples.

    The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 400,000, plasma (citrate) 1: 400,000, plasma (heparin) 1: 400,000, and plasma (EDTA) 1: 400,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 273 μg/mL in serum, 220 μg/mL in plasma (citrate), 216 μg/ml in plasma (heparin), and 210 μg/ml in plasma (EDTA).

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    Interpolated concentrations of native Prealbumin in human cell culture supernatant, milk, and CSF samples.

    The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 supernatant 1: 500, milk 1: 5,000, and CSF 1: 500,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 0.3 μg/mL in HepG2 supernatant, 2.7 μg/ml in milk, and 20 μg/ml in cerebrospinal fluid.

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 594 μg/mL with a range of 303 – 1217 μg/mL.

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    Interpolated concentration of native Prealbumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.05% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920), expandable thumbnail

    Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)

    Example of human Prealbumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

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