Human Prealbumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prealbumin with a sensitivity of 6.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 6.3 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Alternative names
PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA
What's included?
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Human Prealbumin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prealbumin with a sensitivity of 6.3 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 384-well plate for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 12.5 - 800 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 6.3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 5 | C.V. 4.6 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Overall | n 4 | C.V. 8.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 107 | Range 101 - 119 % |
Sample type Milk | Average % = 103 | Range 98 - 108 % |
Sample type Serum | Average % = 95 | Range 92 - 99 % |
Sample type EDTA Plasma | Average % = 89 | Range 80 - 99 % |
Sample type Cerebral Spinal Fluid | Average % = 100 | Range 95 - 107 % |
Sample type Heparin Plasma | Average % = 101 | Range 98 - 104 % |
Sample type Citrate plasma | Average % = 100 | Range 99 - 100 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Prealbumin ELISA Kit ab231920 is a rapid single-wash 90-min sandwich ELISA kit to measure Human Prealbumin in Serum, Heparin Plasma, Citrate plasma, EDTA Plasma, Cerebral Spinal Fluid, Cell culture supernatant, and Milk. This Simplestep sensitivity is 6.3 pg/mL.
How the assay works
Human Prealbumin SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Human Prealbumin ELISA Kit ab231920 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Prealbumin antibody [EPR20073-79] - BSA and Azide free (Capture) Anti-Prealbumin antibody [EPR20073-79] - BSA and Azide free (Capture) ab244716
- Anti-Prealbumin antibody [EPR20073-82] - BSA and Azide free (Detector) Anti-Prealbumin antibody [EPR20073-82] - BSA and Azide free (Detector) ab244983
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prealbumin also known as transthyretin is a protein with a molecular mass of approximately 55 kDa. The liver primarily synthesizes prealbumin and it is present in the bloodstream. Functionally prealbumin transports thyroxine and retinol-binding protein-retinol complex facilitating the distribution of these molecules to target tissues. As such it plays an integral role in maintaining retinol and thyroid hormone homeostasis.
- Biological function summary
Prealbumin interacts with retinol-binding protein to form a complex essential for vitamin A transportation. This complex prevents renal filtration of retinol-binding protein thereby conserving vitamin A in circulation. The protein's role extends beyond transport as it influences neurophysiological processes due to its ability to bind thyroid hormones and affect gene expression related to metabolism. Prealbumin is primarily a part of the thyroxine-transferring complex reinforcing its critical position in hormonal and vitamin dynamics.
- Pathways
Prealbumin's role in the thyroxine-binding globulin pathway highlights its importance in the endocrine system. Another pathway where prealbumin contributes significantly is the vitamin A metabolic pathway. In these pathways prealbumin maintains functional relationships with proteins like retinol-binding protein and thyroxine-binding globulin which jointly modulate thyroid hormone transport and vitamin A dynamics across cellular membranes.
- Associated diseases and disorders
Prealbumin directly associates with familial amyloid polyneuropathy and malnutrition. Familial amyloid polyneuropathy involves the deposition of prealbumin-derived amyloid fibrils in peripheral nerves impacting neuron function. In malnutrition decreased prealbumin levels serve as a biomarker reflecting poor nutritional status. Clinically prealbumin's relation to retinol-binding protein and thyroid hormone levels facilitates its use in diagnosing and monitoring these conditions.
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7 product images
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
Example of human Prealbumin standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
Interpolated concentrations of native Prealbumin in human serum and plasma samples.
The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1: 400,000, plasma (citrate) 1: 400,000, plasma (heparin) 1: 400,000, and plasma (EDTA) 1: 400,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 273 μg/mL in serum, 220 μg/mL in plasma (citrate), 216 μg/ml in plasma (heparin), and 210 μg/ml in plasma (EDTA).
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
Interpolated concentrations of native Prealbumin in human cell culture supernatant, milk, and CSF samples.
The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows: HepG2 supernatant 1: 500, milk 1: 5,000, and CSF 1: 500,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 0.3 μg/mL in HepG2 supernatant, 2.7 μg/ml in milk, and 20 μg/ml in cerebrospinal fluid.
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 594 μg/mL with a range of 303 – 1217 μg/mL.
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
Interpolated concentration of native Prealbumin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 0.05% HepG2 supernatant. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Sandwich ELISA - Human Prealbumin ELISA Kit (ab231920)
Example of human Prealbumin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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