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AB325455

Human Prealbumin ELISA Kit, Chemiluminescent

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Human Prealbumin ELISA Kit, Chemiluminescent is a 55-min single-wash Simplestep IgniteTM ELISA for the rapid quantification of Human Prealbumin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single -wash step for results in less than an hour
- Chemiluminescent detection allows a broad dynamic range :4.12 - 3000 pg/mL with a sensitivity of 1.8 pg/mL
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PALB, TTR, Transthyretin, ATTR, Prealbumin, TBPA

6 Images
Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)

Serum from ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 594 μg/mL with a range of 303 – 1,217 μg/mL.

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)

Interpolated concentrations of native Prealbumin in human serum and plasma samples. The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1 : 400,000, plasma (citrate) 1 : 400,000, plasma (heparin) 1 : 400,000, and plasma (EDTA) 1 : 400,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 273 μg/mL in serum, 220 μg/mL in plasma (citrate), 216 μg/ml in plasma (heparin), and 210 μg/ml in plasma (EDTA).

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)

Interpolated concentrations of native Prealbumin in human cell culture supernatant, milk, and CSF samples. The concentrations of Prealbumin were measured in duplicates, interpolated from the Prealbumin standard curves and corrected for sample dilution. Undiluted samples are as follows : HepG2 supernatant 1 : 500, milk 1 : 5,000, and CSF 1 : 50,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prealbumin concentration was determined to be 0.3 μg/mL in HepG2 supernatant, 2.7 μg/ml in milk, and 20 μg/ml in cerebrospinal fluid.

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)

Dynamic range comparison between the Chemiluminescent Human Prealbumin SimpleStep ELISA, colorimetric Human Prealbumin SimpleStep ELISA (ab231920), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)
  • sELISA

Lab

Sandwich ELISA - Human Prealbumin ELISA Kit, Chemiluminescent (AB325455)

Standard curve comparison between the Chemiluminescent Human Prealbumin SimpleStep ELISA and colorimetric Human Prealbumin SimpleStep ELISA (ab231920) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Luminescent

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Milk, Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 1.8 pg/mL

Range

4.12 - 3000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Prealbumin ELISA Kit, Chemiluminescent (ab325455) is a rapid single-wash sandwich 55-min ELISA to measure Human Prealbumin in Serum, Citrate Plasma, EDTA Plasma, Heparin Plasma, Cell Culture Supernatant, Milk, Cerebrospinal Fluid with a sensitivity of 1.8 pg/ml.

How the assay works

Human Prealbumin ELISA Kit, Chemiluminescent (ab325455) SimpleStep ELISA®  employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time.  By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colormetric ELISAs.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

Please refer to our protocol booklet for more details.

Human Prealbumin (ab325455) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature

2. Wash

3. Add prepared ChemiHRP development solution - incubate for 2 min

4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-TTR antibody [EPR20073-79] - BSA and Azide free (Capture) ab244716

- Anti-TTR antibody [EPR20073-155] - BSA and Azide free (Detector) ab244856

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.6" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 4, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.4" } ]
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Recovery

[ { "sample": "Serum", "range": "92 - 99 %", "average": "= 95" }, { "sample": "Citrate plasma", "range": "99 - 100 %", "average": "= 100" }, { "sample": "EDTA Plasma", "range": "98 - 104 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "80 - 99 %", "average": "= 89" }, { "sample": "Cell culture supernatant", "range": "101 - 119 %", "average": "= 107" }, { "sample": "Milk", "range": "98 - 108 %", "average": "= 103" }, { "sample": "Cerebral Spinal Fluid", "range": "95 - 107 %", "average": "= 100" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
See full target information TTR
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com