JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB202405

Human Prekallikrein 1B ELISA Kit

Be the first to review this product! Submit a review

|

(5 Publications)

Human Prekallikrein 1B ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Prekallikrein 1B with a sensitivity of 154 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK

6 Images
ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
  • ELISA

Supplier Data

ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)

Titration of human serum and plasma within the working range of the assay.

Background-subtracted data values (mean +/- SD, n = 2) are graphed.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)

Example of Prekallikrein 1B standard curve.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)

Interpolated concentrations of Prekallikrein 1B levels in individual healthy donors.

Serum from 10 healthy male donors was measured in duplicate. The mean Plasma Kallikrein 1B concentration was determined to be 112.6 μg/mL with a range of 80.8 – 206.4 μg/mL in male donors.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)

Linearity of dilution is determined based on interpolated values from the standard curve. Linearity of dilution defines a sample concentration interval in which interpolated target concentrations are directly proportional to sample dilution.

Native PreKallikrein 1B was measured in the following biological samples in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit (AB202405)

Example of Prekallikrein 1B standard curve. The Prekallikrein 1B standard curve was prepared as described. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 154 pg/mL

Range

0.47 - 30 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Human Prekallikrein 1B ELISA Kit ab202405 is a rapid single-wash 90-min Sandwich ELISA to measure Human Prekallikrein 1B in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, milk, serum. This SimpleStep sensitivity is 154 pg/mL.

How the assay works

Human Prekallikrein 1B SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Prekallikrein 1B ELISA Kit ab202405 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Prekallikrein 1B (KLKB1) is a 88 kDa serine protease involved in blood coagulation, kinin generation, fibrinolysis and inflammation. Prekallikrein 1B is synthesized in the liver and circulates in the blood stream as a free zymogen or in a complex with high molecular weight kininogen. Prekallikrein 1B has a signal peptide (aa1-19) and a pro form (aa20-638). It is composed of a heavy chain and a light chain which are cleaved upon activation.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.3" } ]
style
left-column

Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 101 %", "average": "= 94" }, { "sample": "Milk", "range": "82 - 91 %", "average": "= 85" }, { "sample": "Serum", "range": "84 - 98 %", "average": "= 92" }, { "sample": "EDTA Plasma", "range": "88 - 107 %", "average": "= 101" }, { "sample": "Heparin Plasma", "range": "87 - 97 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "87 - 95 %", "average": "= 92" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab202405-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB202405-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB202405-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB202405-CMP02", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB202405-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB202405-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Prekallikrein 1B Lyophilized Recombinant Protein", "number":"AB202405-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB202405-CMP08", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Prekallikrein 1B Capture Antibody", "number":"AB202405-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Prekallikrein 1B Detector Antibody", "number":"AB202405-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB202405-CMP03", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Plasma Kallikrein 1B also known as KLKB1 functions mechanically as a serine protease involved in the kinin-kallikrein system. It is pivotal for initiating pathways relating to inflammation and blood pressure regulation. Plasma Kallikrein 1B is active in plasma with a molecular mass around 88 kDa. It is predominantly expressed in endothelial cells and liver hepatocytes where its precursor form is activated.
Biological function summary

Plasma Kallikrein 1B participates in the cleavage of high molecular weight kininogen (HMWK) to release bradykinin a potent vasodilator. This protein is not typically part of a stable macromolecular complex but interacts transiently with other proteins during its activation and function. Its action facilitates increased vascular permeability which plays a role in inflammatory and coagulation responses.

Pathways

Plasma Kallikrein 1B mainly operates within the kallikrein-kinin system and the intrinsic coagulation pathway. It activates Factor XII (Hageman factor) which serves as an upstream regulator in the coagulation cascade. Additionally it connects to other proteins like prekallikrein and high molecular weight kininogen in these pathways coordinating responses in inflammation and blood pressure control.

Plasma Kallikrein 1B associates significantly with hereditary angioedema and diabetic retinopathy. Elevated activity or dysregulation of this protein contributes to excessive inflammatory responses seen in these conditions. It also interacts with C1 inhibitor proteins which regulate its activity under normal physiological conditions. This relationship emphasizes its role in maintaining balance within the kinin-kallikrein and related pathways which when disrupted can lead to pathophysiological conditions.
style
left-column

Product protocols

style
left-column

Target data

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
See full target information KLKB1
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Lipids in health and disease 22:79 PubMed37353816

2023

Circulating prekallikrein levels are correlated with lipid levels in the chinese population: a cross-sectional study.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanlu Shu,Xiang Zhao,Changshun Yang,Yan Yan,Yao Zheng,Xijie Wang,Chengfeng Qiu

Translational research : the journal of laboratory and clinical medicine 259:35-45 PubMed37085047

2023

Diabetes mellitus and aortic stenosis head to head: toward personalized medicine in patients with both pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Corbacho-Alonso,Tamara Sastre-Oliva,Luis F López-Almodovar,Jorge Solis,Luis R Padial,Teresa Tejerina,Montserrat Carrascal,Laura Mourino-Alvarez,Maria G Barderas

Journal of thrombosis and haemostasis : JTH 20:48-57 PubMed34532976

2021

Kallikrein augments the anticoagulant function of the protein C system in thrombin generation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Wan,Nadira Vadaq,Joke Konings,Martin Jaeger,Vinod Kumar,Bas de Laat,Leo Joosten,Mihai G Netea,Andre J van der Ven,Philip G de Groot,Quirijn de Mast,Mark Roest

Scientific reports 10:3844 PubMed32123229

2020

Serum proteomics of active tuberculosis patients and contacts reveals unique processes activated during Mycobacterium tuberculosis infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jesús Mateos,Olivia Estévez,África González-Fernández,Luis Anibarro,Ángeles Pallarés,Rajko Reljic,Tufária Mussá,Cremildo Gomes-Maueia,Artur Nguilichane,José M Gallardo,Isabel Medina,Mónica Carrera

Transplantation 103:1821-1833 PubMed30964836

2019

Brain Death Enhances Activation of the Innate Immune System and Leads to Reduced Renal Metabolic Gene Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Laura J Zitur,Peter J Chlebeck,Scott K Odorico,Juan S Danobeitia,Tiffany J Zens,Cees Van Kooten,Michael Eerhart,Jose A Reyes,Megan L Springer,Jennifer M Coonen,Kevin G Brunner,Saverio V Capuano,Anthony M D'Alessandro,Luis A Fernandez
View all publications
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Complementary Products

Assay Kits

AB241383

Human Fibrinogen ELISA Kit - high sensitivity

Recombinant|SimpleStep

ELISA - Human Fibrinogen ELISA Kit - high sensitivity (AB241383)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Assay Kits

AB108823

Human Complement C3 ELISA Kit

Sandwich ELISA - Human Complement C3 ELISA Kit (AB108823)

  • 5
  • 2
Assay Kits

AB309180

Human Haptoglobin ELISA Kit

SimpleStep

ELISA - Human Haptoglobin ELISA Kit (AB309180)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com