AB325999

Human Prekallikrein 1B ELISA Kit, Chemiluminescent

SimpleStep Ignite™ ELISA
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Human Prekallikrein 1B ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Prekallikrein 1B with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 85.73 - 62500 pg/ml with a sensitivity of 22.12 pg/mL.
- Patent pending

View Alternative Names

KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK

5 Images

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

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Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Standard curve comparison between the Chemiluminescent Human Prekallikrein 1B SimpleStep ELISA and colorimetric Human Prekallikrein 1B SimpleStep ELISA (ab202405) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Prekallikrein 1B concentration was determined to be 112.6 µg/mL with a range of 80.8 – 206.4 µg/mL in male donors.

sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Titration of human serum and plasma within the working range of the assay. Background-subtracted data values (mean +/- SD, n = 2) are graphed.

sELISA

Lab

Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Chemiluminescent (AB325999)

Dynamic range comparison between the Chemiluminescent Human Prekallikrein 1B SimpleStep ELISA and colorimetric Human Prekallikrein 1B SimpleStep ELISA (ab202405). The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and dynamic range.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Milk, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 22.12 pg/mL

Range

85.73 - 62500 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Prekallikrein 1B ELISA Kit, Chemiluminescent (ab325999) is a rapid single-wash sandwich 55-min ELISA to measure Human Prekallikrein 1B in Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Milk, Cell culture supernatant with a sensitivity of 22.12 pg/mL.

How the assay works

Human Prekallikrein 1B ELISA Kit, Chemiluminescent (ab325999) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Prekallikrein 1B (ab325999) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

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Precision

[ { "reproducibilityType": "Intra", "sample": "Supernatant", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 6.3" }, { "reproducibilityType": "Inter", "sample": "Supernatant", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.8" } ]

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Recovery

[ { "sample": "Serum", "range": "87 - 101 %", "average": "= 94" }, { "sample": "EDTA Plasma", "range": "82 - 91 %", "average": "= 85" }, { "sample": "Heparin Plasma", "range": "94 - 98 %", "average": "= 92" }, { "sample": "Citrate plasma", "range": "88 - 107 %", "average": "= 101" }, { "sample": "Milk", "range": "87 - 97 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "87 - 95 %", "average": "= 92" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

See full target information KLKB1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com