Human Prekallikrein 1B ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Prekallikrein 1B with a sensitivity of 76 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
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Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.12 - 60 ng/mL
- Assay time
- 1h 30m
- Sensitivity
- = 76 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.
Alternative names
KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK
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Recommended products
Human Prekallikrein 1B ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Prekallikrein 1B with a sensitivity of 76 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.12 - 60 ng/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 76 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 5 | C.V. 6.3 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 5.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 94 | Range 87 - 101 % |
Sample type Milk | Average % = 85 | Range 82 - 91 % |
Sample type Serum | Average % = 92 | Range 84 - 98 % |
Sample type EDTA Plasma | Average % = 101 | Range 88 - 107 % |
Sample type Heparin Plasma | Average % = 91 | Range 87 - 97 % |
Sample type Citrate plasma | Average % = 92 | Range 87 - 95 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Prekallikrein 1B in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Plasma Kallikrein 1B protein in human serum, plasma, milk, and cell culture supernatant.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Prekallikrein 1B (KLKB1) is a 88 kDa serine protease involved in blood coagulation, kinin generation, fibrinolysis and inflammation. Mature KLKB1 primarily circulates as a complex with high-molecular weight kinnogen, where it releases kinins from kininogens during the inflammation and vasodilation response.
This kit detects both prekallikrein and plasma kallikrein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.
- Biological function summary
Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.
- Pathways
Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.
- Associated diseases and disorders
Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.
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3 product images
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (ab229411)
Example of human Prekallikrein 1B standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (ab229411)
Titration of human serum and plasma within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (ab229411)
Interpolated concentrations of Prekallikrein 1B levels in individual healthy donors.
Serum from 10 healthy male donors was measured in duplicate. The mean Plasma Kallikrein 1B concentration was determined to be 112.6 μg/mL with a range of 80.8 – 206.4 μg/mL in male donors.
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