Human Prekallikrein 1B ELISA Kit, Fluorescent
- CatchPoint SimpleStep ELISA
- What is this?
Be the first to review this product! Submit a review
|
(0 Publication)
Human Prekallikrein 1B ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Prekallikrein 1B with a sensitivity of 76 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
View Alternative Names
KLK3, KLKB1, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, PKK
- ELISA
Supplier Data
ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (AB229411)
- sELISA
Supplier Data
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (AB229411)
Example of human Prekallikrein 1B standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (AB229411)
Interpolated concentrations of Prekallikrein 1B levels in individual healthy donors.
Serum from 10 healthy male donors was measured in duplicate. The mean Plasma Kallikrein 1B concentration was determined to be 112.6 μg/mL with a range of 80.8 – 206.4 μg/mL in male donors.
- sELISA
Supplier Data
Sandwich ELISA - Human Prekallikrein 1B ELISA Kit, Fluorescent (AB229411)
Titration of human serum and plasma within the working range of the assay.
Background-subtracted data values (mean +/- SD, n = 2) are graphed.
Complementary Products
Reactivity data
Product details
Human Prekallikrein 1B ELISA Kit, Fluorescent ab229411 is a rapid single-wash 90-min sandwich ELISA to measure Human Prekallikrein 1B in serum, heparin plasma, citrate plasma, EDTA Plasma, cell culture supernatant, milk.. This CatchPoint SimpleStep sensitivity is 76 pg/mL.
How the assay works
Human Prekallikrein 1B CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission. This kit is optimised for Molecular Devices Readers: If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human Prekallikrein 1B ELISA Kit, Fluorescent ab229411 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a fluorescent plate reader at 530/570/590 nm Excitation/Cutoff/Emission.
Colorimetric version
This kit is also available with a colorimetric readout as Human Prekallikrein 1B ELISA Kit ab202405.
Both the fluroescent and colorimetric version share the same antibody pair and protein standard.
Prekallikrein 1B (KLKB1) is a 88 kDa serine protease involved in blood coagulation, kinin generation, fibrinolysis and inflammation. Mature KLKB1 primarily circulates as a complex with high-molecular weight kinnogen, where it releases kinins from kininogens during the inflammation and vasodilation response.
This kit detects both prekallikrein and plasma kallikrein.
Precision
Recovery
What's included?
Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.
Pathways
Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
- Download websiteProtocolBooklet|en
Target data
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com