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Human Prekallikrein and Kallikrein ELISA Kit ab171015 is used to quantify Human Prekallikrein and Kallikrein with a sensitivity of 19.64 pg/ml.

Sandwich ELISA Kit

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Sandwich ELISA - Human Prekallikrein and Kallikrein ELISA Kit (AB171015), expandable thumbnail

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Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Assay time

4h

Sensitivity

= 0.43 ng/mL

Reactivity data

Application

sELISA

Reactivity

Reacts

Dilution info

-

Notes

-

Target data

Function

Participates in the surface-dependent activation of blood coagulation. Activates, in a reciprocal reaction, coagulation factor XII/F12 after binding to negatively charged surfaces. Releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin.

Alternative names

What's included?

1 x 96 Tests
Components
100X Streptavidin-Peroxidase Conjugate
1 x 80 µL
10X Diluent N Concentrate
1 x 30 mL
20X Wash Buffer Concentrate
2 x 30 mL
50X Biotinylated Human Prekallikrein Antibody
1 x 120 µL
Chromogen Substrate
1 x 7 mL
Prekallikrein Microplate (12 x 8 well strips)
1 x 1 Unit
Prekallikrein Standard
1 x 1 Vial
Sealing Tapes
3 x 1 Unit
Stop Solution
1 x 11 mL

Recommended products

Human Prekallikrein and Kallikrein ELISA Kit ab171015 is used to quantify Human Prekallikrein and Kallikrein with a sensitivity of 19.64 pg/ml.

Sandwich ELISA Kit

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Milk, Plasma, Saliva, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Assay time

4h

Sensitivity

= 0.43 ng/mL

General recovery

101%

Precision

Intra assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

4.6

Inter assay

Sample

Overall

n

0

mean

-

SD

-

C.V.

9.8

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

Multi

Storage information

Please refer to protocols

Notes

Abcam's Prekallikrein and Kallikrein Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Prekallikrein and Kallikrein levels in plasma, serum, saliva, milk, and cerebrospinal fluid samples.

A Prekallikrein specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Prekallikrein specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Prekallikrein and Kallikrein captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Plasma Kallikrein 1B also known as PKK or KLKB1 is a serine protease enzyme with an approximate mass of 88 kDa. It is important for the blood coagulation process. This protein is mainly expressed in the liver and circulates in the blood as the inactive precursor prekallikrein. Through cleavage prekallikrein converts into its active form plasma kallikrein. Plasma kallikrein exhibits various proteolytic activities influencing multiple substrates involved in hemostatic and inflammatory responses.

Biological function summary

Plasma Kallikrein 1B plays significant roles in the kinin-kallikrein system. Within this system it cleaves high-molecular-weight kininogen to release bradykinin a potent vasodilator. This enzyme is not part of a larger protein complex but operates independently to modulate various physiological responses. Plasma kallikrein's activity influences processes like pain vasodilation and inflammation making it important in homeostasis and acute-phase responses.

Pathways

Plasma Kallikrein 1B participates actively in the kallikrein-kinin and the contact activation pathways. In the kallikrein-kinin system it works alongside high-molecular-weight kininogen and factor XII which plays an important role in the intrinsic pathway of coagulation. Plasma kallikrein's enzymatic activities lead to the production of bradykinin and other peptides impacting blood pressure and inflammatory processes.

Associated diseases and disorders

Plasma Kallikrein 1B associates strongly with hereditary angioedema and diabetic retinopathy. In hereditary angioedema its dysregulation leads to excessive bradykinin production causing episodes of swelling. In diabetic retinopathy elevated plasma kallikrein activity can exacerbate vascular complications. Plasma kallikrein's disorderly activities also connect it to other proteins like C1 inhibitor in these pathological conditions highlighting its importance in clinical studies.

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1 product image

  • Sandwich ELISA - Human Prekallikrein and Kallikrein ELISA Kit (ab171015), expandable thumbnail

    Sandwich ELISA - Human Prekallikrein and Kallikrein ELISA Kit (ab171015)

    Representative Standard Curve using ab171015

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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