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AB277476

Human Presenilin 2/AD5 ELISA Kit

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Human Presenilin 2/AD5 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Presenilin 2/AD5 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

AD4, PS2, PSNL2, STM2, PSEN2, Presenilin-2, PS-2, AD3LP, AD5, E5-1, STM-2

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ELISA - Human Presenilin 2/AD5 ELISA Kit (AB277476)
  • ELISA

Supplier Data

ELISA - Human Presenilin 2/AD5 ELISA Kit (AB277476)

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.2 ng/mL

Range

0.205 - 50 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Presenilin 2/AD5 ELISA Kit (ab277476) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Presenilin 2/AD5 in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human Presenilin 2/AD5 coated on a 96-well plate. Standards and samples are pipetted into the wells and Human Presenilin 2/AD5 present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti-Human Presenilin 2/AD5 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Human Presenilin 2/AD5 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "83 - 141 %", "average": "= 110.1" }, { "sample": "Plasma", "range": "70 - 127 %", "average": "= 99.89" }, { "sample": "Cell culture media", "range": "71 - 110 %", "average": "= 91" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab277476-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB277476-CMP01", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB277476-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Anti-Human Presenilin 2 AD5 coated Microplate", "number":"AB277476-CMP04", "productcode":"" }, { "size":"1 x 200 µL", "name":"800X HRP-Streptavidin Concentrate", "number":"AB277476-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Presenilin 2/AD5 standard protein (Lyophilized)", "number":"AB277476-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Anti-Human Presenilin 2/AD5 Antibody", "number":"AB277476-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB277476-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB277476-CMP08", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB277476-CMP05", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The presenilin 2 (PSEN2) protein also known as AD5 plays a role as a catalytic subunit in the gamma-secretase complex. This complex is involved in intramembrane proteolysis of several type-I transmembrane proteins. PSEN2 has a mass of about 52 kDa and is expressed mainly in the brain and several other tissues throughout the body. Its gene is located on chromosome 1 and has the alternative name 'EP-2614'. Presenilin 2 is closely associated with presenilin 1 (PSEN1) a related protein within the same family.
Biological function summary

Presenilin 2 functions in the regulation of proteolytic processing of the amyloid precursor protein (APP) within the gamma-secretase complex. This action produces amyloid beta peptides which are critical for normal brain function but may accumulate under pathological conditions. The protein is also involved in NOTCH receptor activation which plays a part in cell fate determination during development. The gamma-secretase complex includes other key proteins such as nicastrin anterior pharynx-defective 1 (APH-1) and presenilin enhancer 2 (PEN-2).

Pathways

Presenilin 2 is central to the amyloidogenic pathway through its role in APP processing. This pathway links to Alzheimer's disease where dysregulation of amyloid beta peptide production leads to plaque formation. Additionally presenilin 2 takes part in the NOTCH signaling pathway which influences neurogenesis and cellular differentiation. Presenilin 1 is a partner protein of presenilin 2 within these pathways sharing overlapping functions and regulation.

Presenilin 2's connection to Alzheimer's disease arises from its role in amyloid beta production. Mutations in the PSEN2 gene can lead to familial Alzheimer's disease where altered processing of APP results in neurotoxic peptide accumulation. Additionally presenilin 2 influences cardiovascular disease development through its involvement in cell signaling pathways affecting vascular biology. Presenilin 1 mutations also associate with similar disorders due to its analogous role and participation in these pathological processes.
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Product protocols

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Target data

Probable catalytic subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (amyloid-beta precursor protein). Requires the other members of the gamma-secretase complex to have a protease activity. May play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. May function in the cytoplasmic partitioning of proteins. The holoprotein functions as a calcium-leak channel that allows the passive movement of calcium from endoplasmic reticulum to cytosol and is involved in calcium homeostasis (PubMed : 16959576). Is a regulator of mitochondrion-endoplasmic reticulum membrane tethering and modulates calcium ions shuttling between ER and mitochondria (PubMed : 21285369).
See full target information PSEN2
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websiteProtocolBooklet
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