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AB210966

Human Pro-Collagen I alpha 1 ELISA Kit

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(1 Review)

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(53 Publications)

Human Pro-Collagen I alpha 1 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human pro-collagen I alpha 1 with a sensitivity of 5.3 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.

- Colorimetric sandwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, plasma, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 40 publications

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

14 Images
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Interpolated concentrations of native Pro-Collagen I alpha 1 in human IMR-90 extract based on a 2 μg/mL extract load.

The concentrations of Pro‑Collagen I alpha 1 were measured in duplicate and interpolated from the Pro‑Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1.62 ng/mL in IMR-90 extract.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Unknown

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Raw value data for example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.

Raw data values are shown in the table.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Serum from ten individual healthy human female donors was diluted 1 : 200 and measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 197.3 ng/mL with a range of 113.0 – 417 ng/mL.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Interpolated concentrations of native Pro-Collagen I alpha 1 in human serum and plasma samples.

The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 142.1 ng/mL in serum, 135.9 ng/mL in plasma (citrate), 112.1 ng/mL in plasma (EDTA) and 102.1 ng/mL in plasma (heparin).

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Unknown

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Raw value data for example of human Pro-Collagen I alpha 1 standard curve in 1X Cell Extraction Buffer PTR

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Unknown

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Linearity of dilution - native human Pro-Collagen I alpha 1 in human serum, plasma (citrate, EDTA, Heparin) and IMR-90 Extract.

Native human Pro-Collagen I alpha 1 was measured in human serum, plasma, and IMR-90 lysate in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for the IMR-90 lysate.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Unknown

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Linearity of dilution - recombinant human Pro-Collagen I alpha in cell culture media.

Recombinant human Pro-Collagen I alpha 1 was spiked into cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Lab

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2 µg/mL IMR-90 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Lab

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Example of human Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Lab

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Lab

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

Example of human Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate.

Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)
  • sELISA

Lab

Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (AB210966)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 5.3 pg/mL

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Pro-Collagen I alpha 1 ELISA Kit ab210966 is a rapid single-wash 90-min ELISA kit to measure Human Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 5.3 pg/mL.

How the assay works

Human Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

This kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.

Human Pro-Collagen I alpha 1 ELISA Kit ab210966 protocol summary:

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Pro-Collagen I alpha 1/ COL1A1 ELISA kit ab210966

Human Pro-Collagen I alpha 1/ COL1A1 ELISA kit ab210966 has been used to study cartilage regeneration and osteoarthritis treatment(1)and the effects of cigarette smoke and the sun on skin aging(2).

References:

(1)Kerong Dai et al. 2022, PMID: 36114225,
(2)Roxane Pouliot et al. 2023, PMID: 37863977

Save your precious samples

Our 384-well Human Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation

- Human Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free ab241825
- Anti-Procollagen Type 1 antibody [EPR19126-161] - BSA and Azide free (Capture) ab242540
- Anti-Procollagen Type 1 antibody [EPR19126-152] - BSA and Azide free (Detector) ab242788

Related and recommended products

Human Pro-Collagen I alpha 1/COL1A1 ELISA kit ab210966 is commonly used to study tissue structure and function. Human Pro-Collagen 1 alpha 1 is a component of the extracellular matrix (ECM).

Other ELISA kits to measure the ECM and its role in tissue function include:

- Human Fibronectin ELISA kit ab219046
- Human MMP1 ELISA kit ab215083
- Human Elastin ELISA kit ab239433

The antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.8" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 94 %", "average": "= 93" }, { "sample": "Cell culture media", "range": "97 - 101 %", "average": "= 99" }, { "sample": "Citrate plasma", "range": "102 - 110 %", "average": "= 106" }, { "sample": "EDTA Plasma", "range": "105 - 114 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "94 - 107 %", "average": "= 101" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).
See full target information COL1A1
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Publications (53)

Recent publications for all applications. Explore the full list and refine your search

Pharmaceutics 17: PubMed39861784

2025

Biotechnological Phytocomplex of (L.) DC. Enhances Collagen Biosynthesis In Vitro and Improves Skin Elasticity In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Rigillo,Giovanna Pressi,Oriana Bertaiola,Chiara Guarnerio,Matilde Merlin,Roberto Zambonin,Stefano Pandolfo,Angela Golosio,Francesca Masin,Fabio Tascedda,Marco Biagi,Giulia Baini

Physiological reports 12:e16136 PubMed39501714

2024

Evaluating the antifibrotic potential of naringenin, asiatic acid, and icariin using murine and human precision-cut liver slices.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Luo,Yana Geng,Dorenda Oosterhuis,Vincent E de Meijer,Peter Olinga

Cells 13: PubMed39404414

2024

Kisspeptin Alleviates Human Hepatic Fibrogenesis by Inhibiting TGFβ Signaling in Hepatic Stellate Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kavita Prasad,Dipankar Bhattacharya,Shams Gamal Eldin Shams,Kimberly Izarraras,Tia Hart,Brent Mayfield,Maryjka B Blaszczyk,Zhongren Zhou,Utpal B Pajvani,Scott L Friedman,Moshmi Bhattacharya

International journal of molecular sciences 25: PubMed39125720

2024

Analysis of Active Components and Transcriptome of Callus Extract and Its Effects against Oxidative Stress and Wrinkles in Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Euihyun Kim,Morgane De Tollenaere,Benedicte Sennelier,Carole Lambert,Anais Durduret,Soo-Yun Kim,Hyo-Hyun Seo,Jung-Hun Lee,Amandine Scandolera,Romain Reynaud,Sang-Hyun Moh

Cells 13: PubMed38994938

2024

Fibrogenesis in Human Mucosa and Muscularis Precision-Cut Intestinal Slices.

Applications

Unspecified application

Species

Unspecified reactive species

Carin Biel,Anniek Kastermans,Janneke Heidema,Martin Pehrsson,Charlotte Henstra,Joachim Mortensen,Klaas Nico Faber,Peter Olinga

JCI insight 9: PubMed38780544

2024

Methionine as a regulator of bone remodeling with fasting.

Applications

Unspecified application

Species

Unspecified reactive species

Tânia Amorim,Naveen Gv Kumar,Natalie L David,William Dion,Trishya Pagadala,Nandini K Doshi,Bokai Zhu,Andrey Parkhitko,Matthew L Steinhauser,Pouneh K Fazeli

JCI insight 9: PubMed38564292

2024

Role of the mitochondrial protein cyclophilin D in skin wound healing and collagen secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Bansal,Monica Torres,Matthew Hunt,Nuoqi Wang,Margarita Chatzopoulou,Mansi Manchanda,Evan P Taddeo,Cynthia Shu,Orian S Shirihai,Etty Bachar-Wikstrom,Jakob D Wikstrom

Nutrients 16: PubMed38474754

2024

Metabolic Dysfunction-Associated Steatotic Liver Disease in a Dish: Human Precision-Cut Liver Slices as a Platform for Drug Screening and Interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Li,Frederik T Larsen,Marius C van den Heuvel,Konstanze Gier,Alan R Gorter,Dorenda Oosterhuis,Johan Bijzet,Vincent E de Meijer,Kim Ravnskjaer,Anika Nagelkerke,Peter Olinga

Yonsei medical journal 65:98-107 PubMed38288650

2024

Exploring the Safety and Efficacy of Organic Light-Emitting Diode in Skin Rejuvenation and Wound Healing.

Applications

Unspecified application

Species

Unspecified reactive species

Young In Lee,Sang Gyu Lee,Seoyoon Ham,Inhee Jung,Jangmi Suk,Ju Hee Lee

Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 32:135-145 PubMed38243615

2024

The effects of TGF-β1 and IFN-α2b on decorin, decorin isoforms and type I collagen in hypertrophic scar dermal fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth E Eremenko,Peter O Kwan,Jie Ding,Sunita Ghosh,Edward E Tredget
View all publications
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Product promise

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