Human Pro-Collagen I alpha 1 ELISA Kit ab210966 is a single-wash 90-min SimpleStep ELISA® used to quantify Human Pro-Collagen I alpha 1 with a sensitivity of 5.3 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human Pro-Collagen I alpha 1 sandwich ELISA Kit:
Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
Colorimetric assay - 450nm readout; works on any plate reader
Looking for more flexibility? We also offer antibody pairs ready for conjugation
Images
Publications
- Scientific reports 13:179692023The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexe Grenier,Mathieu C Morissette,Patrick J Rochette,Roxane PouliotPubMed 37863977
- Journal of experimental orthopaedics 10:842023Minoxidil weakens newly synthesized collagen in fibrotic synoviocytes from osteoarthritis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Sarkovich,Peter P Issa,Andrew Longanecker,Davis Martin,Kaitlyn Redondo,Patrick McTernan,Jennifer Simkin,Luis MarreroPubMed 37605092
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
39.06 - 2500 pg/mL
- Assay time
1h 30m
- Sensitivity
= 5.3 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
What's included?
Recommended products
Human Pro-Collagen I alpha 1 ELISA Kit ab210966 is a single-wash 90-min SimpleStep ELISA® used to quantify Human Pro-Collagen I alpha 1 with a sensitivity of 5.3 pg/ml. The assay uses a simple Mix-Wash-Read protocol with just one incubation and wash step.
Easy-to-use, rapid, sensitive Human Pro-Collagen I alpha 1 sandwich ELISA Kit:
Mix, Wash, Read: get results in 90 minutes with SimpleStep ELISA® format
Colorimetric assay - 450nm readout; works on any plate reader
Looking for more flexibility? We also offer antibody pairs ready for conjugation
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Key facts
- Detection method
Colorimetric
- Sample types
Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
Sandwich (quantitative)
- Reactive species
Human
- Range
39.06 - 2500 pg/mL
- Assay time
1h 30m
- Assay Platform
Pre-coated microplate (12 x 8 well strips)
- Sensitivity
= 5.3 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 1.8 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 91 - 94 % |
Sample type Cell culture media | Average % = 99 | Range 97 - 101 % |
Sample type Citrate plasma | Average % = 106 | Range 102 - 110 % |
Sample type EDTA Plasma | Average % = 108 | Range 105 - 114 % |
Sample type Heparin Plasma | Average % = 101 | Range 94 - 107 % |
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
+4°C
Notes
Pro-Collagen I alpha 1 ELISA kit (ab210966) is designed for the quantitative measurement of human Pro-Collagen I alpha 1 / Pro-Collagen I N-Terminal Propeptide (PINP) in serum, plasma, cell culture supernatants, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pro-Collagen I alpha 1 with 5.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.
SPECIES REACTIVITY This kit recognizes human Pro-Collagen I alpha 1 protein. Other species reactivity was determined by measuring 1:100 (dilution) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
The antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- Activity summary
Collagen I also known as collagen type 1 is a protein that provides mechanical support to tissues. It forms large fibrils and has a molecular mass of about 300 kilodaltons. Collagen I is expressed in connective tissues including skin bones tendons and ligaments. It serves as a structural scaffold that imparts tensile strength and stability. This protein is essential for maintaining the integrity of extracellular matrices in these tissues.
- Biological function summary
Collagen I plays a central role in tissue architecture and wound healing. It is part of a larger collagen family comprising fibers that form a network within the extracellular space. In addition to its structural functions collagen I interacts with cells influencing processes like cell adhesion and migration. It undergoes remodeling through enzymes such as collagenases during tissue repair. Collagen I engages with various matrix proteins reinforcing its function in maintaining tissue homeostasis.
- Pathways
Collagen I is involved in signaling pathways related to extracellular matrix organization and remodeling. Its interactions within the transforming growth factor-beta (TGF-?) pathway highlight its role in regulating tissue repair and fibrosis. Within this pathway collagen I associates with other proteins like fibronectin. In this context matrix metalloproteinases also relate to collagen I as they modify its structure and influence matrix dynamics.
- Associated diseases and disorders
Collagen I changes associate strongly with fibrosis and osteogenesis imperfecta. In fibrosis excessive collagen I accumulation leads to scarring and tissue function impairment. Proteins like TGF-? influence abnormal collagen I deposition in this context. In osteogenesis imperfecta mutations in collagen I genes disrupt its function affecting bone development and strength. Osteocalcin another bone-associated protein interacts with collagen I contributing to skeletal matrix formation.
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14 product images
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Raw value data for example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Raw data values are shown in the table.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentrations of native Pro-Collagen I alpha 1 in human serum and plasma samples.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 142.1 ng/mL in serum, 135.9 ng/mL in plasma (citrate), 112.1 ng/mL in plasma (EDTA) and 102.1 ng/mL in plasma (heparin).
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentrations of native Pro-Collagen I alpha 1 in human IMR-90 extract based on a 2 μg/mL extract load.
The concentrations of Pro‑Collagen I alpha 1 were measured in duplicate and interpolated from the Pro‑Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1.62 ng/mL in IMR-90 extract.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Serum from ten individual healthy human female donors was diluted 1:200 and measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 197.3 ng/mL with a range of 113.0 – 417 ng/mL.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Linearity of dilution - native human Pro-Collagen I alpha 1 in human serum, plasma (citrate, EDTA, Heparin) and IMR-90 Extract.
Native human Pro-Collagen I alpha 1 was measured in human serum, plasma, and IMR-90 lysate in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for the IMR-90 lysate.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Linearity of dilution - recombinant human Pro-Collagen I alpha in cell culture media.
Recombinant human Pro-Collagen I alpha 1 was spiked into cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2 µg/mL IMR-90 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate.
Background-subtracted data values (mean +/- SD) are graphed.
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