Human Pro-Collagen I alpha 1 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human pro-collagen I alpha 1 with a sensitivity of 5.3 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, plasma, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 40 publications
Images
Publications
Filter by
- Scientific reports 13:179692023The combination of cigarette smoke and solar rays causes effects similar to skin aging in a bilayer skin model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexe Grenier,Mathieu C Morissette,Patrick J Rochette,Roxane PouliotPubMed 37863977
- Journal of experimental orthopaedics 10:842023Minoxidil weakens newly synthesized collagen in fibrotic synoviocytes from osteoarthritis patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Sarkovich,Peter P Issa,Andrew Longanecker,Davis Martin,Kaitlyn Redondo,Patrick McTernan,Jennifer Simkin,Luis MarreroPubMed 37605092
- Molecular medicine reports 28:2023Polydeoxyribonucleotide exerts opposing effects on ERK activity in human skin keratinocytes and fibroblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun Mee Shin,Eun Joo Baek,Kwang Ho Kim,Kwang Joong Kim,Eun Joo ParkPubMed 37350391
- Journal of clinical medicine 12:2023Topical Application of Peptide Nucleic Acid Antisense Oligonucleotide for MMP-1 and Its Potential Anti-Aging Properties.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoung In Lee,Sang Gyu Lee,Inhee Jung,Jangmi Suk,Chaemin Baeg,Seon-Young Han,Jeong Yeon Seo,Daram Jung,Yeasel Jeon,Ju Hee LeePubMed 37048555
- JBMR plus 7:e107032023Impact of Alcohol on Bone Health in People Living With HIV: Integrating Clinical Data From Serum Bone Markers With Morphometric Analysis in a Non-Human Primate Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra Denys,Allison Norman,Daniel S Perrien,Larry J Suva,Liz Simon,Lee S McDaniel,Tekeda Ferguson,Kim Pedersen,David Welsh,Patricia E Molina,Martin Jj RonisPubMed 36699637
- Cell reports 42:1120282023SAYSD1 senses UFMylated ribosome to safeguard co-translational protein translocation at the endoplasmic reticulum.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLihui Wang,Yue Xu,Sijung Yun,Quan Yuan,Prasanna Satpute-Krishnan,Yihong YePubMed 36848233
- Pharmaceutics 15:2023A Novel (L.) Britton Cell-Derived Phytocomplex Regulates Keratinocytes Inflammatory Cascade and Barrier Function and Preserves Vaginal Mucosal Integrity In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGiovanna Pressi,Giovanna Rigillo,Paolo Governa,Vittoria Borgonetti,Giulia Baini,Raffaella Rizzi,Chiara Guarnerio,Oriana Bertaiola,Marco Frigo,Matilde Merlin,Stefania Paltrinieri,Roberto Zambonin,Stefano Pandolfo,Marco BiagiPubMed 36678869
- Communications biology 5:14012022Gut microbiota modulates lung fibrosis severity following acute lung injury in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOzioma S Chioma,Elizabeth K Mallott,Austin Chapman,Joseph C Van Amburg,Hongmei Wu,Binal Shah-Gandhi,Nandita Dey,Marina E Kirkland,M Blanca Piazuelo,Joyce Johnson,Gordon R Bernard,Sobha R Bodduluri,Steven Davison,Bodduluri Haribabu,Seth R Bordenstein,Wonder P DrakePubMed 36543914
- Science immunology 7:eabq66912022Epiregulin is a dendritic cell-derived EGFR ligand that maintains skin and lung fibrosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIan D Odell,Holly Steach,Stephen B Gauld,Lauren Reinke-Breen,Jozsef Karman,Tracy L Carr,Joseph B Wetter,Lucy Phillips,Monique Hinchcliff,Richard A FlavellPubMed 36490328
- International journal of implant dentistry 8:392022Plasma rich in growth factors (PRGF) and leukocyte-platelet rich fibrin (L-PRF): comparative release of growth factors and biological effect on osteoblasts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura Baca-Gonzalez,Rebeca Serrano Zamora,Lisa Rancan,Francisco González Fernández-Tresguerres,Isabel Fernández-Tresguerres,Rosa M López-Pintor,Juan López-Quiles,Isabel Leco,Jesús TorresPubMed 36184700
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.06 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 5.3 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
What's included?
Recommended products
Human Pro-Collagen I alpha 1 ELISA kit is a single-wash, 90-minute SimpleStep ELISA® used to quantify human pro-collagen I alpha 1 with a sensitivity of 5.3 pg/mL. The assay employs a simple Mix-Wash-Read protocol with just one incubation and wash step.
- Colorimetric sandwich ELISA – 450 nm readout, compatible with any standard plate reader
- Available in different formats – 384-well format available for high-throughput applications
- Broad sample compatibility – works with serum, plasma, cell culture extracts, tissue extracts, and cell culture supernatant
- Cited in over 40 publications
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39.06 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 5.3 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 1.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 91 - 94 % |
Sample type Cell culture media | Average % = 99 | Range 97 - 101 % |
Sample type Citrate plasma | Average % = 106 | Range 102 - 110 % |
Sample type EDTA Plasma | Average % = 108 | Range 105 - 114 % |
Sample type Heparin Plasma | Average % = 101 | Range 94 - 107 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Pro-Collagen I alpha 1 ELISA Kit ab210966 is a rapid single-wash 90-min ELISA kit to measure Human Pro-Collagen I alpha 1 in cell culture extracts, tissue extracts, cell culture supernatant, serum and plasma. This SimpleStep ELISA® sensitivity is 5.3 pg/mL.
How the assay works
Human Pro-Collagen I alpha 1 SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
This kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.
Human Pro-Collagen I alpha 1 ELISA Kit ab210966 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm
How other researchers are using Human Pro-Collagen I alpha 1/ COL1A1 ELISA kit ab210966
Human Pro-Collagen I alpha 1/ COL1A1 ELISA kit ab210966 has been used to study cartilage regeneration and osteoarthritis treatment(1)and the effects of cigarette smoke and the sun on skin aging(2).
References:
(1)Kerong Dai et al. 2022, PMID: 36114225,
(2)Roxane Pouliot et al. 2023, PMID: 37863977
Save your precious samples
Our 384-well Human Pro-Collagen I alpha 1 ELISA Kit only requires a maxmum of 12.5µL
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free Human Pro-Collagen I alpha 1 Antibody Pair - BSA and Azide free ab241825
- Anti-Procollagen Type 1 antibody [EPR19126-161] - BSA and Azide free (Capture) Anti-Procollagen Type 1 antibody [EPR19126-161] - BSA and Azide free (Capture) ab242540
- Anti-Procollagen Type 1 antibody [EPR19126-152] - BSA and Azide free (Detector) Anti-Procollagen Type 1 antibody [EPR19126-152] - BSA and Azide free (Detector) ab242788
Related and recommended products
Human Pro-Collagen I alpha 1/COL1A1 ELISA kit ab210966 is commonly used to study tissue structure and function. Human Pro-Collagen 1 alpha 1 is a component of the extracellular matrix (ECM).
Other ELISA kits to measure the ECM and its role in tissue function include:
- Human Fibronectin ELISA kit Human Fibronectin ELISA Kit ab219046
- Human MMP1 ELISA kit Human MMP1 ELISA Kit ab215083
- Human Elastin ELISA kit Human Elastin ELISA Kit ab239433
The antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
14 product images
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Raw value data for example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Raw data values are shown in the table.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentrations of native Pro-Collagen I alpha 1 in human serum and plasma samples.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 142.1 ng/mL in serum, 135.9 ng/mL in plasma (citrate), 112.1 ng/mL in plasma (EDTA) and 102.1 ng/mL in plasma (heparin).
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentrations of native Pro-Collagen I alpha 1 in human IMR-90 extract based on a 2 μg/mL extract load.
The concentrations of Pro‑Collagen I alpha 1 were measured in duplicate and interpolated from the Pro‑Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1.62 ng/mL in IMR-90 extract.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Serum from ten individual healthy human female donors was diluted 1:200 and measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 197.3 ng/mL with a range of 113.0 – 417 ng/mL.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Linearity of dilution - native human Pro-Collagen I alpha 1 in human serum, plasma (citrate, EDTA, Heparin) and IMR-90 Extract.
Native human Pro-Collagen I alpha 1 was measured in human serum, plasma, and IMR-90 lysate in a 2-fold dilution series. Sample dilutions are made in Sample Diluent NS for serum and plasma. Sample dilutions are made in Sample Diluent 1X Cell Extraction Buffer PTR for the IMR-90 lysate.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Linearity of dilution - recombinant human Pro-Collagen I alpha in cell culture media.
Recombinant human Pro-Collagen I alpha 1 was spiked into cell culture media and diluted in a 2-fold dilution series in Sample Diluent NS.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1% human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Interpolated concentration of native Pro-Collagen I alpha 1 was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 2 µg/mL IMR-90 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit (ab210966)
Example of human Pro-Collagen I alpha 1 standard curve in 96-well vs. 384-well plate.
Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com