Skip to main content

Human Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Pro-Collagen I alpha 1 in Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum samples.

This product has no reviews yet! Submit a review
Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Sensitivity

= 5.3 pg/mL

Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Application
sELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

Function

Type I collagen is a member of group I collagen (fibrillar forming collagen).

What's included?

1 x 96 Tests
Components
10X Human Pro-Collagen I alpha 1 Capture Antibody
1 x 600 µL
10X Human Pro-Collagen I alpha 1 Detector Antibody
1 x 600 µL
10X Wash Buffer PT (ab206977)
1 x 20 mL
50X Cell Extraction Enhancer Solution (ab193971)
1 x 1 mL
5X Cell Extraction Buffer PTR (ab193970)
1 x 10 mL
Antibody Diluent CPI2
1 x 6 mL
Human Pro-Collagen I alpha 1 Lyophilized Recombinant Protein
2 x 1 Vial
Plate Seals
1 x 1 Unit
Sample Diluent NS (ab193972)
1 x 50 mL
SimpleStep Pre-Coated 96-Well Microplate (ab206978)
1 x 1 Unit
Stop Solution
1 x 12 mL
TMB Development Solution
1 x 12 mL

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Human Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Pro-Collagen I alpha 1 in Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum samples.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum

Assay type

Sandwich (quantitative)

Reactive species

Human

Range

39.06 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

Sensitivity

= 5.3 pg/mL

Precision

Intra assay

Sample
Serum
n
8
mean
-
SD
-
C.V.
1.8

Inter assay

Sample
Serum
n
3
mean
-
SD
-
C.V.
3

Recovery

Sample specific recovery

Sample type
Serum
Average %
= 93
Range
91 - 94 %
Sample type
Cell culture media
Average %
= 99
Range
97 - 101 %
Sample type
Citrate plasma
Average %
= 106
Range
102 - 110 %
Sample type
EDTA Plasma
Average %
= 108
Range
105 - 114 %
Sample type
Heparin Plasma
Average %
= 101
Range
94 - 107 %

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

Notes

Pro-Collagen I alpha 1 ELISA kit (ab210966) is designed for the quantitative measurement of human Pro-Collagen I alpha 1 / Pro-Collagen I N-Terminal Propeptide (PINP) in serum, plasma, cell culture supernatants, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pro-Collagen I alpha 1 with 5.3 pg/mL sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.

ASSAY SPECIFICITY This kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.

SPECIES REACTIVITY This kit recognizes human Pro-Collagen I alpha 1 protein. Other species reactivity was determined by measuring 1:100 (dilution) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow

The antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com