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Human Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Pro-Collagen I alpha 1 in Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum samples.
Alternative names=Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Colorimetric
Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
39.06 - 2500 pg/mL
1h 30m
= 5.3 pg/mL
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Human Pro-Collagen I alpha 1 ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Pro-Collagen I alpha 1 in Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum samples.
Alternative names=Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
Colorimetric
Plasma, Cell culture extracts, Tissue Extracts, Cell culture supernatant, Serum
Sandwich (quantitative)
Human
39.06 - 2500 pg/mL
1h 30m
Pre-coated microplate (12 x 8 well strips)
= 5.3 pg/mL
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 1.8 |
Sample | n | mean | SD | C.V. |
---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 3 |
Sample type | Average % | Range |
---|---|---|
Sample type Serum | Average % = 93 | Range 91 - 94 % |
Sample type Cell culture media | Average % = 99 | Range 97 - 101 % |
Sample type Citrate plasma | Average % = 106 | Range 102 - 110 % |
Sample type EDTA Plasma | Average % = 108 | Range 105 - 114 % |
Sample type Heparin Plasma | Average % = 101 | Range 94 - 107 % |
Blue Ice
+4°C
+4°C
Pro-Collagen I alpha 1 ELISA kit (ab210966) is designed for the quantitative measurement of human Pro-Collagen I alpha 1 / Pro-Collagen I N-Terminal Propeptide (PINP) in serum, plasma, cell culture supernatants, and cell and tissue extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Pro-Collagen I alpha 1 with 5.3 pg/mL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
ASSAY SPECIFICITY This kit recognizes both native and recombinant human Pro-Collagen I alpha 1 protein in serum, plasma, cell culture supernatant, and cell and tissue extract samples only.
SPECIES REACTIVITY This kit recognizes human Pro-Collagen I alpha 1 protein. Other species reactivity was determined by measuring 1:100 (dilution) serum samples of various species, interpolating the protein concentrations from the human standard curve, and expressing the interpolated concentrations as a percentage of the protein concentration in human serum assayed at the same dilution.
Reactivity < 3% was determined for the following species: Mouse, Rat, Cow
The antibodies in this kit are generated against Pro-Collagen I N-Terminal Propeptide (PINP). Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
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