Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Pro-Collagen I alpha 1 with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Images
Publications
Filter by
- BioFactors (Oxford, England) 49:1038-10602023Skincare potential of a sustainable postbiotic extract produced through sugarcane straw fermentation by Saccharomyces cerevisiae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarco Duarte,Maria João Carvalho,Nelson Mota de Carvalho,João Azevedo-Silva,Adélia Mendes,Inês Pinto Ribeiro,João Carlos Fernandes,Ana L S Oliveira,Carla Oliveira,Manuela Pintado,Ana Amaro,Ana Raquel MadureiraPubMed 37317790
- Experimental and therapeutic medicine 19:2353-23592020Gremlin mediates the TGF-β-induced induction of profibrogenic genes in human retinal pigment epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Qin et. al.PubMed 32104303
- International journal of molecular sciences 20:2019Biomimetic Synthesis of Nanocrystalline Hydroxyapatite Composites: Therapeutic Potential and Effects on Bone Regeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChih-Hsiang Fang et. al.PubMed 31795225
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.91 - 16000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.7 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Type I collagen is a member of group I collagen (fibrillar forming collagen).
Alternative names
Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1
What's included?
Recommended products
Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent is a single-wash 90-min Simplestep used to quantify Human Pro-Collagen I alpha 1 with a sensitivity of 3.7 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Fluorescent Sandwich ELISA - 530/570/590 nm readout : works on any standard plate reader
Key facts
- Detection method
- Fluorescent
- Sample types
- Cell culture extracts, Tissue Extracts, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 3.91 - 16000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.7 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 1.8 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 93 | Range 91 - 94 % |
Sample type EDTA Plasma | Average % = 108 | Range 105 - 114 % |
Sample type Heparin Plasma | Average % = 101 | Range 94 - 107 % |
Sample type Citrate plasma | Average % = 106 | Range 102 - 110 % |
Sample type Cell culture media | Average % = 99 | Range 97 - 101 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Pro-Collagen I alpha 1 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Pro-Collagen I alpha 1 protein in human serum, plasma, cell culture supernatants, and cell and tissue extract samples.
This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
If using a Molecular Devices' plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.
The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.
Type I collagen is the most abundant structural protein of connective tissues such as skin, bone and tendon. It is synthesized as a pro-collagen molecule that is characterized by a 300 nm triple helical domain flanked by globular N- and C-terminal propeptides. Specifically, human Pro-Collagen I alpha 1 consists of a signal peptide (amino acids (aa) 1-22), a propeptide (aa 23-161), the mature chain (aa 162-1218), and another propeptide (aa 1219 – 1464). The non-helical propeptides are removed by procollagen N- and C-proteinase activities so that the mature triple helices can self-assemble into collagen fibrils that provide tensile strength to tissues.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Procollagen Type 1 also known as Pro-1 collagen is a precursor molecule for collagen Type I which is the most abundant protein in the human body. Its molecular mass is approximately 230 kDa. This protein is synthesized by fibroblasts and is widely expressed in connective tissues including skin tendons and bones. Once synthesized procollagen Type 1 undergoes post-translational modifications and is cleaved by specific enzymes to form mature collagen fibers contributing to the structural integrity of the extracellular matrix.
- Biological function summary
The synthesis and processing of procollagen Type 1 play a major role in maintaining tissue strength and elasticity. This molecule is not part of a larger complex but undergoes self-assembly to form the rigid triple-helical structure typical of collagen fibers. These fibers are essential for providing mechanical support to tissues and facilitating cell adhesion. Additionally its presence is often required for normal wound healing processes and tissue regeneration.
- Pathways
The secretion and assembly of procollagen Type 1 occur through the secretory pathway. Transforming growth factor-beta (TGF-beta) signaling significantly regulates this process influencing fibrogenesis. This pathway also involves the interaction with proteins such as fibronectin and integrins which are important for aligning collagen fibers within the extracellular matrix. Furthermore procollagen Type 1 engages in the Wnt signaling pathway affecting the arrangement and stability of collagen networks.
- Associated diseases and disorders
Mutations or dysregulation of procollagen Type 1 synthesis may contribute to conditions such as osteogenesis imperfecta and scleroderma. In osteogenesis imperfecta defective collagen leads to brittle bones due to improper fiber formation. In scleroderma an overproduction of collagen causes skin thickening and organ fibrosis. The altered interaction of procollagen Type 1 with proteins like lysyl oxidase can exacerbate these pathological conditions highlighting the significance of proper collagen processing and assembly for tissue health.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
ELISA - Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent (ab229389)
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent (ab229389)
Example of human Pro-Collagen I alpha 1 standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent (ab229389)
Interpolated concentrations of native Pro-Collagen I alpha 1 in human serum and plasma samples.
The concentrations of Pro-Collagen I alpha 1 were measured in duplicates, interpolated from the Pro-Collagen I alpha 1 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1%, plasma (citrate) 1%, plasma (EDTA) 1%, and plasma (heparin) 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 142.1 ng/mL in serum, 135.9 ng/mL in plasma (citrate), 112.1 ng/mL in plasma (EDTA) and 102.1 ng/mL in plasma (heparin).
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent (ab229389)
Interpolated concentrations of native Pro-Collagen I alpha 1 in human IMR-90 extract based on a 2 μg/mL extract load.
The concentrations of Pro Collagen I alpha 1 were measured in duplicate and interpolated from the Pro Collagen I alpha 1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 1.62 ng/mL in IMR-90 extract.
Sandwich ELISA - Human Pro-Collagen I alpha 1 ELISA Kit, Fluorescent (ab229389)
Serum from ten individual healthy human female donors was diluted 1:200 and measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Pro-Collagen I alpha 1 concentration was determined to be 197.3 ng/mL with a range of 113.0 – 417 ng/mL.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com