AB272198

Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP)

Recombinant
SimpleStep
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Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) is a single-wash 90-min Simplestep used to quantify Human Pro-Collagen I C-Terminal Propeptide (PICP) with a sensitivity of 8.15 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Collagen alpha-1(I) chain, Alpha-1 type I collagen, COL1A1

5 Images

ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

ELISA

Supplier Data

ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Interpolated concentrations of native Pro-Collagen I C-Terminal Propeptide in human skin tissue extract sample based on a 50 μg/mL extract load

sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Example of human Pro-Collagen I C-Terminal Propeptide standard curve in Sample Diluent NS

sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Serum from ten individual healthy human female donors was measured in duplicate

sELISA

Supplier Data

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Interpolated concentrations of native Pro-Collagen I C-Terminal Propeptide in human serum and plasma samples

Key facts

Detection method

Colorimetric

Sample types

Cell culture media, Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.15 pg/mL

Range

23.44 - 1500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) ab272198 is a rapid single-wash 90-min Sandwich ELISA to measure Human Pro-Collagen I C-Terminal Propeptide (PICP)in cell culture media, citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 8.15 pg/mL.

How the assay works

Human Pro-Collagen I C-Terminal Propeptide (PICP)SimpleStep ELISA^{^®}employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^{^®} plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^{^®} protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) ab272198 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PICP antibody [EPR22894-16] - BSA and Azide free (Capture) ab272379
- Anti-PICP antibody [EPR22209-103] - BSA and Azide free (Detector) ab272379

Pro-Collagen I C-Terminal Propeptide (PICP), encoded by the COL1A1 gene, is the fibrillar collagen that serves as a major component of type I collagen found in cartilage and most connective tissues. Studies have shown that mutations in the mutations in the Pro-Collagen I C-Terminal Propeptide are associated with osteoporosis, osteogenesis imperfecta diseases, and Ehlers-Danlos syndrome. The standard protein in this product is the C‑terminal Propeptide region of Collagen alpha-1(I).

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.1" } ]

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Recovery

[ { "sample": "Serum", "range": "92 - 109 %", "average": "= 102" }, { "sample": "EDTA Plasma", "range": "85 - 100 %", "average": "= 89" }, { "sample": "Cell culture media", "range": "103 - 109 %", "average": "= 107" }, { "sample": "Heparin Plasma", "range": "85 - 92 %", "average": "= 89" }, { "sample": "Citrate plasma", "range": "80 - 106 %", "average": "= 90" } ]

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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab272198-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB272198-CMP05", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB272198-CMP12", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB272198-CMP11", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Pro-Collagen I C-Terminal Propeptide (PICP) Lyophilized Recombinant Protein", "number":"AB272198-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Capture Antibody", "number":"AB272198-CMP08", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB272198-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB272198-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB272198-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Pro-Collagen I C-Terminal Propeptide (PICP) Detector Antibody", "number":"AB272198-CMP09", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB272198-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB272198-CMP04", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PICP also known as procollagen type 1 C-peptide or collagen C-peptide is a byproduct released during the synthesis of type I collagen. Type I collagen is the most abundant collagen type in the human body providing structure to skin bone tendons and other connective tissues. PICP has a molecular mass of approximately 35-38 kDa. It is expressed in tissues where type I collagen is synthesized including bone and skin fibroblasts.

Biological function summary

PICP serves as a marker for collagen biosynthesis closely reflecting the rate of collagen type I production. PICP does not form part of a larger complex but exists as a separate entity once cleaved from procollagen. Its presence in the bloodstream allows researchers to monitor collagen turnover which is vital in understanding tissue formation and remodeling processes.

Pathways

PICP connects with collagen synthesis and deposition pathways particularly relevant in bone formation and skin repair. It interacts indirectly with proteins like collagenase during collagen degradation and remodeling. Increased PICP levels indicate heightened collagen synthesis correlating with anabolic processes especially in bone metabolism pathways.

PICP levels are significant in bone-related conditions like osteoporosis where decreased collagen synthesis is often observed. It also correlates with fibrotic conditions including liver fibrosis where elevated collagen production marks disease progression. In these contexts PICP relates to other proteins such as procollagen type III N-peptide (PIIINP) which also serve as markers of pathological collagen deposition.

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Product protocols

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Target data

Type I collagen is a member of group I collagen (fibrillar forming collagen).

See full target information COL1A1

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websiteProtocolBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP)

ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Sandwich ELISA - Human Pro-Collagen I C-Terminal Propeptide ELISA Kit (PICP) (AB272198)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Assay Kits

Product promise