Human Pro-GRP (31-98) ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human Pro-GRP (31-98) with a sensitivity of 3.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.469 - 350 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 3.99 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Stimulates the release of gastrin and other gastrointestinal hormones (By similarity). Contributes to the perception of prurient stimuli and to the transmission of itch signals in the spinal cord that promote scratching behavior (By similarity). Contributes primarily to nonhistaminergic itch sensation (By similarity). In one study, shown to act in the amygdala as part of an inhibitory network which inhibits memory specifically related to learned fear (By similarity). In another study, shown to act on vasoactive intestinal peptide (VIP)-expressing cells in the auditory cortex, most likely via extrasynaptic diffusion from local and long-range sources, to mediate disinhibition of glutamatergic cells via VIP cell-specific GRPR signaling which leads to enhanced auditory fear memories (By similarity). Contributes to the regulation of food intake (By similarity). Inhibits voltage-gated sodium channels but enhances voltage-gated potassium channels in hippocampal neurons (By similarity). Induces sighing by acting directly on the pre-Botzinger complex, a cluster of several thousand neurons in the ventrolateral medulla responsible for inspiration during respiratory activity (By similarity). Neuromedin-C. Induces an itch response through activation of receptors present on mast cells, triggering mast cell degranulation.
Alternative names
Gastrin-releasing peptide, GRP
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Human Pro-GRP (31-98) ELISA Kit- extracellular is a single-wash 90-min Simplestep used to quantify Human Pro-GRP (31-98) with a sensitivity of 3.99 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
- Detection method
- Colorimetric
- Sample types
- Serum, EDTA Plasma, Citrate plasma, Heparin Plasma, Cerebral Spinal Fluid, Cell culture supernatant
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 5.469 - 350 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 3.99 pg/mL
- General recovery
- 97%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. = 5.5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. = 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 97 | Range 98 - 102 % |
Sample type EDTA Plasma | Average % = 97 | Range 94 - 101 % |
Sample type Citrate plasma | Average % = 84 | Range 78 - 95 % |
Sample type Heparin Plasma | Average % = 86 | Range 79 - 98 % |
Sample type Cerebral Spinal Fluid | Average % = 96 | Range 89 - 106 % |
Sample type Cell culture supernatant | Average % = 95 | Range 94 - 97 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human Pro-GRP (31-98) SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Pro-GRP (31-98) protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human Pro-GRP (31-98) with 3.99 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab315065) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Pro-GRP (31-98) also known as Progastrin-releasing peptide is a peptide with a mass of approximately 8 kDa. It serves as a precursor for gastrin-releasing peptide (GRP). It undergoes cleavage to form the active GRP peptide. GRP belongs to the bombesin-like peptide family and you can primarily find it expressed in neuroendocrine tissues particularly in the gastrointestinal tract and lungs. This peptide plays an important role in various cellular signaling processes by binding to its receptors.
- Biological function summary
The gastrin-releasing peptide influences the release of important hormones such as gastrin. It acts on the gastrointestinal system contributing to the stimulation of gastric acid secretion. Additionally GRP has a role in neurotransmission. It forms part of a signaling system that operates in neuroendocrine communication where it works with other neuropeptides to regulate physiological processes.
- Pathways
The gastrin-releasing peptide impacts several signaling pathways including the gastrin secretion pathway and the bombesin receptor pathway. It interacts with receptors on target cells to induce cellular responses including growth and secretory functions. Related proteins like the bombesin receptor subtype 3 are involved in these pathways modulating different biochemical reactions that contribute to digestion and central nervous system signaling.
- Associated diseases and disorders
Gastrin-releasing peptide has associations with small cell lung cancer and gastrointestinal disorders. Elevated levels of Pro-GRP (31-98) can serve as a biomarker for small cell lung cancer aiding in diagnosis and monitoring treatment responses. The interplay of GRP with the bombesin receptor and related pathways like those involving gastrin underlines its importance in certain tumorigenic processes and dysregulation in gastrointestinal functions.
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2 product images
Sandwich ELISA - Human Pro-GRP (31-98) ELISA Kit- extracellular (ab315304)
Interpolated concentrations of native Pro-GRP (31-98) was measured in duplicate at different sample concentrations. Undiluted samples are 50% CSF and 50% female individual serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
Sandwich ELISA - Human Pro-GRP (31-98) ELISA Kit- extracellular (ab315304)
Example of human Pro-GRP (31-98) standard curve. Background-subtracted data values (mean +/- SD) are graphed.
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Product protocols
- Visit the general protocols
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