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AB100630

Human Procalcitonin ELISA Kit

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(9 Publications)

Human Procalcitonin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Procalcitonin in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CALC1, CALCA, Calcitonin

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Sandwich ELISA - Human Procalcitonin ELISA Kit (AB100630)
  • sELISA

Supplier Data

Sandwich ELISA - Human Procalcitonin ELISA Kit (AB100630)

Representative Standard Curve using ab100630.

Sandwich ELISA - Human Procalcitonin ELISA Kit (AB100630)
  • sELISA

Supplier Data

Sandwich ELISA - Human Procalcitonin ELISA Kit (AB100630)

Representative Standard Curve using ab100630.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

< 20 pg/mL

Range

27.43 - 20000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Procalcitonin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Procalcitonin in plasma and cell culture supernatants. (Human Procalcitonin concentration is pretty low in normal plasma, it may not be detected in this assay).

This assay employs an antibody specific for Human Procalcitonin coated on a 96-well plate. Standards and samples are pipetted into the wells and Procalcitonin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Procalcitonin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Procalcitonin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

We have not been able to detect endogenous Human Procalcitonin in normal serum with ab100630, only in serum spiked with Human Procalcitonin.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "80 - 111 %", "average": "= 100.5" }, { "sample": "Plasma", "range": "65 - 88 %", "average": "= 72.35" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100630-1x96Tests", "assetComponentDetails": [ { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100630-CMP02", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100630-CMP09", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100630-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human Procalcitonin", "number":"AB100630-CMP01", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100630-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100630-CMP10", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent D", "number":"AB100630-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Procalcitonin Microplate (12 x 8 wells)", "number":"AB100630-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human Procalcitonin Standard (lyophilized)", "number":"AB100630-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"700X HRP-Streptavidin Concentrate", "number":"AB100630-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Procalcitonin also known as PCT is a protein precursor of the hormone calcitonin. It has a molecular mass of about 13 kilodaltons. Procalcitonin is expressed in the thyroid gland typically but can also be produced in other tissues like the liver and kidney during inflammation or sepsis. It gets increasingly produced in response to bacterial infections making it a significant marker for conditions involving systemic inflammation.
Biological function summary

The function of procalcitonin is related to its precursor status for calcitonin which helps regulate calcium homeostasis. During infections PCT levels rise without its cleavage into active calcitonin indicating that its biological role extends beyond calcium regulation. It does not form part of any larger protein complex in its marker role. When released in response to bacterial infections procalcitonin serves as an indicator for the severity of the infection which is particularly useful in distinguishing bacterial from viral infections.

Pathways

Procalcitonin gets involved in the immune response pathway primarily due to its expression during bacterial infections. An important pathway is the inflammatory response where procalcitonin production gets triggered serving as a mediator in immune system activation. It is related to cytokines in the immune response which elevated PCT can represent indirectly indicating the presence and level of infection-driven inflammation.

Procalcitonin levels are used significantly in diagnosing and managing sepsis and bacterial infections. Procalcitonin concentrations rise sharply in a bacterial systemic infection and help differentiate it from viral infections an important diagnostic criterion. Additionally in sepsis another important protein involved is C-reactive protein (CRP) often used alongside PCT as a biomarker for inflammatory states in the body. This relationship underlines the importance of PCT in managing infectious diseases through targeted diagnostic pathways.
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Product protocols

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Target data

Calcitonin. Calcitonin is a peptide hormone that causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Calcitonin function is mediated by the calcitonin receptor/CALCR and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed : 35324283).. Katacalcin. Katacalcin is a potent plasma calcium-lowering peptide.
See full target information CALCA
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

The journal of extra-corporeal technology 57:96-104 PubMed40523137

2025

The utility of sTREM-1 and presepsin to predict infection in pediatric patients receiving mechanical circulatory support.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Murray,Jianli Bi,Robin Alexander,Md Rejuan Haque,Brian Beckman,Ruth Seabrook,W Joshua Frazier,Andrew R Yates

Hepatology communications 8: PubMed38180983

2024

A randomized study of ceftriaxone for the prevention of infections in hospitalized patients with advanced cirrhosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zachary Fricker,Gordon Jiang,Het Patel,Annabel McLaughlin,Sofia Izunza Barba,Sebastian Niezen,Michael Curry

Experimental and therapeutic medicine 23:403 PubMed35637648

2022

Circulating miR-15b, Annexin A1, procalcitonin and interleukin-6 levels differentiate children with metabolically unhealthy obesity from those with metabolically healthy obesity: A case-control study.

Applications

Unspecified application

Species

Unspecified reactive species

Khalid M Mohany,Osamah Al Rugaie,Osama Al-Wutayd,Mansour Alsharidah,Abdullah Al-Nafeesah

Microbiology spectrum 9:e0191021 PubMed34937173

2021

Recombinant Fasciola hepatica Fatty Acid Binding Protein as a Novel Anti-Inflammatory Biotherapeutic Drug in an Acute Gram-Negative Nonhuman Primate Sepsis Model.

Applications

Unspecified application

Species

Unspecified reactive species

Jose J Rosado-Franco,Albersy Armina-Rodriguez,Nicole Marzan-Rivera,Armando G Burgos,Natalie Spiliopoulos,Stephanie M Dorta-Estremera,Loyda B Mendez,A M Espino

Animal models and experimental medicine 2:326-333 PubMed31942564

2019

Outlining key inflammation-associated parameters during early phase of an experimental gram-negative sepsis model in rhesus macaques ().

Applications

Unspecified application

Species

Unspecified reactive species

Jose J Rosado-Franco,Marcos J Ramos-Benitez,Laura M Parodi,Derick Rosario,Nicole Compo,Luis D Giavedoni,Ana M Espino

Experimental and therapeutic medicine 16:1493-1498 PubMed30116399

2018

Adjuvant rhubarb alleviates organs dysfunction and inhibits inflammation in heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wan,Shuang-Shuang Sun,Hai-Yan Fu,Yin-Kun Xu,Qing Liu,Jiang-Tao Yin,Bing Wan

Diabetic medicine : a journal of the British Diabetic Association 35:255-261 PubMed28734103

2017

Levels of wound calprotectin and other inflammatory biomarkers aid in deciding which patients with a diabetic foot ulcer need antibiotic therapy (INDUCE study).

Applications

Unspecified application

Species

Unspecified reactive species

J R Ingram,S Cawley,E Coulman,C Gregory,E Thomas-Jones,T Pickles,R Cannings-John,N A Francis,K Harding,K Hood,V Piguet

Experimental and therapeutic medicine 14:855-861 PubMed28673010

2017

Rhubarb vs. glycerin enema for treatment of critically ill patients with intra-abdominal hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Wan,Hao Zhang,Jiangtao Yin,Haiyan Fu,Yikun Chen,Liping Yang,Dadong Liu,Tangfeng Lv,Yong Song

PLoS medicine 10:e1001577; discussion e1001577 PubMed24391478

2013

Circulating mitochondrial DNA in patients in the ICU as a marker of mortality: derivation and validation.

Applications

Unspecified application

Species

Unspecified reactive species

Kiichi Nakahira,Sun-Young Kyung,Angela J Rogers,Lee Gazourian,Sojung Youn,Anthony F Massaro,Carolina Quintana,Juan C Osorio,Zhaoxi Wang,Yang Zhao,Laurie A Lawler,Jason D Christie,Nuala J Meyer,Finnian R Mc Causland,Sushrut S Waikar,Aaron B Waxman,Raymond T Chung,Raphael Bueno,Ivan O Rosas,Laura E Fredenburgh,Rebecca M Baron,David C Christiani,Gary M Hunninghake,Augustine M K Choi
View all publications
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