Human Procalcitonin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Procalcitonin with a sensitivity of 1.51 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
View Alternative Names
CALC1, CALCA, Calcitonin
- sELISA
Supplier Data
Sandwich ELISA - Human Procalcitonin ELISA Kit (AB221828)
Interpolated concentrations of native Procalcitonin in human serum and plasma samples.
The concentrations of Procalcitonin were measured in duplicates, interpolated from the Procalcitonin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration was determined to be 54 pg/mL in neat serum, 38 pg/mL in neat plasma (citrate), 114 pg/mL in neat plasma (EDTA) and 43 pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Procalcitonin ELISA Kit (AB221828)
Interpolated concentrations of native Procalcitonin in human urine, milk, and spiked Procalcitonin in human saliva samples.
The concentrations of Procalcitonin were measured in duplicates, interpolated from the Procalcitonin standard curves and corrected for sample dilution. Undiluted samples are as follows : urine 25%, milk 50%, and saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration was determined to be 1,555 pg/mL in neat urine, 154 pg/mL in neat milk, and 338 pg/mL in neat saliva.
- sELISA
Supplier Data
Sandwich ELISA - Human Procalcitonin ELISA Kit (AB221828)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration in neat serum was determined to be 139 pg/mL with a range of 33 – 438 pg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human Procalcitonin ELISA Kit (AB221828)
Interpolated concentrations of spiked Procalcitonin in human serum and plasma samples.
The concentrations of Procalcitonin were measured in duplicates, interpolated from the Procalcitonin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration was determined to be 335 pg/mL in neat serum, 351 pg/mL in neat plasma (citrate), 424 pg/mL in neat plasma (EDTA) and 405 pg/mL in neat plasma (heparin).
- sELISA
Supplier Data
Sandwich ELISA - Human Procalcitonin ELISA Kit (AB221828)
Example of human Procalcitonin standard curve.
Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human Procalcitonin ELISA Kit (ab221828) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Procalcitonin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Procalcitonin with 1.51 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Human Procalcitonin is a 116-amino acid precursor protein of the peptide hormone called Calcitonin coded by the gene CALCA. Procalcitonin is mainly produced by the C-cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, Procalcitonin is cleaved into three specific fragments: an N-terminal residue, Calcitonin, and Katacalcin. Levels of unprocessed Procalcitonin have been shown to rise significantly after bacterial infection, trauma, and shock. Calcitonin regulates the blood phosphate and calcium levels by promoting incorporation into bones. Populations with chronic kidney disease have been shown to have higher procalcitonin levels. The antibodies used in this product were raised to the propeptide sequence residues 26-82. The standard protein in this product has been mass calibrated to a full-length procalcitonin (residues 26-141).
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Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of procalcitonin is related to its precursor status for calcitonin which helps regulate calcium homeostasis. During infections PCT levels rise without its cleavage into active calcitonin indicating that its biological role extends beyond calcium regulation. It does not form part of any larger protein complex in its marker role. When released in response to bacterial infections procalcitonin serves as an indicator for the severity of the infection which is particularly useful in distinguishing bacterial from viral infections.
Pathways
Procalcitonin gets involved in the immune response pathway primarily due to its expression during bacterial infections. An important pathway is the inflammatory response where procalcitonin production gets triggered serving as a mediator in immune system activation. It is related to cytokines in the immune response which elevated PCT can represent indirectly indicating the presence and level of infection-driven inflammation.
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Cirugia y cirujanos 90:632-637 PubMed36327468
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Nature 587:460-465 PubMed33149301
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International journal of reproductive biomedicine 16:623-628 PubMed30643854
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Emergency (Tehran, Iran) 6:e51 PubMed30584567
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