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Human Progesterone ELISA Kit is a Competitive ELISA kit for the measurement of Human Progesterone in Human in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.


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ELISA - Human Progesterone ELISA Kit (AB108670), expandable thumbnail

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Key facts

Detection method
Colorimetric
Sample types
Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
Assay type
Competitive
Reactive species
Human
Range
0.2 - 40 ng/mL
Sensitivity
= 0.05 ng/mL

Reactivity data

Application
cELISA
Reactivity
Reacts
Dilution info
-
Notes

-

Associated Products

Select an associated product type

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Target data

Alternative names

Pregn 4 ene 3 20 dione

What's included?

1 x 96 Tests
Components
10X Wash Solution
1 x 50 mL
Anti-Progesterone IgG Coated Wells
1 x 1 Unit
Cover foils
1 x 1 Unit
Progesterone Standard 0 - 0 ng/mL
1 x 1 mL
Progesterone Standard 1 - 0.2 ng/mL
1 x 1 mL
Progesterone Standard 2 - 1.0 ng/mL
1 x 1 mL
Progesterone Standard 3 - 5.0 ng/mL
1 x 1 mL
Progesterone Standard 4 - 15.0 ng/mL
1 x 1 mL
Progesterone Standard 5 - 40.0 ng/mL
1 x 1 mL
Progesterone control
1 x 1 mL
Progesterone-HRP Conjugate
1 x 22 mL
Stop Solution
1 x 15 mL
Strip holder
1 x 1 Unit
TMB Substrate Solution
1 x 15 mL

Recommended products

Human Progesterone ELISA Kit is a Competitive ELISA kit for the measurement of Human Progesterone in Human in Citrate plasma, EDTA Plasma, Heparin Plasma, Serum samples.

Key facts

Detection method
Colorimetric
Sample types
Citrate plasma, EDTA Plasma, Heparin Plasma, Serum
Assay type
Competitive
Reactive species
Human
Range
0.2 - 40 ng/mL
Assay Platform
Microplate
Sensitivity
= 0.05 ng/mL
General recovery
106%

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C

Notes

Abcam's Progesterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Progesterone in serum and plasma.

A 96-well plate has been precoated with anti-Progesterone. Samples and the Progesterone-HRP conjugate are added to the wells, where Progesterone in the sample competes with the added Progesterone-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Progesterone in the sample and the intensity is measured at 450 nm.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Progesterone also called P4 is a steroid hormone playing an important role in the reproductive system. Its molecular weight is approximately 314.47 Da. This hormone is mainly expressed in the ovaries but also in the adrenal glands and placenta. Progesterone acts by binding to its receptor the progesterone receptor (PR) which is found in target tissues such as the uterus mammary glands and the brain. When progesterone binds to PR it translocates to the nucleus to act as a transcription factor regulating gene expression that is important for reproductive function.

Biological function summary

Progesterone is essential for preparation and maintenance of pregnancy. It facilitates the transformation of the endometrium to a secretory phase to support embryo implantation. It also modulates immune responses during pregnancy to prevent the maternal immune system from rejecting the fetus. Progesterone and its receptor are not part of a larger protein complex but they do interact with other molecular pathways to exert their actions. Additionally progesterone inhibits the proliferation of mammary gland epithelial cells contributing to breast development and function.

Pathways

Progesterone plays a significant role in the menstrual cycle and pregnancy-related pathways. It works within the ovarian steroidogenesis pathway which includes other hormones like estrogen and luteinizing hormone. This pathway ensures the release and regulation of female sex hormones. Progesterone also interacts with the MAPK signaling pathway which involves proteins such as ERK1/2 affecting cellular processes including cell growth and differentiation.

Associated diseases and disorders

Progesterone is linked to conditions such as endometriosis and breast cancer. In endometriosis progesterone resistance leads to the abnormal growth of endometrial tissue outside the uterus. The hormone's association with breast cancer involves its interaction with proteins like estrogen receptor alpha which can promote cancer cell proliferation when dysregulated. Understanding progesterone’s role in disease helps in developing therapeutic strategies to manage these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • ELISA - Human Progesterone ELISA Kit (ab108670), expandable thumbnail

    ELISA - Human Progesterone ELISA Kit (ab108670)

    Representative Standard Curve using ab108670.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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