AB252364

Human Progranulin ELISA Kit

Recombinant
SimpleStep
What is this?

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(2 Publications)

Human Progranulin ELISA Kit is a single-wash 90-min SimpleStep Sandwich ELISA used to quantify human progranulin with a sensitivity of 72.68 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric sandwich ELISA - 450 nm readout, works on any plate reader
- Different formats for different needs: 10x96-well plates for bulk orders and 384-well for higher throughput
- Validated on a number of sample types including cerebrospinal fluid (CSF)
- Need to design your own assay: we also offer the conjugation-ready antibody pair

View Alternative Names

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN

8 Images

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

sELISA

Lab

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

sELISA

Lab

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Interpolated concentration of native Progranulin was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 5% human serum. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Human Progranulin Standard Curve Comparison.

Standard Curve comparison between Human Progranulin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Interpolated concentrations of native Progranulin in human milk, saliva, and cell culture supernatant samples.

The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 160, saliva 5%, and OVCAR-3 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 360.2 ng/ml in milk, 60.4 ng/ml in saliva, and 24.4 ng/ml in OVCAR-3 supernatant.

sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Interpolated concentrations of native Progranulin in human serum and plasma samples.

The concentrations of Progranulin were measured in duplicates, interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5%, plasma (citrate) 6.25%, plasma (heparin) 6.25%, and plasma (EDTA) 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 60.9 ng/mL in serum, 47.8 ng/mL in plasma (citrate), 52.2 ng/ml in plasma (heparin), and 49.8 ng/ml in plasma (EDTA).

sELISA

Lab

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Example of human Progranulin standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Example of human Progranulin standard curve in Sample Diluent NS.

The Progranulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Progranulin concentration was determined to be 67.7 ng/mL with a range of 43.7 – 124.8 ng/mL.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Milk, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 72.68 pg/mL

Range

156.25 - 10000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Progranulin ELISA Kit ab252364 is a rapid single-wash 90-min ELISA kit to measure Human Progranulin in cerebral spinal fluid (CSF), saliva, urine, milk, serum and plasma. This SimpleStep ELISA® sensitivity is 5.64 pg/mL.

How the assay works
Human Progranulin SimpleStep ELISA® employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity
This kit is designed for the quantification of human Progranulin. The standard protein in this kit is mature full-length human Progranulin. Native signal was detected in serum, plasma (heparin), plasma (EDTA), plasma (citrate), cell culture supernatant, milk, saliva, and CSF samples. Spiked protein experiments were used to validate urine and CSF sample types. Cell extract and tissue extract samples have not been tested with this kit.
25% male and female urine samples from healthy donors were measured in duplicate. All values were below the detectable range of the assay.

Human Progranulin ELISA Kit ab252364 protocol summary:
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate for 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add stop solution
5. Read the results on a plate reader at 450nm

How other researchers are using Human Progranulin ELISA Kit ab252364
Human Progranulin ELISA Kit ab252364 has been used to develop a prognostic test for uveal melanoma ⁽¹⁾.

References:
⁽¹⁾Gustav Stalhammar et al. 2023, PMID: 36973672

Save your precious samples
Our 384-well Human Progranulin ELISA Kit only requires a maxmum of 12.5µL

Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation
- Human Progranulin Antibody Pair - BSA and Azide free ab256708
- Anti-Progranulin antibody [EPR22207-103] - BSA and Azide free (Capture) ab259663
- Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) ab259664

Related and recommended products
Human Progranulin ELISA Kit ab252364 is commonly used to study inflammation, wound repair, and neurodegeneration.

Other products to measure neuronal cell health include:
- Human Glutathione Peroxidase 4 ELISA kit ab304936
- Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] ab125066
- Anti-MAP2 antibody [EPR22641-16] ab254264

To measure progranulin in mice:
- Mouse Progranulin ELISA kit ab213473

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Precision

[ { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" }, { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" } ]

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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "103 - 121 %", "average": "= 112" }, { "sample": "Cell culture supernatant", "range": "92 - 98 %", "average": "= 95" }, { "sample": "Milk", "range": "88 - 108 %", "average": "= 98" }, { "sample": "Serum", "range": "95 - 106 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "99 - 114 %", "average": "= 107" }, { "sample": "Urine", "range": "86 - 91 %", "average": "= 89" }, { "sample": "Saliva", "range": "90 - 99 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "114 - 123 %", "average": "= 119" }, { "sample": "Citrate plasma", "range": "97 - 110 %", "average": "= 106" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.

Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.

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Product protocols

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Target data

The protein expressed by the GRN gene is a secreted regulator of lysosomal function and a growth factor involved in processes such as inflammation, wound healing, and cell proliferation. It regulates protein trafficking to lysosomes and lysosomal enzyme activity, facilitating lysosomal acidification which leads to the degradation of mature CTSD by CTSB. It also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation, thus preventing the release of oxidants and proteases. Additionally, it modulates inflammation in neurons by preserving neuron survival, axonal outgrowth, and neuronal integrity. Granulin-3, -4, and -7 have distinct roles : Granulin-4 promotes proliferation of epithelial cell line A431 in culture; Granulin-3 inhibits epithelial cell proliferation and induces IL-8 secretion; and Granulin-7 stabilizes CTSD by maintaining its aspartic-type peptidase activity through interaction. This supplementary information is collated from multiple sources and compiled automatically.

See full target information GRN

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 299:105475 PubMed37981208

2023

Antisense oligonucleotides targeting the miR-29b binding site in the GRN mRNA increase progranulin translation.

Applications

Unspecified application

Species

Unspecified reactive species

Geetika Aggarwal,Subhashis Banerjee,Spencer A Jones,Yousri Benchaar,Jasmine Bélanger,Myriam Sévigny,Denise M Smith,Michael L Niehoff,Monica Pavlack,Ian Mitchelle S de Vera,Terri L Petkau,Blair R Leavitt,Karen Ling,Paymaan Jafar-Nejad,Frank Rigo,John E Morley,Susan A Farr,Paul A Dutchak,Chantelle F Sephton,Andrew D Nguyen

BMC cancer 23:277 PubMed36973672

2023

A serum protein signature at the time of Uveal Melanoma diagnosis predicts long-term patient survival.

Applications

Unspecified application

Species

Unspecified reactive species

Christina Herrspiegel,Flavia Plastino,Emma Lardner,Stefan Seregard,Pete A Williams,Helder André,Gustav Stålhammar

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Progranulin ELISA Kit

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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