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AB327046

Human Progranulin ELISA Kit, Chemiluminescent

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Human Progranulin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Progranulin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 54.87 - 40000 pg/ml with a sensitivity of 28.49 pg/mL.
- Different formats for different needs: 10x 96-well plates for bulk orders
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending
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Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)
  • sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)

Interpolated concentrations of native Progranulin in human milk saliva and cell culture supernatant samples.

The concentrations of Progranulin were measured in duplicates interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows : milk 1 : 160 saliva 5% and OVCAR-3 supernatant 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Progranulin concentration was determined to be 360.2 ng/ml in milk 60.4 ng/ml in saliva and 24.4 ng/ml in OVCAR-3 supernatant.

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)
  • sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)

Serum from ten individual healthy human female donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Progranulin concentration was determined to be 67.7 ng/mL with a range of 43.7 – 124.8 ng/mL.

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)
  • sELISA

Unknown

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)

Interpolated concentrations of native Progranulin in human serum and plasma samples.

The concentrations of Progranulin were measured in duplicates interpolated from the Progranulin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 5% plasma (citrate) 6.25% plasma (heparin) 6.25% and plasma (EDTA) 6.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Progranulin concentration was determined to be 60.9 ng/mL in serum 47.8 ng/mL in plasma (citrate) 52.2 ng/ml in plasma (heparin) and 49.8 ng/ml in plasma (EDTA).

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)
  • sELISA

Lab

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)

Standard curve comparison between the Chemiluminescent Human Progranulin SimpleStep ELISA and colorimetric Human Progranulin SimpleStep ELISA (colorimetric ab252364) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)
  • sELISA

Lab

Sandwich ELISA - Human Progranulin ELISA Kit, Chemiluminescent (AB327046)

Dynamic range comparison between the Chemiluminescent Human Progranulin SimpleStep ELISA, colorimetric Human Progranulin SimpleStep ELISA (colorimetric ab252364), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Key facts

Detection method

Luminescent

Sample types

Cerebral Spinal Fluid, Cell culture supernatant, Milk, Serum, EDTA Plasma, Urine, Saliva, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 28.49 pg/mL

Range

54.87 - 40000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Progranulin ELISA Kit, Chemiluminescent (ab327046) is a rapid single-wash sandwich 55-min ELISA to measure Human Progranulin in Cerebral Spinal Fluid, Cell culture supernatant, Milk, Serum, EDTA Plasma, Urine, Saliva, Heparin Plasma, Citrate plasma with a sensitivity of 28.49 pg/mL.

How the assay works

Human Progranulin ELISA Kit, Chemiluminescent (ab327046) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Progranulin (ab327046) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Progranulin antibody [EPR22207-103] - BSA and Azide free (Capture) ab259663
- Anti-Progranulin antibody [EPR22103-42] - BSA and Azide free (Detector) ab259664
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Precision

[ { "reproducibilityType": "Intra", "sample": "Plasma", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.7" }, { "reproducibilityType": "Inter", "sample": "Plasma", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.5" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "103 - 121 %", "average": "= 112" }, { "sample": "Cell culture supernatant", "range": "92 - 98 %", "average": "= 95" }, { "sample": "Milk", "range": "88 - 108 %", "average": "= 98" }, { "sample": "Serum", "range": "95 - 106 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "99 - 114 %", "average": "= 107" }, { "sample": "Urine", "range": "86 - 91 %", "average": "= 89" }, { "sample": "Saliva", "range": "90 - 99 %", "average": "= 94" }, { "sample": "Heparin Plasma", "range": "114 - 123 %", "average": "= 119" }, { "sample": "Citrate plasma", "range": "97 - 110 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12526812, PubMed : 18378771, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286). Regulates protein trafficking to lysosomes, and also the activity of lysosomal enzymes (PubMed : 28453791, PubMed : 28541286). Also facilitates the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (By similarity). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 12526812). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 18378771).. Granulin-4. Promotes proliferation of the epithelial cell line A431 in culture.. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
See full target information GRN

Alternative Names

Progranulin, PGRN, Acrogranin, Epithelin precursor, Glycoprotein of 88 Kda, Granulin precursor, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, GRN
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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