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AB242235

Human Proinsulin ELISA Kit

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(2 Publications)

Human Proinsulin ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Proinsulin with a sensitivity of 0.75 pmol/L. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Insulin, INS

3 Images
Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)
  • sELISA

Unknown

Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)

Serum from ten individual healthy human female donors, nine individual healthy human male donors, and three individual male donors with type 2 diabetes were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Only those samples that showed detectable signal are shown above.

Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)
  • sELISA

Unknown

Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)

Example of human Proinsulin standard curve in Sample Diluent NS.

The Proinsulin standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)
  • sELISA

Unknown

Sandwich ELISA - Human Proinsulin ELISA Kit (AB242235)

Human Proinsulin Standard Curve Comparison. Standard Curve comparison between human Proinsulin SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Key facts

Detection method

Colorimetric

Sample types

Heparin Plasma, Citrate plasma, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.75 pmol/L

Range

4.99 - 319.5 pmol/L

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Proinsulin ELISA Kit (ab242235) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Proinsulin protein in cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Proinsulin with 7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

Proinsulin is the precursor of insulin, an essential and primary hormone involved in glucose metabolism. Proinsulin is synthesized in the beta cells of the islets of Langerhans in the pancreas, where it is processed in the ER into active, mature insulin. Active, mature insulin is stored in secretory granules. Rising blood glucose levels lead to the release of active, mature insulin into the blood stream. Proinsulin levels in the blood are elevated with age, in type II diabetes, and during beta cell dysregulation.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" }, { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.2" } ]
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Recovery

[ { "sample": "Serum", "range": "102 - 114 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "102 - 116 %", "average": "= 108" }, { "sample": "Heparin Plasma", "range": "95 - 104 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "92 - 102 %", "average": "= 95" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab242235-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB242235-CMP07", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB242235-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Proinsulin Lyophilized Recombinant Protein", "number":"AB242235-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB242235-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB242235-CMP11", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Proinsulin Capture Antibody", "number":"AB242235-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB242235-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB242235-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human Proinsulin Detector Antibody", "number":"AB242235-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB242235-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Insulin often referred to as 'insulin' is a peptide hormone with a mass of approximately 5.8 kDa generated by the beta cells in the pancreas. Its precursor proinsulin is synthesized as a larger molecule with a mass of about 9 kDa. Both insulin and proinsulin are primarily expressed in the pancreatic islets of Langerhans. Proinsulin undergoes enzymatic cleavage to form mature insulin and C-peptide. Insulin plays an essential role in glucose metabolism by regulating cellular uptake of glucose.
Biological function summary

Insulin and proinsulin are indispensable for maintaining glucose homeostasis in the body. Insulin binds to its receptor a receptor tyrosine kinase on target cells like muscle and adipose tissue to facilitate glucose uptake. Proinsulin also has a lesser-known role in insulin regulation and folding. It mostly exists independently rather than being part of a complex with insulin but precursor and mature insulin forms can transiently appear together during synthesis and secretion.

Pathways

Insulin function fits significantly into the signaling pathways such as the insulin signaling pathway and the PI3K/AKT pathway. These pathways importantly involve glucose transporters like GLUT4 and proteins such as IRS-1 that mediate insulin's effects on glucose and lipid metabolism. Insulin signals lead to the translocation of GLUT4 to the plasma membrane enhancing glucose uptake. Therefore insulin is closely related to these critical metabolic pathways.

Dysfunctions in insulin and proinsulin levels or actions are linked to conditions like diabetes mellitus and metabolic syndrome. In diabetes insulin resistance or deficiency leads to uncontrolled blood glucose levels highlighting the importance of insulin in glucose regulation. The link between insulin and obesity often involves dysregulation in insulin signaling pathways contributing to metabolic complications. Understanding these processes and their diseases is vital for developing therapeutic strategies.
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Product protocols

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information Insulin
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 13:22436 PubMed38105338

2023

Serum proinsulin levels as peripheral blood biomarkers in patients with cognitive impairment.

Applications

Unspecified application

Species

Unspecified reactive species

Abhinbhen W Saraya,Chavit Tunvirachaisakul,Chanikarn Sonpee,Panticha Katasrila,Tanyares Sathaporn,Supatporn Tepmongkol,Sookjareon Tangwongchai

Endocrine reviews 42:528-583 PubMed34180979

2021

The β Cell in Diabetes: Integrating Biomarkers With Functional Measures.

Applications

Unspecified application

Species

Unspecified reactive species

Steven E Kahn,Yi-Chun Chen,Nathalie Esser,Austin J Taylor,Daniël H van Raalte,Sakeneh Zraika,C Bruce Verchere
View all publications
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Product promise

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