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AB326745

Human Proinsulin ELISA Kit, Chemiluminescent

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Human Proinsulin ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Proinsulin with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 0.76 - 1672.05 pmol/l with a sensitivity of 0.19 pmol/L.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
- Patent pending

View Alternative Names

Insulin, INS

3 Images
Sandwich ELISA - Human Proinsulin ELISA Kit, Chemiluminescent (AB326745)
  • sELISA

Lab

Sandwich ELISA - Human Proinsulin ELISA Kit, Chemiluminescent (AB326745)

Standard curve comparison between the Chemiluminescent Human Proinsulin SimpleStep ELISA and colorimetric Human Proinsulin SimpleStep ELISA (ab242235) in Sample Diluent NS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Proinsulin ELISA Kit, Chemiluminescent (AB326745)
  • sELISA

Lab

Sandwich ELISA - Human Proinsulin ELISA Kit, Chemiluminescent (AB326745)

Dynamic range comparison between the Chemiluminescent Human Proinsulin SimpleStep ELISA, colorimetric Human Proinsulin SimpleStep ELISA (ab242235), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Proinsulin ELISA Kit, Chemiluminescent (AB326745)
  • sELISA

Unknown

Sandwich ELISA - Human Proinsulin ELISA Kit, Chemiluminescent (AB326745)

Serum from ten individual healthy human female donors, nine individual healthy human male donors, and three individual male donors with type 2 diabetes were measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Only those samples that showed detectable signal are shown above.

Key facts

Detection method

Luminescent

Sample types

Serum, EDTA Plasma, Heparin Plasma, Citrate plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.19 pmol/L

Range

0.76 - 1672.05 pmol/L

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Proinsulin ELISA Kit, Chemiluminescent (ab326745) is a rapid single-wash sandwich 55-min ELISA to measure Human Proinsulin in Serum, EDTA Plasma, Heparin Plasma, Citrate plasma with a sensitivity of 0.19 pmol/L.

How the assay works

Human Proinsulin ELISA Kit, Chemiluminescent (ab326745) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Proinsulin (ab326745) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-Insulin(25-110*2) antibody [EPR20432-54] - BSA and Aside free (Capture) ab244829
- Anti-Insulin(25-110*2) antibody [EPR20432-228] - BSA and Aside free (Detector) ab244849
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Precision

[ { "reproducibilityType": "Intra", "sample": "Protein", "replicates": 5, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.2" }, { "reproducibilityType": "Inter", "sample": "Protein", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 5.6" } ]
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Recovery

[ { "sample": "Serum", "range": "102 - 114 %", "average": "= 114" }, { "sample": "EDTA Plasma", "range": "102 - 116 %", "average": "= 116" }, { "sample": "Heparin Plasma", "range": "95 - 104 %", "average": "= 104" }, { "sample": "Citrate plasma", "range": "92 - 102 %", "average": "= 102" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab326745-1x96Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human Proinsulin Detector Antibody", "number":"AB326745-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"10X Human Proinsulin Capture Antibody", "number":"AB326745-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB326745-CMP10", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent A", "number":"AB326745-CMP07", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB326745-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB326745-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated Black 96-Well Microplate", "number":"AB326745-CMP09", "productcode":"" }, { "size":"1 x 3 mL", "name":"ChemiHRP Reagent B", "number":"AB326745-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human Proinsulin Lyophilized Recombinant Protein", "number":"AB326745-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPI2", "number":"AB326745-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
See full target information Insulin
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com