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AB108679

Human Prolactin ELISA Kit

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(1 Publication)

Human Prolactin ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Prolactin in Human in Biofluids samples.

View Alternative Names

Prolactin, PRL

Key facts

Detection method

Colorimetric

Sample types

Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.25 ng/mL

Range

5 - 100 ng/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Prolactin in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Prolactin in Human serum.

Microtiterstrip wells are precoated with inactivated specific Corynebacterium diphtheriae toxin (toxoid) antigens to bind corresponding antibodies of the specimen. After washing the wells to remove all unbound sample material horseradish peroxidase (HRP) labelled anti-human IgG conjugate is added. This conjugate binds to the captured C. diphtheriae toxin-specific antibodies. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is proportional to the amount of C. diphtheriae toxin-specific IgG antibodies in the specimen. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450 nm is read using an ELISA microwell plate reader.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Sample 1", "replicates": 20, "mean": "6.58 %", "standardDeviation": "0.45", "coefficientOfVariability": "6.77" }, { "reproducibilityType": "Inter", "sample": "Sample 2", "replicates": 20, "mean": "21.34 %", "standardDeviation": "1.3", "coefficientOfVariability": "6.11" }, { "reproducibilityType": "Inter", "sample": "Sample 3", "replicates": 20, "mean": "58.39 %", "standardDeviation": "2.93", "coefficientOfVariability": "5.01" }, { "reproducibilityType": "Intra", "sample": "Sample 1", "replicates": 20, "mean": "6.32 %", "standardDeviation": "0.4", "coefficientOfVariability": "6.3" }, { "reproducibilityType": "Intra", "sample": "Sample 2", "replicates": 20, "mean": "19.22 %", "standardDeviation": "1.19", "coefficientOfVariability": "6.18" }, { "reproducibilityType": "Intra", "sample": "Sample 3", "replicates": 20, "mean": "54.52 %", "standardDeviation": "2.64", "coefficientOfVariability": "4.84" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prolactin also known as PRL is a protein hormone with a molecular mass of approximately 23 kDa. It is primarily produced in the anterior pituitary gland but is also found in other tissues such as the placenta mammary glands and immune cells. Mechanically Prolactin functions as a peptide hormone and exerts its effects by binding to the Prolactin receptor a member of the cytokine receptor superfamily. This binding activates the Jak-STAT signaling pathway which is important for mediating the hormone's effects in target cells.
Biological function summary

The functions of this hormone extend beyond its well-known role in lactation. It helps stimulate milk production in the mammary glands by promoting the proliferation and differentiation of mammary epithelial cells. Additionally Prolactin influences reproductive functions metabolism and immune regulation. It is not part of a larger protein complex but interacts with various proteins to perform its functions. Notably it modulates the immune response contributing to tolerance during pregnancy and affecting the balance between pro-inflammatory and anti-inflammatory cytokines.

Pathways

Prolactin plays important roles in the Jak-STAT and MAPK signaling pathways. In these pathways it shares interactions with related proteins such as Growth Hormone and Prolactin Receptor aiding in cell growth differentiation and metabolism regulation. Prolactin's involvement in these pathways highlights its central role in physiological processes linking various hormonal signals to regulate body functions efficiently.

Prolactin's imbalance can lead to conditions such as hyperprolactinemia and prolactinomas both characterized by excessive levels of this hormone in the blood. These conditions are often related to alterations in the pituitary gland function. Prolactin interacts closely with Dopamine a neurotransmitter that inhibits its secretion influencing both its production and release. Dysregulation of this interaction may contribute to the development of these disorders impacting endocrine health and overall well-being.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Prolactin acts primarily on the mammary gland by promoting lactation.
See full target information PRL
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in endocrinology 14:1026168 PubMed36967761

2023

Bromocriptine inhibits proliferation in the endometrium from women with adenomyosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yiqun Tang,Sakthivignesh Ponandai-Srinivasan,Caroline Frisendahl,Johanna K Andersson,Dora Pavone,Elizabeth A Stewart,Parameswaran Grace Luther Lalitkumar,Eberhard Korsching,Nageswara Rao Bogavarappu,Kristina Gemzell-Danielsson
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

Select an associated product type
Alternative Product
Assay Kits

AB226901

Human Prolactin/PRL ELISA Kit

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