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AB326392

Human Prolactin/PRL ELISA Kit, Chemiluminescent

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Human Prolactin/PRL ELISA Kit, Chemiluminescent is a 55-min single-wash SimpleStep Ignite™ ELISA for the rapid quantification of Human Prolactin/PRL with higher sensitivity and broader measuring range.

- Single 30 min incubation and single-wash step for results in less than an hour.
- Chemiluminescent detection allows a broad dynamic range: 8.23 - 18000 pg/ml with a sensitivity of 3.5pg/mL.
- Design your own immunoassay: we also offer the conjugation-ready antibody pair.
-Patent pending

View Alternative Names

Decidual prolactin, GHA1, Growth hormone A1, Lactogenic Hormone, Luteotropic Hormone, Mammotropin, Prolactin, Prolactin precursor

6 Images
Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)
  • sELISA

Lab

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)

SimpleStep ELISA® Ignite technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 55 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)
  • sELISA

Lab

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)

Dynamic range comparison between the Chemiluminescent Human Prolactin SimpleStep ELISA, colorimetric Human Prolactin SimpleStep ELISA (ab226901), and leading colorimetric competitor. The Chemiluminescent SimpleStep ELISA kit shows increased sensitivity and increased dynamic range.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)
  • sELISA

Lab

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)

Standard curve comparison between the Chemiluminescent Human Prolactin SimpleStep ELISA and colorimetric Human Prolactin SimpleStep ELISA (ab226901) in Sample Diluent 25BS. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)

Interpolated concentrations of native Prolactin in human pregnant serum and milk.

The concentrations of Prolactin were measured in duplicate and interpolated from the Prolactin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Prolactin concentration was determined to be 42647 pg/mL in pregnant serum and 1214 pg/mL in milk.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)

Serum from ten individual healthy human female donors and ten individual healthy male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Prolactin concentration in females was determined to be 2778 pg/mL with a range of 294 – 18132 pg/mL. The mean Prolactin concentration in males was determined to be 1733 pg/mL with a range of 179 – 7626 pg/mL.

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)
  • sELISA

Supplier Data

Sandwich ELISA - Human Prolactin/PRL ELISA Kit, Chemiluminescent (AB326392)

Interpolated concentrations of native Prolactin in human serum and plasma and of spiked Prolactin in cell culture media samples.

The concentrations of Prolactin were measured in duplicates interpolated from the Prolactin standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 12.5% plasma (citrate) 100% plasma (heparin) 100% plasma (EDTA) 100% and RPMI media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD n=2). The mean Prolactin concentration was determined to be 1066 pg/mL in serum 480 pg/mL in plasma (citrate) 801 pg/mL in plasma (heparin) and 711 pg/mL in plasma (EDTA).

Key facts

Detection method

Luminescent

Sample types

Milk, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 3.5 pg/mL

Range

8.23 - 18000 pg/mL

Assay time

55m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

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Product details

Human Prolactin/PRL ELISA Kit, Chemiluminescent (ab326392) is a rapid single-wash sandwich 55-min ELISA to measure Human Prolactin/PRL in Milk, Serum, EDTA Plasma, Heparin Plasma, Citrate plasma, Cell culture media with a sensitivity of 3.5 pg/mL.

How the assay works

Human Prolactin/PRL ELISA Kit, Chemiluminescent (ab326392) SimpleStep Ignite™ ELISA employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step during a 30 min only incubation, significantly reducing assay time. By utilizing a chemiluminscent detection method this improves the sensitivity and dynamic range when compared to traditional colorimetric ELISAs.

Assay Specificity

Our SimpleStep Ignite™ ELISA kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human Prolactin/PRL (ab326392) protocol summary

1. Mix : add samples / standards to the wells together with the capture and detector antibody cocktail. Incubate 30 min at room temperature
2. Wash
3. Add ChemiHRP development solution - incubate for 2 min
4. Read the results on a plate reader capable of luminescence detection

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-PRL antibody [EPR20580-116] - BSA and Aside free (Capture) ab244682
- Anti-PRL antibody [EPR20580-114] - BSA and Aside free (Detector) ab244948
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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 9, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 14" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 12.6" } ]
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Recovery

[ { "sample": "Milk", "range": "100 - 109 %", "average": "= 106" }, { "sample": "Serum", "range": "86 - 91 %", "average": "= 89" }, { "sample": "EDTA Plasma", "range": "76 - 82 %", "average": "= 79" }, { "sample": "Heparin Plasma", "range": "88 - 91 %", "average": "= 90" }, { "sample": "Citrate plasma", "range": "70 - 82 %", "average": "= 76" }, { "sample": "Cell culture media", "range": "108 - 118 %", "average": "= 114" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information Prolactin/PRL
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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