AB316252

Human Properdin/PFC ELISA Kit

SimpleStep
Recombinant
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Human Properdin/PFC ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Properdin/PFC with a sensitivity of 25.043 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

PFC, CFP, Properdin, Complement factor P

3 Images

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

sELISA

Supplier Data

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

Interpolated concentration of native Properdin/PFC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human urine 50% and stimulated PBMC cell culture supernatant (treated with 1.5% PHA-M for 46 hrs) 1 : 8. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

Interpolated concentration of native Properdin/PFC was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 1 : 8,000, plasma (citrate) 1 : 5,000, plasma (EDTA) 1 : 5,000, and plasma (heparin) 1 : 5,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Supplier Data

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

Example of human Properdin/PFC standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, EDTA Plasma, Urine, Cell culture supernatant, Citrate plasma, Heparin Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 25.043 pg/mL

Range

62.5 - 4000 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human Properdin/PFC SimpleStep ELISA^® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Properdin/PFC protein in Serum, EDTA Plasma, Cit Plasma, Hep Plasma, Urine, Cell culture supernatant. Quantitate Human Properdin/PFC with 25.043 pg/ml sensitivity.

SimpleStep ELISA^® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details. Our SimpleStep ELISA^® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA^® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA^® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.3" } ]

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Recovery

[ { "sample": "Serum", "range": "88 - 96 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "92 - 105 %", "average": "= 97" }, { "sample": "Citrate plasma", "range": "89 - 95 %", "average": "= 93" }, { "sample": "Heparin Plasma", "range": "95 - 105 %", "average": "= 101" }, { "sample": "Urine", "range": "88 - 94 %", "average": "= 91" }, { "sample": "Cell culture supernatant", "range": "96 - 106 %", "average": "= 101" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Properdin also known as factor P or complement factor P plays an essential role in the immune system. It is a serum protein with a molecular mass of approximately 53 kDa. Properdin is most commonly expressed in monocytes neutrophils and activated macrophages. It serves as a positive regulator in the alternative pathway of the complement system providing stability to the complement convertase complexes C3bBb and C3bBbC3b.

Biological function summary

Properdin enhances the activity of the complement system by stabilizing the C3 and C5 convertase enzyme complexes. These complexes are critical in the amplification of the complement cascade leading to pathogen opsonization and lysis. Properdin does not form part of a complex alone but helps existing complement complexes carry out their functions more effectively. By supporting these complexes properdin contributes to immune surveillance and the host's response to microbial invaders.

Pathways

Properdin operates within the alternative complement pathway and plays a pivotal role in immune defense. It interacts with other components such as C3 and C5 contributing to the pathway's ability to clear pathogens and damaged cells. Properdin's activity also indirectly influences other related proteins in the complement system supporting a coordinated immune response that involves factors beyond the alternative pathway itself.

Properdin deficiencies or dysregulations can lead to increased susceptibility to infections particularly by Neisseria species accounting for recurrent meningococcal disease. Additionally abnormal properdin function links to autoimmune conditions such as systemic lupus erythematosus. This connection is due to its relationship with various complement factors such as C3 which are vital for maintaining immune homeostasis.

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Product protocols

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Target data

A positive regulator of the alternate pathway (AP) of complement (PubMed : 20382442, PubMed : 28264884). It binds to and stabilizes the C3- and C5-convertase enzyme complexes (PubMed : 20382442, PubMed : 28264884). Inhibits CFI-CFH mediated degradation of Complement C3 beta chain (C3b) (PubMed : 31507604).

See full target information CFP

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human Properdin/PFC ELISA Kit

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

Sandwich ELISA - Human Properdin/PFC ELISA Kit (AB316252)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Proteins & Peptides

Proteins & Peptides

Proteins & Peptides

Product promise