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AB309441

Human Protective protein/Cathepsin A (PPCA) ELISA Kit

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Human Protective protein/Cathepsin A (PPCA) ELISA Kit is a Sandwich ELISA for the measurement of Human Protective protein/Cathepsin A (PPCA) in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

PPGB, CTSA, Lysosomal protective protein, Carboxypeptidase C, Carboxypeptidase L, Cathepsin A, Protective protein cathepsin A, Protective protein for beta-galactosidase, PPCA

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Sandwich ELISA - Human Protective protein/Cathepsin A (PPCA) ELISA Kit (AB309441)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protective protein/Cathepsin A (PPCA) ELISA Kit (AB309441)

Example of standard curve using ab309441. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.016 ng/mL

Range

0.016 - 10 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB250368

Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10434] - BSA and Azide free (AB250368)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Protective protein/Cathepsin A (PPCA) ELISA Kitt is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Protective protein/Cathepsin A (PPCA) in serum, plasma, and cell culture supernatants. This assay employs an antibody specific for Human Protective protein/Cathepsin A (PPCA) coated on a 96-well plate.
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Recovery

[ { "sample": "Plasma", "range": "72 - 86 %", "average": "= 78.22" }, { "sample": "Serum", "range": "72 - 125 %", "average": "= 88.27" }, { "sample": "Cell culture media", "range": "79 - 139 %", "average": "= 99.42" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309441-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB309441-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human Cathepsin A/CTSA Protein Standard", "number":"AB309441-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB309441-CMP12", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB309441-CMP06", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB309441-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Cathepsin A/CTSA Antibody-coated ELISA Plate", "number":"AB309441-CMP02", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB309441-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human Cathepsin A/CTSA Detection Antibody", "number":"AB309441-CMP09", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protective protein/Cathepsin A (PPCA) also known as cathepsin A or protective proteins is an enzyme with a molecular mass of approximately 54 kDa. The enzyme is expressed widely in different tissues including liver kidney and the central nervous system. Mechanically PPCA plays a role as a carboxypeptidase with serine-type protease activity important for the stabilization and activation of lysosomal enzymes such as beta-galactosidase and neuraminidase. This stabilization is vital for proper lysosomal function and the breakdown of glycoproteins within the cell.
Biological function summary

In conjunction with these enzymes PPCA forms part of the lysosomal multienzyme complex. It regulates the degradation of sialylated glycoconjugates by ensuring the structural integrity and enzymatic activity of lysosomal enzymes. This regulation prevents the accumulation of substrate materials which can disrupt cellular homeostasis. PPCA's interaction with beta-galactosidase and neuraminidase enables the proper catabolism of glycoproteins and glycolipids which is essential for cellular functions.

Pathways

PPCA functions within the lysosomal storage and sialic acid catabolism pathways. These pathways are significant for the degradation and recycling of macromolecules in the lysosome maintaining cellular health. In the sialic acid metabolism pathway PPCA pairs with neuraminidase to break down sialylated substrates while interacting closely with beta-galactosidase in glycoprotein catabolism. These interactions highlight its role in preserving the efficiency of lysosomal degradation processes.

Mutations in the PPCA gene can lead to diseases like galactosialidosis and neuraminidase deficiency. Galactosialidosis arises from defective cathepsin A activity disrupting normal glycoprotein breakdown and resulting in storage disorders. This condition involves both beta-galactosidase and neuraminidase which accumulate as a result of PPCA dysfunction. Similarly neuraminidase deficiency exhibits symptoms due to impaired degradation of sialic acids indicating the necessity of PPCA in disease prevention.
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Product protocols

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Target data

Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins.
See full target information CTSA
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websiteProtocolBooklet
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Product promise

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