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AB137988

Human Protein C ELISA Kit

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Human Protein C ELISA Kit is a Competitive ELISA for the measurement of Human Protein C in Human in Biofluids samples.

View Alternative Names

Vitamin K-dependent protein C, Anticoagulant protein C, Autoprothrombin IIA, Blood coagulation factor XIV, PROC

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Competitive ELISA - Human Protein C ELISA Kit (AB137988)
  • cELISA

Supplier Data

Competitive ELISA - Human Protein C ELISA Kit (AB137988)

Representative Standard Curve using ab137988

Competitive ELISA - Human Protein C ELISA Kit (AB137988)
  • cELISA

Supplier Data

Competitive ELISA - Human Protein C ELISA Kit (AB137988)

Representative Standard Curve using ab137988

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 76 ng/mL

Range

0.094 - 6 µg/mL

Assay time

3h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Protein C Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein C in plasma and serum.

A Protein C specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein C is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein C captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.7" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.1" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137988-1x96Tests", "assetComponentDetails": [ { "size":"1 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137988-CMP06", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137988-CMP09", "productcode":"" }, { "size":"1 x 1 Vial", "name":"1X Biotinylated Human Protein C (Lyophilized)", "number":"AB137988-CMP01", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137988-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Protein C Microplate (12 x 8 well strips)", "number":"AB137988-CMP03", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Protein C Standard", "number":"AB137988-CMP04", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137988-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Diluent M Concentrate", "number":"AB137988-CMP02", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137988-CMP07", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein C is a vitamin K-dependent serine protease enzyme that plays an important role in blood coagulation. It is also known as autoprothrombin IIA or blood coagulation factor XIV. The molecular weight of Protein C is approximately 62 kDa. It is synthesized in the liver and circulates in the plasma as an inactive zymogen. The protein becomes activated on the surface of endothelial cells and platelets where it plays an anticoagulant role.
Biological function summary

Protein C serves as an important regulator in the coagulation pathway. Upon activation it joins with a cofactor known as Protein S to form the activated Protein C complex which inactivates clotting factors Va and VIIIa. This inactivation leads to a reduction in thrombin formation thereby preventing excessive blood clotting. The activated C complex works efficiently on phospholipid surfaces such as those found on cellular membranes.

Pathways

Protein C activation is an important step in the anticoagulant pathway and works to balance the procoagulant actions of other factors. Protein C functions in tandem with proteins such as thrombin which transforms it into its active form when bound to thrombomodulin on the endothelial cell surface. The activated Protein C pathway ensures proper blood flow by preventing unchecked clot propagation and is intricately tied with pathways involving thrombin and factors Va and VIIIa.

Protein C deficiency is associated with an increased risk of developing thromboembolic disorders like deep vein thrombosis and pulmonary embolism. Deficiency can be congenital or acquired resulting in uncontrolled coagulation and increased susceptibility to clot formation. Additionally impaired activity of protein C is related to a disorder known as purpura fulminans which is characterized by blood clot formation and skin necrosis. The relation of Protein C to these conditions often involves dysfunctional interactions with factors Va VIIIa and thrombin.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein C is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PubMed : 25618265). Exerts a protective effect on the endothelial cell barrier function (PubMed : 25651845).
See full target information PROC
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Associated Products

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Alternative Product
Assay Kits

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Human Protein C ELISA Kit

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