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AB125969

Human Protein S ELISA Kit

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(2 Publications)

Human Protein S ELISA Kit is a Competitive ELISA for the measurement of Human Protein S in Human in Biofluids samples.

View Alternative Names

PROS, PROS1, Vitamin K-dependent protein S

1 Images
Competitive ELISA - Human Protein S ELISA Kit (AB125969)
  • cELISA

Supplier Data

Competitive ELISA - Human Protein S ELISA Kit (AB125969)

Representative Standard Curve using ab125969

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum

Reacts with

Human

Assay type

Competitive

Sensitivity

= 0.38 µg/mL

Range

0.5 - 8 µg/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "cELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam’s Protein S Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Human protein S in plasma and serum samples.

A Protein S specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Protein S is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Protein S captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "6.2" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.3" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein S also known as PROS1 is a vitamin K-dependent plasma glycoprotein with a molecular mass of approximately 75 kDa. It is synthesized mainly in the liver but also found in other tissues such as the endothelium megakaryocytes and osteoblasts. This protein plays a significant role in the regulation of blood coagulation by serving as a non-enzymatic cofactor for activated protein C (APC) in the proteolytic degradation of coagulation factors Va and VIIIa.
Biological function summary

The integrated function of Protein S within the coagulation cascade cannot be overemphasized. It acts synergistically with APC to limit thrombin generation therefore facilitating anticoagulation. Protein S is part of a complex with APC that exerts a potent anticoagulant effect. While free Protein S shows functional activity a large fraction circulates in complex with C4b-binding protein where it exhibits limited cofactor activity.

Pathways

The anticoagulant role of Protein S anchors it within the critical pathways like the protein C pathway impacting hemostasis. It directly associates with proteins such as Factor Va and Factor VIIIa and indirectly with proteins like thrombin. The regulation of coagulation through these pathways highlights its contribution to maintaining vascular integrity and preventing thrombosis.

Protein S deficiency has clinical implications in thrombotic conditions including deep vein thrombosis (DVT) and pulmonary embolism (PE). Such deficiencies can be hereditary or acquired often linked to disorders affecting vitamin K metabolism or liver function. Protein C another key anticoagulant closely interacts with Protein S in these disorders highlighting their collaborative effort in preventing excessive clot formation.
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Product protocols

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Target data

Anticoagulant plasma protein; it is a cofactor to activated protein C in the degradation of coagulation factors Va and VIIIa. It helps to prevent coagulation and stimulating fibrinolysis.
See full target information PROS1
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

AMB Express 14:66 PubMed38842656

2024

Microbial reduction of prebagged human plasma using 405 nm light and its effects on coagulation factors.

Applications

Unspecified application

Species

Unspecified reactive species

Caitlin F Stewart,Preston McGoldrick,John G Anderson,Scott J MacGregor,Chintamani D Atreya,Michelle Maclean

Oncotarget 9:14862-14881 PubMed29599912

2018

Coagulation cascade and complement system in systemic lupus erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liang,Shang-Bo Xie,Chang-Hao Wu,Yuan Hu,Qin Zhang,Si Li,Yin-Guang Fan,Rui-Xue Leng,Hai-Feng Pan,Hua-Bao Xiong,Dong-Qing Ye
View all publications
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