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AB137989

Human Protein Z ELISA Kit

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(1 Publication)

Human Protein Z ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Protein Z in Human in Biofluids samples.

View Alternative Names

Vitamin K-dependent protein Z, PROZ

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ELISA - Human Protein Z ELISA Kit (AB137989)
  • ELISA

Lab

ELISA - Human Protein Z ELISA Kit (AB137989)

Protein Z measured in biological fluids (duplicates +/- SD). Human plasma and serum were tested at a dilution factor range of 50-1350, other fluids 1-10.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Serum, Milk, Cerebral Spinal Fluid, Urine

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

> 64 pg/mL

Range

0.098 - 25 ng/mL

Assay time

4h

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's Protein Z Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Protein Z in plasma, serum, urine, milk, and cell culture supernatants.

A Protein Z specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently a Protein Z specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Conjugate is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Protein Z captured in plate.

The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.8" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab137989-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Vial", "name":"Protein Z Standard", "number":"AB137989-CMP03", "productcode":"" }, { "size":"1 x 100 µL", "name":"60X Biotinylated Human Protein Z Antibody", "number":"AB137989-CMP10", "productcode":"" }, { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB137989-CMP06", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB137989-CMP05", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB137989-CMP09", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB137989-CMP07", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB137989-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Protein Z Microplate (12 x 8 well strips)", "number":"AB137989-CMP04", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB137989-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Protein Z also known as PZ is a vitamin K-dependent plasma protein with a molecular mass of approximately 62 kDa. It is primarily expressed in the liver and circulates in the blood. This protein plays a mechanical role in the coagulation system by interacting with the protein Z-dependent protease inhibitor which downregulates blood coagulation contributing to hemostasis. Protein Z is different from other coagulation proteins due to its lack of enzymatic activity but impacts the regulation of thrombin formation.
Biological function summary

Protein Z serves as a significant factor in the coagulation cascade being a part of the protein C pathway complex. Although it lacks enzymatic action Protein Z interacts with other proteins such as Factor Xa and Protein Z-dependent protease inhibitor to exert its effects. Its involvement helps modulate clot formation and stabilize the coagulation process impacting blood flow regulation.

Pathways

Protein Z is intricately linked to the coagulation and thrombosis pathways. It associates with proteins including Factor Xa and Protein C. Protein Z assists in regulating thrombin generation by inhibiting excess coagulation maintaining a balance within the hemostatic system. This interaction ensures the rapid and effective stopping of blood flow when required while preventing unwarranted thrombosis a critical aspect of circulatory system maintenance.

Protein Z levels link to thrombosis and hemorrhagic disorders. Abnormalities in Protein Z levels have connections with deep vein thrombosis due to its role in regulating coagulation balance. The protein Z-dependent protease inhibitor's association highlights its significance in clot formation and thrombosis prevention. Additionally deficiencies in Protein Z have connections with bleeding disorders emphasizing the protein's role in maintaining a balanced coagulation process essential for preventing excessive bleeding or clotting.
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Product protocols

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Target data

Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids.
See full target information PROZ
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Ginekologia polska 93:987-992 PubMed35072242

2022

Expression and diagnostic value of plasma protein Z and protein Z-dependent protease inhibitor in fetal growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Ci Pan,Yulian Huang,Xiaopei Guo,Cunhua Gu,Jing Guo,Xiaohua Luo
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