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AB322365

Human Protein Z ELISA Kit

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Human Protein Z ELISA Kit is a single-wash 90-min Simplestep used to quantify Human Protein Z with a sensitivity of 7 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

Vitamin K-dependent protein Z, PROZ

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Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations. Undiluted samples are as follows : Hep G2 cell culture supernatant 25% and milk 1 : 25. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Protein Z concentration was determined to be 5,437 ng/mL with a range of 3,862 – 10,048 ng/mL.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations. Undiluted samples are as follows : serum 1 : 5,000, plasma (citrate) 1 : 2,500, plasma (EDTA) 1 : 2,500, and plasma (heparin) 1 : 5,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1 : 5,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Protein Z concentration was determined to be 3,666 ng/mL with a range of 1,195 – 7,833 ng/mL.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations. Undiluted samples are as follows : urine 25% and saliva 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Example of human Protein Z standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)
  • sELISA

Supplier Data

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365)

Example of human Protein Z standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 7 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Protein Z SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Protein Z protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Saliva, Milk. Quantitate Human Protein Z with 7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 7" }, { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 3.1" } ]
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Recovery

[ { "sample": "Serum", "range": "97 - 100 %", "average": "= 98" }, { "sample": "Citrate plasma", "range": "89 - 98 %", "average": "= 93" }, { "sample": "EDTA Plasma", "range": "95 - 110 %", "average": "= 102" }, { "sample": "Heparin Plasma", "range": "97 - 100 %", "average": "= 98" }, { "sample": "Cell culture supernatant", "range": "98 - 99 %", "average": "= 98" }, { "sample": "Urine", "range": "100 - 110 %", "average": "= 105" }, { "sample": "Saliva", "range": "78 - 79 %", "average": "= 79" }, { "sample": "Milk", "range": "92 - 107 %", "average": "= 98" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids.
See full target information PROZ
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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