Human Protein Z ELISA Kit (ab322365)

Human Protein Z ELISA Kit is a single-wash 90-min SimpleStep ELISA® for the quantitative measurement of Human Protein Z in Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine samples.

Be the first to review this product! Submit a review

Images

Sandwich ELISA - Human Protein Z ELISA Kit (AB322365), expandable thumbnail

Key facts

Detection method: Colorimetric
Sample types: Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 25 - 1600 pg/mL
Assay time: 1h 30m
Sensitivity: = 7 pg/mL

Reactivity data

Application	Reactivity	Dilution info	Notes
Application sELISA	Reactivity Reacts	Dilution info -	Notes -

Target data

See full target information PROZ

Function

Appears to assist hemostasis by binding thrombin and promoting its association with phospholipid vesicles. Inhibits activity of the coagulation protease factor Xa in the presence of SERPINA10, calcium and phospholipids.

Alternative names

Vitamin K-dependent protein Z, PROZ

What's included?

10 x 96 Tests

Components

10X Human Protein Z Detector Antibody

10 x 600 µL

10X Wash Buffer PT (ab206977)

2 x 200 mL

Antibody Diluent CPR2

10 x 6 mL

Human Protein Z Lyophilized Recombinant Protein

20 x 1 Vial

Lyophilized Human Protein Z Capture Antibody

10 x 1 Vial

Plate Seal

10 x 1 Unit

Sample Diluent NS (ab193972)

1 x 12 mL

SimpleStep Pre-Coated 96-Well Microplate (ab206978)

10 x 1 Unit

Stop Solution

1 x 120 mL

TMB Development Solution

1 x 120 mL

Key facts

Detection method: Colorimetric
Sample types: Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant, Urine
Assay type: Sandwich (quantitative)
Reactive species: Human
Range: 25 - 1600 pg/mL
Assay time: 1h 30m
Assay Platform: Microplate (12 x 8 well strips)
Sensitivity: = 7 pg/mL
General recovery: 98%

Precision

Intra assay

Sample	n	mean	SD	C.V.
Sample Serum	n 8	mean -	SD -	C.V. = 7

Inter assay

Sample	n	mean	SD	C.V.
Sample Serum	n 3	mean -	SD -	C.V. = 3.1

Recovery

Sample specific recovery

Sample type	Average %	Range
Sample type Serum	Average % = 98	Range 97 - 100 %
Sample type Citrate plasma	Average % = 93	Range 89 - 98 %
Sample type EDTA Plasma	Average % = 102	Range 95 - 110 %
Sample type Heparin Plasma	Average % = 98	Range 97 - 100 %
Sample type Cell culture supernatant	Average % = 98	Range 98 - 99 %
Sample type Urine	Average % = 105	Range 100 - 110 %
Sample type Saliva	Average % = 79	Range 78 - 79 %
Sample type Milk	Average % = 98	Range 92 - 107 %

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: +4°C

Notes

Human Protein Z SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Protein Z protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant, Urine, Saliva, Milk. Quantitate Human Protein Z with 7 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less

-High sensitivity, specificity and reproducibility from superior antibodies

-Fully validated in biological samples

-96-wells plate breakable into 12 x 8 wells strips, available in 10-pack (10 x 96-well plates)

-Also available in a fully validated 384-well format.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Example of human Protein Z standard curve in 1X Wash Buffer PT. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 1:5,000 human serum. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Example of human Protein Z standard curve in 1X Wash Buffer PT in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Serum of ten individual healthy human male donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Protein Z concentration was determined to be 5,437 ng/mL with a range of 3,862 – 10,048 ng/mL.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Serum of ten individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). The mean Protein Z concentration was determined to be 3,666 ng/mL with a range of 1,195 – 7,833 ng/mL.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations. Undiluted samples are as follows: urine 25% and saliva 50%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations. Undiluted samples are as follows: Hep G2 cell culture supernatant 25% and milk 1:25. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.
Sandwich ELISA - Human Protein Z ELISA Kit (ab322365)
Interpolated concentration of native Protein Z was measured in duplicate at different sample concentrations. Undiluted samples are as follows: serum 1:5,000, plasma (citrate) 1:2,500, plasma (EDTA) 1:2,500, and plasma (heparin) 1:5,000. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Wash Buffer PT.