AB264615

Human PSA ELISA Kit

Recombinant
SimpleStep
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Human PSA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PSA with a sensitivity of 8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin

9 Images

ELISA

Supplier Data

ELISA - Human PSA ELISA Kit (AB264615)

sELISA

Lab

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Interpolated concentration of native PSA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% human plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

sELISA

Lab

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Example of human PSA standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Human PSA standard curve comparison.

Standard Curve comparison between human PSA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

sELISA

Supplier Data

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Example of human PSA standard curve in Sample Diluent NS.

The PSA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Supplier Data

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Interpolated concentrations of native PSA in human serum and plasma (citrate) samples.

The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 100% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 630 pg/mL in serum, 1,300 pg/mL in plasma (citrate).

sELISA

Supplier Data

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Urine from both pooled male and female donors was measured in duplicate at 1 : 200 dilution.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Female urine was below the lower limit of detection and 174 ng/mL in male urine.

sELISA

Supplier Data

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Serum from eight individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 826 pg/mL with a range of 234 – 2,201 pg/mL.

sELISA

Supplier Data

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Interpolated concentrations of native PSA in human male urine samples.

The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows : male urine 1 : 200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 174 ng/mL in male urine.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8 pg/mL

Range

39 - 2500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human PSA ELISA Kit ab264615 is a rapid single-wash 90-min sandwich ELISA kit to measure Human PSA in Urine, Plasma (Citrate, EDTA, Heparin), Cell culture supernatant, Serum. This Simplestep sensitivity is 8 pg/mL.

How the assay works

Human PSA SimpleStep ELISA^®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA^® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA^® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA^® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.

This immunoassay is calibrated against a highly purified human PSA. The NIBSC/WHO unclassified purified human PSA preparation 96/668 was evaluated in this kit.
The dose response curve of the unclassified standard PSA parallels the SimpleStep standard curve. To convert sample values obtained with the SimpleStep Human PSA kit to approximate NIBSC units, use the equation below.

NIBSC (96/668) approximate value (pg/mL) = 0.4 x SimpleStep Human PSA value (pg/mL).

Native purified PSA-ACT were prepared at 2,500 pg/mL and assayed for cross reactivity. Cross reactivity was observed. The antibodies used in this assay recognize total PSA levels.

Human PSA ELISA Kit ab264615 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

How other researchers are using Human PSA ELISA Kit ab264615

Human PSA is a glycoprotein produced by the prostate gland and is often monitored in serum as a biomarker for prostate cancer.

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

-Anti-Prostate Specific Antigen antibody [EPR22271-97] - BSA and Azide free (Capture) ab242191
- Anti-Prostate Specific Antigen antibody [EPR22271-81] - BSA and Azide free (Detector) ab242192

Related and Recommended products

Human PSA is a glycoprotein produced by the prostate gland and is often monitored in serum as a biomarker for prostate cancer.

Other ELISA kits to measure related family members and binding partners include:

- Human Serpin A5 ELISA Kit ab309174
- Human Tissue Kallikrein (KLK1) ELISA Kit- Extracellular ab320795
- Human Kallikrein 6/KLK6 ELISA Kit ab287163
- Human Kallikrein 7/KLK7 ELISA Kit ab287175
- Human Kallikrein 10 ELISA Kit ab279406

Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland, but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels. This assay detects both total and free PSA levels.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Urine", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.4" }, { "reproducibilityType": "Intra", "sample": "Urine", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.9" } ]

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Recovery

[ { "sample": "Cell culture supernatant", "range": "86 - 97 %", "average": "= 92" }, { "sample": "Serum", "range": "95 - 103 %", "average": "= 99" }, { "sample": "EDTA Plasma", "range": "80 - 94 %", "average": "= 88" }, { "sample": "Urine", "range": "112 - 120 %", "average": "= 116" }, { "sample": "Heparin Plasma", "range": "85 - 88 %", "average": "= 86" }, { "sample": "Citrate plasma", "range": "108 - 120 %", "average": "= 115" } ]

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What's included?

{ "values": { "1x384Tests": { "sellingSize": "1 x 384 Tests", "publicAssetCode":"ab264615-1x384Tests", "assetComponentDetails": [ { "size":"1 x 600 µL", "name":"10X Human PSA Capture Antibody", "number":"AB264615-CMP07" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264615-CMP10" }, { "size":"1 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264615-CMP02" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264615-CMP06" }, { "size":"2 x 1 Vial", "name":"Human PSA Lyophilized Recombinant Protein", "number":"AB264615-CMP01" }, { "size":"1 x 600 µL", "name":"10X Human PSA Detector Antibody", "number":"AB264615-CMP08" }, { "size":"2 x 12 mL", "name":"TMB Development Solution", "number":"AB264615-CMP05" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264615-CMP09" }, { "size":"2 x 12 mL", "name":"Stop Solution", "number":"AB264615-CMP04" }, { "size":"1 x 1 Unit", "name":"384 well CaptSure™ microplates", "number":"AB264615-CMP11" } ] }, "10x96Tests": { "sellingSize": "10 x 96 Tests", "publicAssetCode":"ab264615-10x96Tests", "assetComponentDetails": [ { "size":"1 x 120 mL", "name":"Stop Solution", "number":"AB264615-CMP04" }, { "size":"1 x 200 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264615-CMP10" }, { "size":"1 x 120 mL", "name":"TMB Development Solution", "number":"AB264615-CMP05" }, { "size":"10 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264615-CMP06" }, { "size":"1 x 6000 µL", "name":"10X Human PSA Capture Antibody", "number":"AB264615-CMP07" }, { "size":"2 x 250 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264615-CMP02" }, { "size":"2 x 10 Vial", "name":"Human PSA Lyophilized Recombinant Protein", "number":"AB264615-CMP01" }, { "size":"1 x 6000 µL", "name":"10X Human PSA Detector Antibody", "number":"AB264615-CMP08" }, { "size":"10 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264615-CMP03" }, { "size":"10 x 1 Unit", "name":"Plate Seals", "number":"AB264615-CMP09" } ] }, "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab264615-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB264615-CMP04", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB264615-CMP06", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSA Capture Antibody", "number":"AB264615-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PSA Lyophilized Recombinant Protein", "number":"AB264615-CMP01", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB264615-CMP10", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB264615-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB264615-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB264615-CMP03", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB264615-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSA Detector Antibody", "number":"AB264615-CMP08", "productcode":"" } ] } } }

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.

Biological function summary

PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.

Pathways

PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.

PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.

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Product protocols

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Target data

Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

See full target information KLK3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

ACS nano 18:30848-30862 PubMed39463189

2024

Transdermal Minimally Invasive Optical Multiplex Detection of Protein Biomarkers by Nanopillars Array-Embedded Microneedles.

Applications

Unspecified application

Species

Unspecified reactive species

Adva Raz,Hila Gubi,Adam Cohen,Fernando Patolsky

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Human PSA ELISA Kit

ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Sandwich ELISA - Human PSA ELISA Kit (AB264615)

Key facts

Detection method

Sample types

Reacts with

Assay type

Sensitivity

Range

Assay time

Assay Platform

Reactivity data

Product details

Precision

Recovery

What's included?

Properties and storage information

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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