Human PSA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PSA with a sensitivity of 8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39 - 2500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 8 pg/mL
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.
Alternative names
APS, KLK3, Prostate-specific antigen, PSA, Gamma-seminoprotein, Kallikrein-3, P-30 antigen, Semenogelase, Seminin
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Human PSA ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PSA with a sensitivity of 8 pg/mL. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 39 - 2500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 8 | mean - | SD - | C.V. 5.9 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Urine | n 3 | mean - | SD - | C.V. 8.4 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 92 | Range 86 - 97 % |
Sample type Serum | Average % = 99 | Range 95 - 103 % |
Sample type EDTA Plasma | Average % = 88 | Range 80 - 94 % |
Sample type Urine | Average % = 116 | Range 112 - 120 % |
Sample type Heparin Plasma | Average % = 86 | Range 85 - 88 % |
Sample type Citrate plasma | Average % = 115 | Range 108 - 120 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PSA ELISA Kit (ab264615) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSA protein in edta plasma, hep plasma, serum, fetal bovine serum, urine, and cit plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSA with 8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Prostate specific antigen (PSA) or also commonly termed tissue Kallikrein-3 (KLK3) is a 34 kD serine protease from the Kallikrein family. PSA has very high expression in the prostate gland, but expression also has been reported in the breast and saliva. PSA functions by hydrolyzing semenogelin-1 leading to the liquefaction of the seminal coagulum. This is essential for the proper release of sperm during ejaculation. The majority (>70%) of PSA is known to form a complex with alpha-1-antichymotrypsin (PSA-ACT) in the blood. The remaining PSA exists as an unbound inactive enzyme known as Free PSA. PSA is best characterized as a clinical biomarker for Prostate Cancer. During prostate cancer progression, excess amounts of PSA are leaked into the blood dramatically elevating both Total and Free PSA levels. This assay detects both total and free PSA levels.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Prostate Specific Antigen (PSA) also known as kallikrein-related peptidase 3 (KLK3) is a serine protease with a mass of approximately 28.4 kDa. PSA is primarily expressed in the epithelial cells of the prostate gland. This glycoprotein is an important component in the seminal fluid where it performs its proteolytic activity that liquefies semen facilitating sperm motility.
- Biological function summary
PSA functions by cleaving the gel-forming proteins present in seminal coagulum. It does not operate as part of a larger protein complex. PSA also interacts with substrates like fibronectin and semenogelin which play roles in seminal coagulation and liquefaction. The expression of PSA in prostate tissue has made it a valuable biomarker for prostate function and pathological conditions.
- Pathways
PSA is involved in the kallikrein-kinin system which influences inflammation and blood pressure regulation. PSA is not directly a part of key metabolic or signaling pathways but it is co-expressed with key proteins like androgen receptor (AR) which modulates PSA's expression. The interplay between androgens and PSA highlights its role in hormone regulation within the male reproductive system.
- Associated diseases and disorders
PSA levels serve as an indicator for prostate cancer and benign prostatic hyperplasia (BPH). Elevated PSA levels often correlate with prostate cancer although other prostate conditions such as prostatitis can also increase PSA without cancer. Proteins like PCA3 and the AR are also connected in the context of prostate cancer providing a broader understanding of PSA's role in these diseases.
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8 product images
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Human PSA standard curve comparison.
Standard Curve comparison between human PSA SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Example of human PSA standard curve in Sample Diluent NS.
The PSA standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Interpolated concentrations of native PSA in human serum and plasma (citrate) samples.
The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100% and plasma (citrate) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 630 pg/mL in serum, 1,300 pg/mL in plasma (citrate).
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Interpolated concentrations of native PSA in human male urine samples.
The concentrations of PSA were measured in duplicates, interpolated from the PSA standard curves and corrected for sample dilution. Undiluted samples are as follows: male urine 1:200. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 174 ng/mL in male urine.
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Serum from eight individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSA concentration was determined to be 826 pg/mL with a range of 234 – 2,201 pg/mL.
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Urine from both pooled male and female donors was measured in duplicate at 1:200 dilution.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Female urine was below the lower limit of detection and 174 ng/mL in male urine.
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Example of human PSA standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PSA ELISA Kit (ab264615)
Interpolated concentration of native PSA was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 100% human plasma (citrate). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.
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