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AB315064

Human PSAP ELISA Kit

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Human PSAP ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PSAP with a sensitivity of 69.56 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

GLBA, SAP1, PSAP, Prosaposin, Proactivator polypeptide

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Sandwich ELISA - Human PSAP ELISA Kit (AB315064)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)

Interpolated concentration of native PSAP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human serum 10%, plasma (citrate) 10%, plasma (EDTA) 20%, and plasma (heparin) 10%. The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)

Interpolated concentration of native PSAP was measured in duplicate at different sample concentrations. Undiluted samples are as follows : A431 cell culture supernatant 1 : 125.  The interpolated dilution factor corrected values (to neat sample) are plotted (mean +/- SD, n=2). Sample dilutions are made in Sample Diluent NS.

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)

Serum of eight individual healthy human female donors was measured in duplicate. Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSAP concentration was determined to be 87 ng/mL with a range of 34 – 114 ng/mL.

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSAP ELISA Kit (AB315064)

Example of human PSAP standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Serum, Citrate plasma, EDTA Plasma, Heparin Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 69.56 pg/mL

Range

178.125 - 11400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PSAP SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSAP protein in Serum, Cit Plasma, EDTA Plasma, Hep Plasma, Cell culture supernatant. Quantitate Human PSAP with 69.56 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Human Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "7.2" }, { "reproducibilityType": "Inter", "sample": "Human Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.32" } ]
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Recovery

[ { "sample": "Serum", "range": "91 - 100 %", "average": "= 96" }, { "sample": "Citrate plasma", "range": "100 - 105 %", "average": "= 103" }, { "sample": "EDTA Plasma", "range": "100 - 112 %", "average": "= 106" }, { "sample": "Heparin Plasma", "range": "95 - 100 %", "average": "= 98" }, { "sample": "Cell culture supernatant", "range": "99 - 110 %", "average": "= 106" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.. Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 3.1.6.8), GM1 gangliosides by beta-galactosidase (EC 3.2.1.23) and globotriaosylceramide by alpha-galactosidase A (EC 3.2.1.22). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.. Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 3.1.4.12).. Prosaposin. Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.. Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases.
See full target information PSAP
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websiteProtocolBooklet
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