Human PSD95 ELISA Kit-Extracellular is a single-wash 90-min Simplestep used to quantify Human PSD95-Extracellular with a sensitivity of 8.806 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.875 - 1400 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 8.806 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (PubMed:26334723).
Alternative names
PSD95, DLG4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90
What's included?
Recommended products
Human PSD95 ELISA Kit-Extracellular is a single-wash 90-min Simplestep used to quantify Human PSD95-Extracellular with a sensitivity of 8.806 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)
Key facts
- Detection method
- Colorimetric
- Sample types
- Cerebral Spinal Fluid
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 21.875 - 1400 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 8.806 pg/mL
- General recovery
- 113%
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cerebral Spinal Fluid | n 8 | mean - | SD - | C.V. 11.3 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Cerebral Spinal Fluid | n 3 | mean - | SD - | C.V. 4.9 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cerebral Spinal Fluid | Average % = 113 | Range 108 - 118 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PSD95 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSD95 protein in Cerebral Spinal Fluid. Quantitate Human PSD95 with 8.806 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sandwich ELISA - Human PSD95 ELISA Kit-Extracellular (ab315072)
Example of human PSD95 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PSD95 ELISA Kit-Extracellular (ab315072)
Recombinant PSD95 was spiked into human cerebrospinal fluid and diluted in a 2-fold dilution series in Sample Diluent NS. The undiluted sample is 50% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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