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AB315072

Human PSD95 ELISA Kit-Extracellular

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Human PSD95 ELISA Kit-Extracellular is a single-wash 90-min Simplestep used to quantify Human PSD95-Extracellular with a sensitivity of 8.806 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Validated on a number of sample types including cerebrospinal fluid (CSF)

View Alternative Names

PSD95, DLG4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90

2 Images
Sandwich ELISA - Human PSD95 ELISA Kit-Extracellular (AB315072)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSD95 ELISA Kit-Extracellular (AB315072)

Example of human PSD95 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PSD95 ELISA Kit-Extracellular (AB315072)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSD95 ELISA Kit-Extracellular (AB315072)

Recombinant PSD95 was spiked into human cerebrospinal fluid and diluted in a 2-fold dilution series in Sample Diluent NS. The undiluted sample is 50% cerebrospinal fluid. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 8.806 pg/mL

Range

21.875 - 1400 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PSD95 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSD95 protein in Cerebral Spinal Fluid. Quantitate Human PSD95 with 8.806 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Cerebral Spinal Fluid", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "11.3" }, { "reproducibilityType": "Inter", "sample": "Cerebral Spinal Fluid", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "4.9" } ]
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Recovery

[ { "sample": "Cerebral Spinal Fluid", "range": "108 - 118 %", "average": "= 113" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315072-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315072-CMP08", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315072-CMP05", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS", "number":"AB315072-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Substrate", "number":"AB315072-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSD95 Capture Antibody", "number":"AB315072-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PSD95 Lyophilized Recombinant Protein", "number":"AB315072-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB315072-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSD95 Detector Antibody", "number":"AB315072-CMP02", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-Coated 96-Well Microplate", "number":"AB315072-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315072-CMP10", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (PubMed : 26334723).
See full target information DLG4
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websiteProtocolBooklet
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Product promise

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