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AB315071

Human PSD95 ELISA Kit-Intracellular

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Human PSD95 ELISA Kit-Intracellular is a single-wash 90-min Simplestep used to quantify Human PSD95-Intracellular with a sensitivity of 9.021 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader

View Alternative Names

PSD95, DLG4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90

3 Images
Sandwich ELISA - Human PSD95 ELISA Kit-Intracellular (AB315071)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSD95 ELISA Kit-Intracellular (AB315071)

Interpolated concentrations of human PSD95 in human brain cortex tissue extract, U-87 MG cell extract, and SH-SY5Y cell extract

Interpolated concentration of native PSD95 was measured in duplicate at different sample concentrations. Undiluted samples are as follows : human brain cortex extract 10 µg/mL, U-87 MG cell extract 800 µg/mL, and SH-SY5Y cell extract 500 µg/mL.  The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human PSD95 ELISA Kit-Intracellular (AB315071)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSD95 ELISA Kit-Intracellular (AB315071)

Example of human PSD95 standard curve in 1X Cell Extraction Buffer PTR. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PSD95 ELISA Kit-Intracellular (AB315071)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSD95 ELISA Kit-Intracellular (AB315071)

Interpolated concentrations of PSD95 in mouse and rat brain tissue extracts

Interpolated concentrations of native PSD95 were measured in duplicate at different sample concentrations. Undiluted samples are as follows : mouse brain extract 10 µg/mL and rat brain extract 10 µg/mL. The interpolated (from human standard curve) dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate, Tissue Extracts

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 9.021 pg/mL

Range

26.563 - 1700 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB32942

Anti-SV2A antibody

Western blot - Anti-SV2A antibody (AB32942)

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  • 8
Primary Antibodies

AB18258

Anti-PSD95 antibody - Synaptic Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-PSD95 antibody - Synaptic Marker (AB18258)

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Proteins & Peptides

AB131874

Recombinant Human PSD95 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human PSD95 protein (GST tag N-Terminus) (AB131874)

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  • 0
Proteins & Peptides

AB18661

Mouse PSD95 peptide

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PSD95 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSD95 protein in Cell Lysate, Tissue extracts. Quantitate Human PSD95 with 9.021 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.1" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "3.7" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "96 - 129 %", "average": "= 115" }, { "sample": "Tissue Extracts", "range": "67 - 100 %", "average": "= 89" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab315071-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB315071-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB315071-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB315071-CMP10", "productcode":"" }, { "size":"1 x 1 mL", "name":"50X Cell Extraction Enhancer Solution (ab193971)", "number":"AB315071-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB315071-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS", "number":"AB315071-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PSD95 Lyophilized Recombinant Protein", "number":"AB315071-CMP03", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB315071-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSD95 Capture Antibody", "number":"AB315071-CMP01", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSD95 Detector Antibody", "number":"AB315071-CMP02", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB315071-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seal", "number":"AB315071-CMP12", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins. Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (By similarity). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (PubMed : 26334723).
See full target information DLG4
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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