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AB243676

Human PSG1 ELISA Kit

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Human PSG1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PSG1 with a sensitivity of 11.5 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD66f, B1G1, PSBG1, PSGGA, PSG1, Pregnancy-specific beta-1-glycoprotein 1, PS-beta-G-1, PSBG-1, Pregnancy-specific glycoprotein 1, CD66 antigen-like family member F, Fetal liver non-specific cross-reactive antigen 1/2, PSG95, Pregnancy-specific beta-1 glycoprotein C/D, FL-NCA-1/2, PS-beta-C/D

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Sandwich ELISA - Human PSG1 ELISA Kit (AB243676)
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Supplier Data

Sandwich ELISA - Human PSG1 ELISA Kit (AB243676)

Interpolated concentrations of native PSG1 in pregnant human serum and SW480 cell culture supernatant samples.

The concentrations of PSG1 were measured in duplicates, interpolated from the PSG1 standard curves and corrected for sample dilution. Undiluted samples are as follows : pregnant human serum 1 : 20,000 and SW480 cell culture supernatant 30%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PSG1 concentration was determined to be 18.13 μg/mL in neat pregnant human serum and 0.0049 μg/mL in neat SW480 cell culture supernatant.

Sandwich ELISA - Human PSG1 ELISA Kit (AB243676)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSG1 ELISA Kit (AB243676)

Human PSG1 Standard Curve Comparison.

Standard Curve comparison between human PSG1 SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human PSG1 ELISA Kit (AB243676)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSG1 ELISA Kit (AB243676)

Example of human PSG1 standard curve in Sample Diluent NS.

The PSG1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Urine, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 11.5 pg/mL

Range

25 - 1600 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PSG1 ELISA Kit (ab243676) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PSG1 protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSG1 with 11.5 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "1.4" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.6" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "88 - 106 %", "average": "= 97" }, { "sample": "Serum", "range": "94 - 115 %", "average": "= 101" }, { "sample": "EDTA Plasma", "range": "82 - 94 %", "average": "= 88" }, { "sample": "Urine", "range": "84 - 90 %", "average": "= 88" }, { "sample": "Heparin Plasma", "range": "76 - 94 %", "average": "= 88" }, { "sample": "Citrate plasma", "range": "77 - 94 %", "average": "= 88" }, { "sample": "Cell culture media", "range": "89 - 98 %", "average": "= 93" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab243676-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB243676-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB243676-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB243676-CMP07", "productcode":"" }, { "size":"2 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB243676-CMP05", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB243676-CMP08", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB243676-CMP09", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 5BI", "number":"AB243676-CMP01", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PSG1 Lyophilized Recombinant Protein", "number":"AB243676-CMP04", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSG1 Capture Antibody", "number":"AB243676-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSG1 Detector Antibody", "number":"AB243676-CMP03", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

See full target information PSG1
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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