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AB289697

Human PSGL-1 ELISA Kit

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Human PSGL-1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PSGL-1 with a sensitivity of 0.742 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

CD162, P-selectin glycoprotein ligand 1, PSGL-1, Selectin P ligand, SELPLG

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Sandwich ELISA - Human PSGL-1 ELISA Kit (AB289697)
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Supplier Data

Sandwich ELISA - Human PSGL-1 ELISA Kit (AB289697)

Example of Human PSGL-1 standard curve in 1X Cell Extraction Buffer PTR.

Example of Human PSGL-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Sandwich ELISA - Human PSGL-1 ELISA Kit (AB289697)
  • sELISA

Supplier Data

Sandwich ELISA - Human PSGL-1 ELISA Kit (AB289697)

Example of Human PSGL-1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Interpolated concentration of native PSGL-1 was measured in duplicate at different sample concentrations. Undiluted samples are 8 μg/mL Jurkat cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.742 pg/mL

Range

3.125 - 200 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PSGL-1 ELISA Kit (ab289697) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Human PSGL-1 in cell extract samples. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PSGL-1 with 0.742 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "9.4" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "99 - 110 %", "average": "= 103" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab289697-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB289697-CMP04", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB289697-CMP11", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB289697-CMP12", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB289697-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB289697-CMP10", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSGL-1 Capture Antibody", "number":"AB289697-CMP08", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BI", "number":"AB289697-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human PSGL-1 Lyophilized Recombinant Protein", "number":"AB289697-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human PSGL-1 Detector Antibody", "number":"AB289697-CMP09", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB289697-CMP03", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB289697-CMP06", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.. (Microbial infection) Acts as a receptor for enterovirus 71.
See full target information SELPLG
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websiteProtocolBooklet
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Product promise

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For full details, please see our Terms & Conditions

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