Human PTH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PTH with a sensitivity of 0.761 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
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- Medicine 101:e320092022Production of parathyroid-like cells from thyroid stem cells in co-culture environment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesÖzge Karabiyik Acar,Gülçin Delal Nozhatzadeh,Alperen Tuncer,Gamze Torun Köse,Ezgi Hacihasanoğlu,Fikrettin Sahin,Erhan AysanPubMed 36482540
- Nature communications 11:62492020Cytosolic sequestration of the vitamin D receptor as a therapeutic option for vitamin D-induced hypercalcemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniela Rovito et. al.PubMed 33288743
- eLife 9:2020High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOk-Hee Kim,Carmen J Booth,Han Seok Choi,Jinwook Lee,Jinku Kang,June Hur,Woo Jin Jung,Yun-Shin Jung,Hyung Jin Choi,Hyeonjin Kim,Joong-Hyuck Auh,Jung-Wan Kim,Ji-Young Cha,Young Jae Lee,Cheol Soon Lee,Cheolsoo Choi,Yun Jae Jung,Jun-Young Yang,Seung-Soon Im,Dae Ho Lee,Sun Wook Cho,Young-Bum Kim,Kyong Soo Park,Young Joo Park,Byung-Chul OhPubMed 32271147
- PloS one 14:e02191092019Circulating levels of free 25(OH)D increase at the onset of rheumatoid arthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVidyanand Anaparti,Xiaobo Meng,Mahadevappa Hemshekhar,Irene Smolik,Neeloffer Mookherjee,Hani El-GabalawyPubMed 31557191
- Molecular medicine reports 18:261-2672018A novel PNPLA6 compound heterozygous mutation identified in a Chinese patient with Boucher‑Neuhäuser syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRuizhi Zheng et. al.PubMed 29749493
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.69 - 300 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.761 pg/mL
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Alternative names
Parathyroid hormone, PTH, Parathormone, Parathyrin
What's included?
Recommended products
Human PTH ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PTH with a sensitivity of 0.761 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Different formats for different needs: 10x96 plates for bulk orders and 384-well plate for higher throughput
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 5 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Heparin Plasma, Citrate plasma, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 4.69 - 300 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 0.761 pg/mL
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 8 | C.V. 1.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Serum | n 3 | C.V. 3.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Serum | Average % = 88 | Range 88 - 89 % |
Sample type EDTA Plasma | Average % = 90 | Range 88 - 92 % |
Sample type Heparin Plasma | Average % = 83 | Range 81 - 84 % |
Sample type Citrate plasma | Average % = 82 | Range 81 - 82 % |
Sample type Cell culture media | Average % = 105 | Range 104 - 106 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PTH ELISA Kit ab230931 is a rapid single-wash 90-min Sandwich ELISA to measure Human PTH in citrate plasma, EDTA plasma, heparin plasma, serum. This SimpleStep sensitivity is 0.761 pg/mL.
How the assay works
Human PTH SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.
Assay Specificity
Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.
Human PTH ELISA Kit ab230931 protocol summary
1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm
Save your precious samples
Our 384-well Human PTH ELISA Kit only requires a maximum of 12.5µL sample
Design your own immunoassay
We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:
- Anti-Parathyroid Hormone antibody [EPR9939-66] - BSA and Azide free (Capture) Anti-Parathyroid Hormone antibody [EPR20253-236] - BSA and Azide free (Detector) ab244971
- Anti-Parathyroid Hormone antibody [EPR20253-236] - BSA and Azide free (Detector) Anti-Parathyroid Hormone antibody [EPR20253-236] - BSA and Azide free (Detector) ab244971
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.
- Biological function summary
PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.
- Pathways
PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.
- Associated diseases and disorders
PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.
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4 product images
ELISA - Human PTH ELISA Kit (ab230931)
Sandwich ELISA - Human PTH ELISA Kit (ab230931)
Example of Human PTH standard curve in Sample Diluent NS.
Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PTH ELISA Kit (ab230931)
Interpolated concentrations of spiked PTH in Human serum, plasma and cell culture media samples.
The concentrations of PTH were measured in duplicate, interpolated from the PTH standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, plasma (heparin) 50% and 10F RPMI media 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean PTH concentration was determined to be 262.33 pg/mL in serum, 258.51 pg/mL in plasma (citrate) and 264.22 pg/mL in plasma (EDTA), 249.32 pg/mL in plasma (heparin), and 300.25 pg/mL in 10F RPMI media 100%.
Sandwich ELISA - Human PTH ELISA Kit (ab230931)
Example of Human PTH standard curve in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.
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