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AB314375

Human PTPN12 ELISA Kit

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Human PTPN12 ELISA Kit is a Sandwich ELISA for the measurement of Human PTPN12 in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

Tyrosine-protein phosphatase non-receptor type 12, PTP-PEST, Protein-tyrosine phosphatase G1, PTPG1, PTPN12

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Sandwich ELISA - Human PTPN12 ELISA Kit (AB314375)
  • sELISA

Supplier Data

Sandwich ELISA - Human PTPN12 ELISA Kit (AB314375)

Example of standard curve using ab314375. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Serum, Plasma, Cell culture supernatant

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 1.65 ng/mL

Range

1.65 - 125 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB289867

Anti-PTPN12 antibody [EPR25171-63] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-PTPN12 antibody [EPR25171-63] - BSA and Azide free (AB289867)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human PTPN12 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human PTPN12 in serum, plasma and cell culture supernatants. This assay employs an antibody specific for human PTPN12 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "120 - 136 %", "average": "= 128.7" }, { "sample": "Plasma", "range": "82 - 128 %", "average": "= 105.4" }, { "sample": "Cell culture media", "range": "105 - 113 %", "average": "= 108.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314375-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"Human PTPN12 Antibody-coated ELISA Plate", "number":"AB314375-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human PTPN12 Detection Antibody", "number":"AB314375-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314375-CMP07", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314375-CMP06", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human PTPN12 Protein Standard", "number":"AB314375-CMP10", "productcode":"" }, { "size":"1 x 200 µL", "name":"1000X HRP-Streptavidin", "number":"AB314375-CMP03", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314375-CMP05", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314375-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Protein Tyrosine Phosphatase Non-Receptor Type 12 (PTPN12) often referred to as PTP-PeST is an enzyme that plays a significant mechanical role within cells. PTPN12 is part of the protein tyrosine phosphatase (PTP) family which is responsible for removing phosphate groups from phosphotyrosine residues on proteins. This activity regulates many cellular processes including growth and differentiation. PTPN12 has a molecular mass of approximately 86 kDa. It is ubiquitously expressed in various tissues but shows higher expression in hematopoietic cells.
Biological function summary

As a non-receptor type phosphatase PTPN12 partners with several proteins to perform essential cellular functions. It interacts with multiple components in the cell participating in the assembly of protein complexes that manage cytoskeletal dynamics and cell movement. PTPN12 influences signal transduction pathways by interacting with adapter proteins such as p130Cas and Paxillin which are vital for cell adhesion and migration. It also plays a role in maintaining cellular homeostasis by controlling signaling cascades that prevent excessive cell proliferation.

Pathways

PTPN12 takes part in critical signal transduction networks most notably the Integrin signaling and RhoA pathways. In the Integrin pathway PTPN12 negatively regulates signaling by dephosphorylating focal adhesion-associated proteins. This regulation affects cell attachment to the extracellular matrix and cell motility. The interaction of PTPN12 with proteins such as Src and FAK (Focal Adhesion Kinase) illustrates its involvement in these processes. In the RhoA pathway PTPN12 influences cytoskeletal rearrangement and cell shape regulation linking to its role in cell migration.

Disruptions in PTPN12 function associate with cancer progression such as breast cancer and inflammatory disorders. In breast cancer physiological studies indicate that PTPN12 acts as a tumor suppressor. Its loss or reduced activity may lead to unregulated cell proliferation and metastasis by failing to restrain pathways involving Src and FAK. In inflammatory disorders altered activities of PTPN12 can affect immune cell function contributing to conditions like rheumatoid arthritis. The enzyme's dysfunction can amplify inflammatory signals through faults in the regulation of immune cells linking PTPN12 to increased disease susceptibility.
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Product protocols

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Target data

Dephosphorylates a range of proteins, and thereby regulates cellular signaling cascades (PubMed : 18559503). Dephosphorylates cellular tyrosine kinases, such as ERBB2 and PTK2B/PYK2, and thereby regulates signaling via ERBB2 and PTK2B/PYK2 (PubMed : 17329398, PubMed : 27134172). Selectively dephosphorylates ERBB2 phosphorylated at 'Tyr-1112', 'Tyr-1196', and/or 'Tyr-1248' (PubMed : 27134172).
See full target information PTPN12
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Product promise

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