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AB313929

Human Pyrophosphatase 1 ELISA Kit

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Human Pyrophosphatase 1 ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human Pyrophosphatase 1 in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

IOPPP, PP, PPA1, Inorganic pyrophosphatase, Pyrophosphate phospho-hydrolase, PPase

1 Images
Sandwich ELISA - Human Pyrophosphatase 1 ELISA Kit (AB313929)
  • sELISA

Supplier Data

Sandwich ELISA - Human Pyrophosphatase 1 ELISA Kit (AB313929)

Example of standard curve using ab313929. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 2.46 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human Pyrophosphatase 1 ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Pyrophosphatase 1 in serum (human Pyrophosphatase 1 concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human Pyrophosphatase 1 coated on a 96-well plate.
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Recovery

[ { "sample": "Serum", "range": "80 - 96 %", "average": "= 87.28" }, { "sample": "Plasma", "range": "70 - 97 %", "average": "= 79.6" }, { "sample": "Cell culture media", "range": "82 - 99 %", "average": "= 86.41" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Pyrophosphatase 1 also known as iPPase or inorganic pyrophosphatase 1 is an enzyme responsible for the hydrolysis of inorganic pyrophosphate (PPi) into two phosphate ions. This reaction is energetically favorable and provides the driving force for many biosynthetic processes. Pyrophosphatase 1 is a soluble cytosolic enzyme with a molecular mass of about 33 kDa. It is expressed in various tissues especially high in metabolically active cells like those found in the liver and kidney.
Biological function summary

Pyrophosphate hydrolysis by pyrophosphatase 1 plays a critical role in energy metabolism and phosphate recycling within cells. This enzyme functions independently and is not part of a larger complex. Its activity maintains the balance of phosphate compounds in the cytosol. This balance is important for cellular functions like DNA and RNA synthesis where the removal of PPi is necessary to drive these reactions forward.

Pathways

Pyrophosphatase 1 influences several key metabolic routes such as nucleotide and protein biosynthesis. The enzyme ensures the continuation of these pathways by keeping PPi concentrations low which is important for reactions like aminoacyl-tRNA synthetase activity. Pyrophosphatase 1 connects with enzymes like polymerases and ligases through these pathways facilitating a range of anabolic processes by removing inhibitory PPi byproducts.

The dysregulation of pyrophosphatase 1 can impact conditions like hypophosphatasia and some forms of arthritis. In hypophosphatasia defective function of alkaline phosphatase leads to inadequate pyrophosphate breakdown affecting bone mineralization. Pyrophosphatase 1 by regulating PPi indirectly influences the symptoms when phosphate balance is disrupted. Furthermore its role in phosphate metabolism associates pyrophosphatase 1 with pathways involving matrix proteins that are implicated in mineralization disorders.
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Product protocols

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Target data

See full target information PPA1
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websiteProtocolBooklet
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Complementary Products

Primary Antibodies

AB175223

Anti-Pyrophosphatase 1 antibody [EPR11281]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Pyrophosphatase 1 antibody [EPR11281] (AB175223)

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Proteins & Peptides

AB123186

Recombinant Human Pyrophosphatase 1 protein

SDS-PAGE - Recombinant Human Pyrophosphatase 1 protein (AB123186)

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