Human PYY ELISA Kit
- Recombinant
- SimpleStep
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(1 Publication)
Human PYY ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PYY with a sensitivity of 9.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
View Alternative Names
Peptide YY, PYY, PYY-I, Peptide tyrosine tyrosine
- sELISA
Supplier Data
Sandwich ELISA - Human PYY ELISA Kit (AB255727)
The PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
- sELISA
Supplier Data
Sandwich ELISA - Human PYY ELISA Kit (AB255727)
Representative image of Human PYY ELISA Kit (ab255727)
Components shown from left to right :
- 10X Wash Buffer PT (ab206977)
- 10X Human PYY Capture Antibody
- 10X Human PYY Detector Antibody
- Human PYY Lyophilised Purified Protein (2 vials)
- 50X Cell Extraction Enhancer Solution (ab193971)
- Sample diluent NS
- TMB solution
- Stop Solution
- 5X Cell Extraction Buffer PTR (ab193970)
- Antibody diluent 5BI
SimpleStep Pre-Coated 96-Well Microplate (ab206978) - shown behind bottles / vials.
Plate Seals - shown underneath bottles / vials.
Note : The vial labels shown in this image use generic names for illustrative purposes only and may not exactly match the specific component names included in the ELISA kit.
Note : Colors of solutions in image may not precisely match the shade of colors in the actual kit
- sELISA
Supplier Data
Sandwich ELISA - Human PYY ELISA Kit (AB255727)
The concentrations of PYY were measured in duplicate and interpolated from the PYY standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Undiluted samples are as follows : small intestine extract 37.5 μg/mL and colon extract 150 μg/mL.
- sELISA
Supplier Data
Sandwich ELISA - Human PYY ELISA Kit (AB255727)
The PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Reactivity data
Product details
Human PYY ELISA Kit (ab255727) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PYY protein in tissue extracts and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PYY with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Peptide PYY, also known as peptide tyrosine tyrosine, is a short 36- amino acid peptide encoded by the gene *PYY*. PYY is released from L cells in the ileum and colon in response to feeding, and functions by binding to NPY receptors to induce satiety. Two forms of PYY have been identified, PYY1-36 and PYY3-36, with the latter is most common form. PYY is cleaved at the Pro-30 residue by prolyl endopeptidase FAP(Separase) to generate peptide PYY3-36. PYY3-36 binds to the Y2 receptor of the Y family of receptors. PYY inhibits exocrine pancreatic secretion by vasoconstrictor action and inhibits jejunal/colonic mobility, allowing a more efficient digestion and nutrient absorption.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Recovery
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Properties and storage information
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The hormone influences several physiological functions beyond its mechanical roles. Peptide YY hormone function includes inhibiting appetite through interactions with the neuropeptide Y (NPY) receptors in the brain's hypothalamus. It acts together with similar peptides including pancreatic polypeptide (PP) and neuropeptide Y (NPY) establishing a complex regulatory system that governs energy balance and food intake. PYY contributes to short-term and long-term body weight management linking gut to brain signals.
Pathways
Peptide YY hormone function integrates into the gut-brain axis and the neuroendocrine system. It engages mainly in the regulation of the Y2 receptor pathway interacting with proteins like the ghrelin receptor and insulin pathways. Moreover PYY can influence the feeding pathway by modulating the arcuate nucleus neurons establishing connections with the melanocortin system and agouti-related peptide (AgRP). This integration importantly contributes to satiety and appetite modulation.
Product protocols
- Visit the General protocols
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- Download websiteProtocolBooklet|en
Target data
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Frontiers in physiology 13:827335 PubMed35264977
2022
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