Human PYY ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PYY with a sensitivity of 9.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
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- Frontiers in physiology 13:8273352022Effects of Three Different Modes of Resistance Training on Appetite Hormones in Males With Obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAli Ataeinosrat,Marjan Mosalman Haghighi,Hossein Abednatanzi,Mohammad Soltani,Abbass Ghanbari-Niaki,Akbar Nouri-Habashi,Sadegh Amani-Shalamzari,Ali Mossayebi,Mitra Khademosharie,Kelly E Johnson,Trisha A VanDusseldorp,Ayoub Saeidi,Hassane ZouhalPubMed 35264977
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 1000 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 9.8 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Alternative names
Peptide YY, PYY, PYY-I, Peptide tyrosine tyrosine
What's included?
Recommended products
Human PYY ELISA Kit is a single-wash 90-min Simplestep used to quantify Human PYY with a sensitivity of 9.8 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Tissue Extracts, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 31.25 - 1000 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 9.8 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 8 | mean - | SD - | C.V. 11.7 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Extract | n 3 | mean - | SD - | C.V. 12.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type EDTA Plasma | Average % = 92 | Range 81 - 106 % |
Sample type Tissue Extracts | Average % = 109 | Range 108 - 111 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human PYY ELISA Kit (ab255727) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of PYY protein in tissue extracts and edta plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human PYY with 8.4 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Peptide PYY, also known as peptide tyrosine tyrosine, is a short 36- amino acid peptide encoded by the gene *PYY*. PYY is released from L cells in the ileum and colon in response to feeding, and functions by binding to NPY receptors to induce satiety. Two forms of PYY have been identified, PYY1-36 and PYY3-36, with the latter is most common form. PYY is cleaved at the Pro-30 residue by prolyl endopeptidase FAP(Separase) to generate peptide PYY3-36. PYY3-36 binds to the Y2 receptor of the Y family of receptors. PYY inhibits exocrine pancreatic secretion by vasoconstrictor action and inhibits jejunal/colonic mobility, allowing a more efficient digestion and nutrient absorption.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Peptide YY (PYY) also known as PYY1 or PYY3-36 is a 36-amino acid peptide hormone with a molecular weight of approximately 4 kDa. Expressed primarily in the L-cells of the ileum and colon PYY is released into the bloodstream postprandially. This hormone serves as an important messenger in appetite control and energy homeostasis. Peptide YY reduces gastric motility and increases water and electrolyte absorption in the colon playing an integral role in digestion and nutrient absorption processes.
- Biological function summary
The hormone influences several physiological functions beyond its mechanical roles. Peptide YY hormone function includes inhibiting appetite through interactions with the neuropeptide Y (NPY) receptors in the brain's hypothalamus. It acts together with similar peptides including pancreatic polypeptide (PP) and neuropeptide Y (NPY) establishing a complex regulatory system that governs energy balance and food intake. PYY contributes to short-term and long-term body weight management linking gut to brain signals.
- Pathways
Peptide YY hormone function integrates into the gut-brain axis and the neuroendocrine system. It engages mainly in the regulation of the Y2 receptor pathway interacting with proteins like the ghrelin receptor and insulin pathways. Moreover PYY can influence the feeding pathway by modulating the arcuate nucleus neurons establishing connections with the melanocortin system and agouti-related peptide (AgRP). This integration importantly contributes to satiety and appetite modulation.
- Associated diseases and disorders
Peptide YY is linked to obesity and irritable bowel syndrome (IBS). Altered levels of PYY associate with increased risk of obesity due to dysregulated appetite signals. Disorders like IBS relate to the imbalance of functional gut hormones including PYY which impacts bowel motility and gastrointestinal comfort. Other proteins such as ghrelin and leptin participate in these pathways providing insight into potential therapeutic targets for these conditions.
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3 product images
Sandwich ELISA - Human PYY ELISA Kit (ab255727)
The PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human PYY ELISA Kit (ab255727)
The concentrations of PYY were measured in duplicate and interpolated from the PYY standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Undiluted samples are as follows: small intestine extract 37.5 μg/mL and colon extract 150 μg/mL.
Sandwich ELISA - Human PYY ELISA Kit (ab255727)
The PYY standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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