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AB284020

Human QPRT ELISA Kit

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Human QPRT ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human QPRT in Human in Biofluids, Cell/Tissue Extracts samples.

View Alternative Names

Nicotinate-nucleotide pyrophosphorylase [carboxylating], Quinolinate phosphoribosyltransferase [decarboxylating], QAPRTase, QPRTase, QPRT

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Sandwich ELISA - Human QPRT ELISA Kit (AB284020)
  • sELISA

Supplier Data

Sandwich ELISA - Human QPRT ELISA Kit (AB284020)

Example of Human QPRT standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cerebral Spinal Fluid, Saliva, Urine, Plasma, Cell Lysate, Milk, Serum, Tissue

Reacts with

Human

Assay type

Sandwich (quantitative)

Sensitivity

= 0.25 ng/mL

Range

1.563 - 12.5 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Quinolinate phosphoribosyltransferase (QPRT) belongs to the phosphoribosyltransferase family and is involved in de novo NAD biosynthesis using quinolinate as a precursor. Quinolinate acts as a most potent endogenous exitotoxin to neurons, causing neuronal damage through sustained activation of glutamate N-methyl-D-aspartate receptors.

Human QPRT ELISA (Enzyme-Linked Immunosorbent Assay) Kit is designed for detection of QPRT in human plasma, serum, milk, urine, saliva, CSF, and cell culture samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human QPRT in approximately 4 hours. A polyclonal antibody specific for human QPRT has been pre-coated onto a 96-well microplate with removable strips. QPRT in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human QPRT, which is recognized by a streptavidin-peroxidase (SP) conjugate. The color development is stopped and the intensity of the color is measured.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.5" } ]
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What's included?

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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

QPRT also known as Nicotinate-nucleotide pyrophosphorylase [carboxylating] or Quinolinate phosphoribosyltransferase functions as an important enzyme in the kynurenine pathway a metabolic pathway leading to the production of nicotinamide adenine dinucleotide (NAD+). The enzyme catalyzes the conversion of quinolinate to nicotinic acid mononucleotide. QPRT has a molecular mass of approximately 34 kDa. It is expressed in various tissues most notably in the liver kidney and brain.
Biological function summary

QPRT influences the synthesis of NAD+ which is essential for cellular redox reactions and energy metabolism. The enzyme operates as a monomer and does not typically form part of a larger complex. However its activity is important in maintaining NAD+ levels which can impact a wide range of cellular processes such as DNA repair signal transduction and metabolism.

Pathways

QPRT holds a significant role in the kynurenine pathway and NAD+ biosynthesis. This pathway includes several enzymes such as kynurenine 3-monooxygenase and kynureninase which collaboratively contribute to the degradation of tryptophan ultimately affecting the levels of important coenzymes and neurotransmitters. The NAD+ biosynthesis pathway involving QPRT also interacts with pathways for ATP generation connecting with other metabolic processes.

QPRT's malfunction links to neurological disorders such as Alzheimer's disease due to its role in NAD+ metabolism and potential effects on neuronal health and energy supply. Additionally alterations in QPRT activity have associations with liver diseases considering its expression and function in metabolic pathways within hepatic tissues. Interaction with proteins like kynurenine aminotransferase highlights its involvement in maintaining metabolic balance within these conditions.
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Product protocols

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Target data

Involved in the catabolism of quinolinic acid (QA).
See full target information Nicotinate-nucleotide pyrophosphorylase [carboxylating]
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websiteProtocolBooklet
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