JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB283971

Human Quinine oxidoreductase ELISA Kit

Be the first to review this product! Submit a review

|

(0 Publication)

Human Quinine oxidoreductase ELISA Kit is a Sandwich ELISA for the measurement of Human Quinine oxidoreductase in Human in Biofluids, Cell Culture Media, Cell/Tissue Extracts samples.

View Alternative Names

Zeta-crystallin, NADPH:quinone reductase, Quinone oxidoreductase, CRYZ

1 Images
Sandwich ELISA - Human Quinine oxidoreductase ELISA Kit (AB283971)
  • sELISA

Supplier Data

Sandwich ELISA - Human Quinine oxidoreductase ELISA Kit (AB283971)

Example of Human Quinine oxidoreductase standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Saliva, Urine, Plasma, Cell culture supernatant, Milk, Serum, Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 25 pg/mL

Range

0.037 - 2.4 ng/mL

Assay time

4h

Assay Platform

Microplate (12 x 8 well strips)

style
left-column

Complementary Products

Assay Kits

AB187391

Human Transferrin ELISA Kit

SimpleStep

ELISA - Human Transferrin ELISA Kit (AB187391)

  • 0
  • 0
Assay Kits

AB272202

Human P-Selectin (CD62P) ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human P-Selectin (CD62P) ELISA Kit (AB272202)

  • 0
  • 0
Assay Kits

AB238266

Human Uteroglobin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Uteroglobin ELISA Kit (AB238266)

  • 0
  • 0
Assay Kits

AB137988

Human Protein C ELISA Kit

Competitive ELISA - Human Protein C ELISA Kit (AB137988)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

The Human Quinine oxidoreductase ELISA Kit (ab283971) is designed for detection of Quinine oxidoreductase in human plasma, serum, milk, urine, saliva, cell culture, and cell lysate samples. This assay employs a quantitative sandwich enzyme immunoassay technique that measures human Quinine oxidoreductase in approximately 4 hours.

A polyclonal antibody specific for human quinine oxidoreductase has been pre-coated onto a 96-well microplate with removable strips. Quinine oxidoreductase in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human quinine oxidoreductase, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

style
left-column

Precision

[ { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.1" }, { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 60, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5.7" } ]
style
left-column

What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab283971-1x96Tests", "assetComponentDetails": [ { "size":"2 x 30 mL", "name":"20X Wash Buffer Concentrate", "number":"AB283971-CMP05", "productcode":"" }, { "size":"1 x 11 mL", "name":"Stop Solution", "number":"AB283971-CMP09", "productcode":"" }, { "size":"1 x 7 mL", "name":"Chromogen Substrate", "number":"AB283971-CMP04", "productcode":"" }, { "size":"1 x 120 µL", "name":"50X Biotinylated Human Quinine oxidoreductase Antibody", "number":"AB283971-CMP06", "productcode":"" }, { "size":"3 x 1 Unit", "name":"Sealing Tapes", "number":"AB283971-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"10X Diluent N Concentrate", "number":"AB283971-CMP01", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human Quinine oxidoreductase Microplate", "number":"AB283971-CMP03", "productcode":"" }, { "size":"1 x 80 µL", "name":"100X Streptavidin-Peroxidase Conjugate", "number":"AB283971-CMP10", "productcode":"" }, { "size":"1 x 2 mL", "name":"1X Standard Diluent", "number":"AB283971-CMP02", "productcode":"" }, { "size":"1 x 1 Vial", "name":"Quinine oxidoreductase Standard", "number":"AB283971-CMP07", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
Multi
Storage information
Please refer to protocols
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Quinone oxidoreductase also known by its popular alternate name NQO1 is a flavoenzyme with a molecular mass of approximately 31 kDa. It functions mechanically by catalyzing the reduction of quinones to hydroquinones utilizing NAD(P)H as an electron donor. This enzymatic activity helps in preventing redox cycling and oxidative stress by removing reactive quinones. Quinone oxidoreductase is expressed in various tissues including liver lung and heart highlighting its role in many biological processes.
Biological function summary

Quinone oxidoreductase acts as a detoxifying enzyme. It maintains cellular protection against oxidative damage by neutralizing harmful quinones. This enzyme is not reported as part of a larger protein complex but it plays a complementary role with other phase II detoxification enzymes like glutathione S-transferase. Its protective attributes attribute to cell survival especially under oxidative stress conditions.

Pathways

Quinone oxidoreductase is integral to the oxidative stress response and xenobiotic metabolism pathways. It is closely related to proteins such as cytochrome P450 and glutathione which work together to maintain cellular redox homeostasis and metabolism of foreign compounds. This collaboration allows quinone oxidoreductase to help in converting toxic substances into less harmful forms.

Quinone oxidoreductase is implicated in cancer and neurodegenerative disorders. In cancer its overexpression can lead to chemoresistance as its detoxifying functions may neutralize chemotherapeutic agents sharing functional pathways with proteins like p53. In neurodegenerative diseases altered activity of quinone oxidoreductase influences oxidative damage a common cause in disorders such as Parkinson's disease. Its relation with proteins like PARK7 (DJ-1) highlights its role in protecting neuronal cells from oxidative stress.
style
left-column

Product protocols

style
left-column

Target data

Multifunctional crystallin endowed with unrelated mRNA stabilization and enzymatic oxidoreductase activities (PubMed : 17497241, PubMed : 20103721). Binds (AU)-rich elements (ARE) in the 3'-UTR of target mRNA to enhance their stability (PubMed : 17497241, PubMed : 20103721). Upon metabolic acidosis, stabilizes the mRNA encoding SLC12A1 a cotransporter involved in transepithelial NH4(+) reabsorption in the medullary thick ascending limb in kidney (By similarity). In response to acidosis, also participates to the adaptive increase in renal ammoniagenesis by stabilizing the mRNAs of enzymes involved in glutamine catabolism (By similarity). By the same mechanism, it also regulates the expression of the antiapoptotic protein BCL2 (PubMed : 20103721). Beside its mRNA stabilization activity, also catalyzes the reduction of orthoquinones such as 1,2-naphthoquinone or 9,10-phenanthrenequinone in the presence of NADPH in vitro and could be involved in the detoxification of xenobiotics and protection against oxidative stress (PubMed : 17497241).
See full target information Quinone oxidoreductase
style
left-column
style
left-column
websiteProtocolBooklet
en
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com