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AB314718

Human RAET1E ELISA Kit

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Human RAET1E ELISA Kit is a Sandwich ELISA for the measurement of Human RAET1E in Human in Cell Culture Media, Biofluids samples.

View Alternative Names

LETAL, N2DL4, ULBP4, UNQ1867/PRO4303, RAET1E, Retinoic acid early transcript 1E, Lymphocyte effector toxicity activation ligand, NKG2D ligand 4, RAE-1-like transcript 4, UL16-binding protein 4, N2DL-4, NKG2DL4

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Sandwich ELISA - Human RAET1E ELISA Kit (AB314718)
  • sELISA

Supplier Data

Sandwich ELISA - Human RAET1E ELISA Kit (AB314718)

Example of standard curve using ab314718. This standard curve is for demonstrative purposes only. A standard curve must be run with each assay.

Key facts

Detection method

Colorimetric

Sample types

Cell culture supernatant, Plasma, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.43 ng/mL

Range

0.43 - 100 ng/mL

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB200662

Anti-RAET1E antibody [EPR17443]

RabMAb|Recombinant

Immunoprecipitation - Anti-RAET1E antibody [EPR17443] (AB200662)

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Proteins & Peptides

AB137171

Recombinant Human RAET1E protein (denatured) (His tag N-Terminus)

SDS-PAGE - Recombinant Human RAET1E protein (denatured) (His tag N-Terminus) (AB137171)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RAET1E ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RAET1E in serum (human RAET1E concentration is low in normal serum/plasma, and may not be detectable in this assay), plasma and cell culture supernatants. This assay employs an antibody specific for human RAET1E coated on a 96-well plate.

This ELISA kit shows no cross-reactivity with the following cytokines tested: human Coagulation Factor X, CHMP2B, Contactin-3, Cortactin, CrkL, Cyr61/CCN1, DAPP1, Dynactin Subunit 1/DCTN1, DFF45/ICAD, DRAK1, GRAP2, GRK5, Hydroxyacid Oxidase1/HAO-1, LRIG1, MMP-12, NCK1, Nectin-2/CD112, Nesfatin1/Nucleobindin-2, Neurogranin, Nrf2, NUDT5, Nucleoporin NUP85, PAR1, Pancreatic Polypeptide/PP, Peroxiredoxin 2, PSMA1, PU.1/Spi-1, RalA, RCOR1/CoREST, Serpin B8, SH2D1A, SHP-1, Siglec-6/CD327, SorCS3, THAP11, UNC5H3, VAMP-1, VAMP-2, PBEF/Visfatin

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Recovery

[ { "sample": "Serum", "range": "87 - 146 %", "average": "= 119.4" }, { "sample": "Plasma", "range": "70 - 94 %", "average": "= 79.09" }, { "sample": "Cell culture media", "range": "120 - 145 %", "average": "= 134.2" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab314718-1x96Tests", "assetComponentDetails": [ { "size":"1 x 15 mL", "name":"5X Assay Diluent (Item E2)", "number":"AB314718-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Human RAET1E Antibody-coated ELISA Plate", "number":"AB314718-CMP08", "productcode":"" }, { "size":"1 x 200 µL", "name":"600X HRP-Streptavidin", "number":"AB314718-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution; 0.2M Sulfuric Acid", "number":"AB314718-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB314718-CMP07", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human RAET1E Detection Antibody", "number":"AB314718-CMP09", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Lyophilized Human RAET1E Protein Standard", "number":"AB314718-CMP10", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB314718-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RAET1E also known as UL16-binding protein 4 (ULBP4) is a member of the MHC class I-related molecules. It has a molecular mass of approximately 31 kDa. RAET1E is part of the family of proteins that acts as a ligand for the NKG2D receptor found on the surface of natural killer (NK) cells. In terms of expression RAET1E is present on the surface of various cell types especially in stressed transformed or virus-infected cells where it signals cellular distress to immune cells.
Biological function summary

RAET1E plays a role in immune surveillance. It is involved in the activation of NK cells when it binds to the NKG2D receptor. This interaction is part of the body's defense mechanism against transformed or infected cells. RAET1E operates in isolation rather than as part of a larger protein complex which allows it to independently initiate the immune response when detecting harmful cells.

Pathways

RAET1E functions within the innate immune response pathway. It works closely with the NKG2D signaling pathway which is important for the activation of cytotoxic activities in immune cells. RAET1E through its binding to NKG2D facilitates the destruction of malignant cells. This connection places RAET1E alongside other similar NKG2D ligands such as MIC and other ULBP family proteins which contribute to the activation of NK and T cells.

RAET1E is linked to various cancer types and infectious diseases. Its expression on the surface of tumor cells can signal NK cells to target and destroy these potentially harmful cells. In certain cancers the loss or reduction of RAET1E expression may allow tumor cells to escape immune detection. In viral infections the upregulation of RAET1E on infected cells assists in the clearance of viruses by NK cells. Both in cancer and viral infections proteins like RAET1E and NKG2D play an important part in the immune system's defensive strategies.
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Product protocols

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Target data

Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
See full target information RAET1E
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websiteProtocolBooklet
en
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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