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AB309182

Human RALBP1 ELISA Kit

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Human RALBP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human RALBP1 with a sensitivity of 27.139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair

View Alternative Names

RLIP, RLIP1, RLIP76, RALBP1, RalA-binding protein 1, RalBP1, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, Ral-interacting protein 1, DNP-SG ATPase

2 Images
Sandwich ELISA - Human RALBP1 ELISA Kit (AB309182)
  • sELISA

Supplier Data

Sandwich ELISA - Human RALBP1 ELISA Kit (AB309182)

Interpolated concentration of native RALBP1 was measured in duplicate at different sample concentrations. Undiluted samples are 500 μg/mL MCF-7 extract and Jurkat extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.

Sandwich ELISA - Human RALBP1 ELISA Kit (AB309182)
  • sELISA

Supplier Data

Sandwich ELISA - Human RALBP1 ELISA Kit (AB309182)

Example of human RALBP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Lysate

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 27.139 pg/mL

Range

132.813 - 8500 pg/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Complementary Products

Primary Antibodies

AB248548

Anti-RALBP1 antibody [EPR6472] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Western blot - Anti-RALBP1 antibody [EPR6472] - BSA and Azide free (AB248548)

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Cell Lines & Lysates

AB258167

Human RALBP1 knockout HeLa cell lysate

Western blot - Human RALBP1 knockout HeLa cell lysate (AB258167)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RALBP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RALBP1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human RALBP1 with 27.139 pg/ml sensitivity.

SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:

-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips

A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "8.5" }, { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "10.2" } ]
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Recovery

[ { "sample": "Cell Lysate", "range": "80 - 87 %", "average": "= 83" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab309182-1x96Tests", "assetComponentDetails": [ { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB309182-CMP10", "productcode":"" }, { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB309182-CMP09", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB309182-CMP06", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB309182-CMP08", "productcode":"" }, { "size":"1 x 12 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB309182-CMP07", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RALBP1 Capture Antibody", "number":"AB309182-CMP01", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent 4BR", "number":"AB309182-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human RALBP1 Lyophilized Recombinant Protein", "number":"AB309182-CMP03", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB309182-CMP11", "productcode":"" }, { "size":"1 x 10 mL", "name":"5X Cell Extraction Buffer PTR (ab193970)", "number":"AB309182-CMP05", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RALBP1 Detector Antibody", "number":"AB309182-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RALBP1 also known as ralA binding protein 1 or RLIP76 is a multifunctional protein involved in various cellular processes. It has a molecular mass of approximately 76 kDa. RALBP1 often associates with cell membranes and is expressed ubiquitously in different tissues indicating its widespread role in cellular functioning. The protein interacts with small GTPases such as RalA playing critical roles in signal transduction processes.
Biological function summary

RALBP1 serves as an effector protein involved in several cellular activities including endocytosis and transport of glutathione conjugates. It functions as part of a complex with partner proteins facilitating the movement of substrates across cellular membranes. This role is essential for maintaining cellular homeostasis as it helps to eliminate toxic compounds and regulate intracellular signals.

Pathways

RALBP1 participates in the Rho family small GTPase signaling and the Ras signaling pathways. Within these pathways the protein coordinates with RalA and other GTPases to control the trafficking of vesicles and stress response mechanisms. Through these interactions RALBP1 plays an important role in cytoskeletal reorganization and cellular response to extracellular cues.

RALBP1 has associations with cancer and metabolic conditions. Alterations in its function or expression levels may contribute to oncogenic processes as RALBP1 interacts with factors like the epidermal growth factor receptor (EGFR) which is often dysregulated in cancerous cells. Moreover its role in detoxification processes implicates RALBP1 in metabolic disorders potentially affecting the body's capability to manage oxidative stress.
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Product protocols

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Target data

Multifunctional protein that functions as a downstream effector of RALA and RALB (PubMed : 7673236). As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity (PubMed : 7673236). As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis (PubMed : 10910768, PubMed : 12775724). During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle (PubMed : 12775724). During mitosis, also controls mitochondrial fission as an effector of RALA (PubMed : 21822277). Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L (PubMed : 21822277).. Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
See full target information RALBP1
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Product promise

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