Human RALBP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human RALBP1 with a sensitivity of 27.139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Images
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 132.813 - 8500 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 27.139 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Multifunctional protein that functions as a downstream effector of RALA and RALB (PubMed:7673236). As a GTPase-activating protein/GAP can inactivate CDC42 and RAC1 by stimulating their GTPase activity (PubMed:7673236). As part of the Ral signaling pathway, may also regulate ligand-dependent EGF and insulin receptors-mediated endocytosis (PubMed:10910768, PubMed:12775724). During mitosis, may act as a scaffold protein in the phosphorylation of EPSIN/EPN1 by the mitotic kinase cyclin B-CDK1, preventing endocytosis during that phase of the cell cycle (PubMed:12775724). During mitosis, also controls mitochondrial fission as an effector of RALA (PubMed:21822277). Recruited to mitochondrion by RALA, acts as a scaffold to foster the mitotic kinase cyclin B-CDK1-mediated phosphorylation and activation of DNM1L (PubMed:21822277). Could also function as a primary ATP-dependent active transporter for glutathione conjugates of electrophiles. May also actively catalyze the efflux of a wide range of substrates including xenobiotics like doxorubicin (DOX) contributing to cell multidrug resistance.
Alternative names
RLIP, RLIP1, RLIP76, RALBP1, RalA-binding protein 1, RalBP1, 76 kDa Ral-interacting protein, Dinitrophenyl S-glutathione ATPase, Ral-interacting protein 1, DNP-SG ATPase
What's included?
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Human RALBP1 ELISA Kit is a single-wash 90-min Simplestep used to quantify Human RALBP1 with a sensitivity of 27.139 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
Key facts
- Detection method
- Colorimetric
- Sample types
- Cell Lysate
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 132.813 - 8500 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Pre-coated microplate (12 x 8 well strips)
- Sensitivity
- = 27.139 pg/mL
- General recovery
- 83%
Precision
Intra assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 8 | C.V. 8.5 |
Inter assay
| Sample | n | C.V. |
|---|---|---|
Sample Extract | n 3 | C.V. 10.2 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell Lysate | Average % = 83 | Range 80 - 87 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human RALBP1 SimpleStep ELISA® kit is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RALBP1 protein in human serum, plasma, saliva and cell culture supernatant. Quantitate Human RALBP1 with 27.139 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
RALBP1 also known as ralA binding protein 1 or RLIP76 is a multifunctional protein involved in various cellular processes. It has a molecular mass of approximately 76 kDa. RALBP1 often associates with cell membranes and is expressed ubiquitously in different tissues indicating its widespread role in cellular functioning. The protein interacts with small GTPases such as RalA playing critical roles in signal transduction processes.
- Biological function summary
RALBP1 serves as an effector protein involved in several cellular activities including endocytosis and transport of glutathione conjugates. It functions as part of a complex with partner proteins facilitating the movement of substrates across cellular membranes. This role is essential for maintaining cellular homeostasis as it helps to eliminate toxic compounds and regulate intracellular signals.
- Pathways
RALBP1 participates in the Rho family small GTPase signaling and the Ras signaling pathways. Within these pathways the protein coordinates with RalA and other GTPases to control the trafficking of vesicles and stress response mechanisms. Through these interactions RALBP1 plays an important role in cytoskeletal reorganization and cellular response to extracellular cues.
- Associated diseases and disorders
RALBP1 has associations with cancer and metabolic conditions. Alterations in its function or expression levels may contribute to oncogenic processes as RALBP1 interacts with factors like the epidermal growth factor receptor (EGFR) which is often dysregulated in cancerous cells. Moreover its role in detoxification processes implicates RALBP1 in metabolic disorders potentially affecting the body's capability to manage oxidative stress.
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2 product images
Sandwich ELISA - Human RALBP1 ELISA Kit (ab309182)
Example of human RALBP1 standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human RALBP1 ELISA Kit (ab309182)
Interpolated concentration of native RALBP1 was measured in duplicate at different sample concentrations. Undiluted samples are 500 μg/mL MCF-7 extract and Jurkat extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer PTR.
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