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AB193713

Human RANK ELISA Kit

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Human RANK ELISA Kit is a Sandwich ELISA for the measurement of Human RANK in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

CD265, RANK, TNFRSF11A, Tumor necrosis factor receptor superfamily member 11A, Osteoclast differentiation factor receptor, Receptor activator of NF-KB, ODFR

1 Images
Sandwich ELISA - Human RANK ELISA Kit (AB193713)
  • sELISA

Supplier Data

Sandwich ELISA - Human RANK ELISA Kit (AB193713)

Representative Standard Curve using ab193713

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.4 ng/mL

Range

0.4 - 100 ng/mL

Assay Platform

Microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's RANK Human ELISA Kit (ab193713) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RANK in serum, plasma and cell culture supernatants.

This assay employs an antibody specific for Human RANK coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures RANK present in the samples. The wells are washed and biotinylated anti-Human RANK antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of RANK bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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Precision

[ { "reproducibilityType": "Intra", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 10" }, { "reproducibilityType": "Inter", "sample": "Overall", "replicates": 0, "mean": null, "standardDeviation": null, "coefficientOfVariability": "< 12" } ]
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Recovery

[ { "sample": "Serum", "range": "68 - 90 %", "average": "= 78.61" }, { "sample": "Plasma", "range": "69 - 87 %", "average": "= 76.13" }, { "sample": "Cell culture media", "range": "72 - 133 %", "average": "= 100.3" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab193713-1x96Tests", "assetComponentDetails": [ { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB193713-CMP08", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent C", "number":"AB193713-CMP07", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB193713-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB193713-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Pre-coated Human RANK Microplate (12 strips x 8 wells)", "number":"AB193713-CMP03", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated Human RANK detection antibody", "number":"AB193713-CMP05", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human RANK Standards (lyophilized)", "number":"AB193713-CMP04", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB193713-CMP01", "productcode":"" }, { "size":"1 x 200 µL", "name":"200X HRP-Streptavidin Concentrate", "number":"AB193713-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Receptor for TNFSF11/RANKL/TRANCE/OPGL; essential for RANKL-mediated osteoclastogenesis (PubMed : 9878548). Its interaction with EEIG1 promotes osteoclastogenesis via facilitating the transcription of NFATC1 and activation of PLCG2 (By similarity). Involved in the regulation of interactions between T-cells and dendritic cells (By similarity).
See full target information TNFRSF11A
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websiteProtocolBooklet
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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