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AB100633

Human RANTES ELISA Kit

5

(1 Review)

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(8 Publications)

Human RANTES ELISA Kit is a Sandwich ELISA for the measurement of Human RANTES in Human in Biofluids, Cell Culture Media samples.

View Alternative Names

D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228

4 Images
Sandwich ELISA - Human RANTES ELISA Kit (AB100633)
  • sELISA

Lab

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)
  • sELISA

Lab

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)

RANTES measured in cell supernatants showing quantity (pg) per mL of tested sample. Samples were diluted 1-8 fold.

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)
  • sELISA

Lab

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)

RANTES measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 6-54 fold.

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)
  • sELISA

Lab

Sandwich ELISA - Human RANTES ELISA Kit (AB100633)

Standard curve : mean of duplicates (+/- SD) with background reads subtracted

Key facts

Detection method

Colorimetric

Sample types

Plasma, Cell culture supernatant, Serum

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

< 3 pg/mL

Range

2.74 - 2000 pg/mL

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Abcam's RANTES Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human RANTES in serum, plasma, and cell culture supernatants.

This assay employs an antibody specific for Human RANTES coated on a 96-well plate. Standards and samples are pipetted into the wells and RANTES present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human RANTES antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RANTES bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Optimization may be required with urine samples.

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Recovery

[ { "sample": "Cell culture supernatant", "range": "87 - 108 %", "average": "= 102.6" }, { "sample": "Serum", "range": "85 - 104 %", "average": "= 96.74" }, { "sample": "Plasma", "range": "84 - 103 %", "average": "= 94.76" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab100633-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"TMB One-Step Substrate Reagent", "number":"AB100633-CMP09", "productcode":"" }, { "size":"1 x 15 mL", "name":"5X Assay Diluent B", "number":"AB100633-CMP07", "productcode":"" }, { "size":"1 x 30 mL", "name":"Assay Diluent A", "number":"AB100633-CMP06", "productcode":"" }, { "size":"1 x 1 Unit", "name":"RANTES Microplate (12 x 8 wells)", "number":"AB100633-CMP01", "productcode":"" }, { "size":"1 x 25 mL", "name":"20X Wash Buffer", "number":"AB100633-CMP03", "productcode":"" }, { "size":"1 x 8 mL", "name":"Stop Solution", "number":"AB100633-CMP08", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Biotinylated anti-Human RANTES", "number":"AB100633-CMP02", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Recombinant Human RANTES Standard (lyophilized)", "number":"AB100633-CMP05", "productcode":"" }, { "size":"1 x 200 µL", "name":"300X HRP-Streptavidin Concentrate", "number":"AB100633-CMP04", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
-20°C
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Product protocols

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Target data

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed : 1380064, PubMed : 15923218, PubMed : 16791620, PubMed : 8525373, PubMed : 9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed : 23979485).
See full target information CCL5
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Immunologic research 73:27 PubMed39777574

2025

Inactivation of ATG13 stimulates chronic demyelinating pathologies in muscle-serving nerves and spinal cord.

Applications

Unspecified application

Species

Unspecified reactive species

Molly E Drosen,Sarojini Bulbule,Gunnar Gottschalk,Daniel Peterson,Linda Adrienne Allen,Leggy A Arnold,Avik Roy

Malaria journal 23:335 PubMed39521981

2024

Association between RANTES/CCL5 levels with Plasmodium infections and malaria severity: a systematic review.

Applications

Unspecified application

Species

Unspecified reactive species

Pattamaporn Kwankaew,Aongart Mahittikorn,Wanida Mala,Kwuntida Uthaisar Kotepui,Nsoh Godwin Anabire,Polrat Wilairatana,Manas Kotepui

Journal of inflammation research 15:1501-1519 PubMed35261546

2022

Osthole Regulates Secretion of Pro-Inflammatory Cytokines and Expression of and in Normal Human Keratinocytes and Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Kordulewska,Justyna Topa,Anna Cieślińska,Beata Jarmołowska

Cells 11: PubMed35011650

2021

Osthole Inhibits Expression of Genes Associated with Toll-like Receptor 2 Signaling Pathway in an Organotypic 3D Skin Model of Human Epidermis with Atopic Dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Karolina Kordulewska,Justyna Topa,Robert Stryiński,Beata Jarmołowska

Journal of medical virology 93:3656-3665 PubMed32975838

2020

Prognostic, clinical, and therapeutic importance of RANTES-CCR5 axis in hepatitis A infection: A multiapproach study.

Applications

Unspecified application

Species

Unspecified reactive species

Vargab Baruah,Diptika Tiwari,Rajib Kishore Hazam,Moumita Bose,Dipankar Bujarbaruah,Anjan Kumar Saikia,Premashish Kar,Sangit Dutta,Sujoy Bose

Viruses 12: PubMed32604892

2020

HHV-6 Infection and Chemokine RANTES Signaling Pathway Disturbance in Patients with Autoimmune Thyroiditis.

Applications

Unspecified application

Species

Unspecified reactive species

Alina Sultanova,Maksims Cistjakovs,Liba Sokolovska,Katerina Todorova,Egils Cunskis,Modra Murovska

Journal of medical virology 91:2066-2073 PubMed31368534

2019

Significance of RANTES-CCR5 axis and linked downstream immunomodulation in Dengue pathogenesis: A study from Guwahati, India.

Applications

Unspecified application

Species

Unspecified reactive species

Mafidul Islam,Trishna Kalita,Anjan K Saikia,Anjuma Begum,Vargab Baruah,Nidhi Singh,Raktim Borkotoky,Sujoy Bose

Parasite immunology 39: PubMed28686299

2017

RANTES levels as a determinant of falciparum malaria severity or recovery.

Applications

Unspecified application

Species

Unspecified reactive species

D Bujarbaruah,M P Kalita,V Baruah,T K Basumatary,S Hazarika,R H Begum,S Medhi,S Bose
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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