Human RANTES ELISA Kit (CCL5) is a single-wash 90-min Simplestep used to quantify Human RANTES (CCL5) with a sensitivity of 0.091 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
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- Nature communications 14:33642023Transcriptional control of pancreatic cancer immunosuppression by metabolic enzyme CD73 in a tumor-autonomous and -autocrine manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTianyu Tang,Xing Huang,Minghao Lu,Gang Zhang,Xu Han,Tingbo LiangPubMed 37291128
- Oncology letters 25:2252023Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDan Li,Junmei Li,Feng Yu,Bo Wang,Bing LiuPubMed 37153045
- Research (Washington, D.C.) 6:00332023PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJue Tang,Jing He,Huiqin Guo,Huiran Lin,Meng Li,Tianyou Yang,Hai-Yun Wang,Di Li,Jiabin Liu,Le Li,Huimin Xia,Zhenjian Zhuo,Lei MiaoPubMed 37040518
- Nature communications 13:26922022Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonathan X Meng,Yu Zhang,Dominik Saman,Arshad M Haider,Suman De,Jason C Sang,Karen Brown,Kun Jiang,Jane Humphrey,Linda Julian,Eric Hidari,Steven F Lee,Gabriel Balmus,R Andres Floto,Clare E Bryant,Justin L P Benesch,Yu Ye,David KlenermanPubMed 35577786
- Oncology letters 22:6962021Cancer-associated fibroblast-derived CCL5 contributes to cisplatin resistance in A549 NSCLC cells partially through upregulation of lncRNA HOTAIR expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiangjun Sun,Zhijie ChenPubMed 34457051
- Molecular cancer therapeutics 19:2598-26112020Anti-S100A4 Antibody Therapy Is Efficient in Treating Aggressive Prostate Cancer and Reversing Immunosuppression: Serum and Biopsy as a Clinical Predictor.Applications:Unspecified applicationReactive species:Unspecified reactive speciesArsheed A Ganaie,Adrian P Mansini,Tabish Hussain,Arpit Rao,Hifzur R Siddique,Ashraf Shabaneh,Marina G Ferrari,Paari Murugan,Jörg Klingelhöfer,Jinhua Wang,Noona Ambartsumian,Christopher A Warlick,Badrinath R Konety,Mohammad SaleemPubMed 32999046
- Journal of extracellular vesicles 9:18011532020(Sub)populations of extracellular vesicles released by TNF-α -triggered human endothelial cells promote vascular inflammation and monocyte migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaharak Hosseinkhani et. al.PubMed 32944190
- JCI insight 5:2020Clinical and pathological investigation of patients with severe COVID-19.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaohua Li,Lina Jiang,Xi Li,Fang Lin,Yijin Wang,Boan Li,Tianjun Jiang,Weimin An,Shuhong Liu,Hongyang Liu,Pengfei Xu,Lihua Zhao,Lixin Zhang,Jinsong Mu,Hongwei Wang,Jiarui Kang,Yan Li,Lei Huang,Caizhong Zhu,Shousong Zhao,Jiangyang Lu,Junsheng Ji,Jingmin ZhaoPubMed 32427582
- Oncology reports 43:1319-13302020Cutaneous neurofibroma cells with active YAP promotes proliferation of macrophages resulting in increased accumulation of macrophages by modulating CCL5 and TGF‑β1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJing Jia et. al.PubMed 32323813
- Frontiers in immunology 9:17892018Extracellular Vesicles Work as a Functional Inflammatory Mediator Between Vascular Endothelial Cells and Immune Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBaharak Hosseinkhani et. al.PubMed 30131806
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.94 - 60 pg/mL
- Assay time
- 1h 30m
- Sensitivity
- = 0.091 pg/mL
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application sELISA | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed:1380064, PubMed:15923218, PubMed:16791620, PubMed:8525373, PubMed:9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed:23979485).
Alternative names
D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228
What's included?
Recommended products
Human RANTES ELISA Kit (CCL5) is a single-wash 90-min Simplestep used to quantify Human RANTES (CCL5) with a sensitivity of 0.091 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.
- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations
Key facts
- Detection method
- Colorimetric
- Sample types
- Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma
- Assay type
- Sandwich (quantitative)
- Reactive species
- Human
- Range
- 0.94 - 60 pg/mL
- Assay time
- 1h 30m
- Assay Platform
- Microplate
- Sensitivity
- = 0.091 pg/mL
Precision
Intra assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 8 | mean - | SD - | C.V. 5 |
Inter assay
| Sample | n | mean | SD | C.V. |
|---|---|---|---|---|
Sample Serum | n 3 | mean - | SD - | C.V. 2.8 |
Recovery
Sample specific recovery
| Sample type | Average % | Range |
|---|---|---|
Sample type Cell culture supernatant | Average % = 104 | Range 95 - 114 % |
Sample type Serum | Average % = 107 | Range 77 - 132 % |
Sample type EDTA Plasma | Average % = 105 | Range 81 - 119 % |
Sample type Heparin Plasma | Average % = 91 | Range 79 - 100 % |
Sample type Citrate plasma | Average % = 105 | Range 82 - 124 % |
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- +4°C
Notes
Human RANTES ELISA kit (CCL5) (ab174446) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of RANTES protein in human plasma, serum and cell culture supernatant. It uses our proprietary SimpleStep ELISA® technology. Quantitate human RANTES with 0.091 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
-Single-wash protocol reduces assay time to 90 minutes or less
-High sensitivity, specificity and reproducibility from superior antibodies
-Fully validated in biological samples
-96-wells plate breakable into 12 x 8 wells strips
A 384-well SimpleStep ELISA® microplate (Pre-coated 384 well Microplate SimpleStep ELISA® ab203359) is available to use as an alternative to the 96-well microplate provided with SimpeStep ELISA® kits.
RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) is a chemotactic cytokine for T cells, eosinophils, and basophils. RANTES also acts as a recruitment signal for leukocytes into inflammatory sites with the help of cyotokines (IL-2 and IFN-γ) released by CD8+ T cells. RANTES also activates and induces proliferation of natural-killer cells to form CHAK (CC-Chemokine-activated killer) cells. RANTES has also been identified to inhibit infection of HIV.
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6 product images
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (ab174446)
Human RANTES standard curve comparison.
Standard Curve comparison between human RANTES SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (ab174446)
Example of human RANTES standard curve in Sample Diluent NS.
The RANTES standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (ab174446)
Interpolated concentrations of native RANTES in human serum, and plasma samples.
The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 2.5%, plasma (citrate) 0.63%, plasma (heparin) 2.5% and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,141 pg/mL in neat serum, 13,050 pg/mL in plasma (citrate) and 3,157 pg/mL in plasma (heparin) and 4,457 pg/mL plasma (EDTA).
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (ab174446)
Interpolated concentrations of native RANTES in human PBMC cell culture supernatant stimulated with 1.5% PHA-M for 36 hours.
The supernatant was decanted and assayed. The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows: PBMC supernatant 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,156 pg/mL in neat PBMC supernatant.
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (ab174446)
Serum from ten individual healthy human male donors was measured in duplicate.
Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 977 pg/mL with a range of 135–2,378 pg/mL.
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (ab174446)
Comparison of PHA-M stimulated versus unstimulated PBMC supernatant.
PBMCs were stimulated with 1.5% PHA-M for 36 hours. The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,156 pg/mL in neat PBMC stimulated supernatant and non-detectable in unstimulated supernatant.
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