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AB174446

Human RANTES ELISA Kit (CCL5)

3

(3 Reviews)

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(17 Publications)

Human RANTES ELISA Kit (CCL5) is a single-wash 90-min Simplestep used to quantify Human RANTES (CCL5) with a sensitivity of 0.091 pg/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

- Colorimetric Sandwich ELISA - 450 nm readout : works on any standard plate reader
- Design your own immunoassay: we also offer the conjugation-ready antibody pair
- Cited in over 15 citations

View Alternative Names

D17S136E, SCYA5, CCL5, C-C motif chemokine 5, EoCP, Eosinophil chemotactic cytokine, SIS-delta, Small-inducible cytokine A5, T cell-specific protein P228, T-cell-specific protein RANTES, TCP228

7 Images
ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • ELISA

Supplier Data

ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • sELISA

Unknown

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)

Comparison of PHA-M stimulated versus unstimulated PBMC supernatant.

PBMCs were stimulated with 1.5% PHA-M for 36 hours. The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,156 pg/mL in neat PBMC stimulated supernatant and non-detectable in unstimulated supernatant.

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • sELISA

Unknown

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)

Interpolated concentrations of native RANTES in human serum, and plasma samples.

The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows : serum 2.5%, plasma (citrate) 0.63%, plasma (heparin) 2.5% and plasma (EDTA) 2.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,141 pg/mL in neat serum, 13,050 pg/mL in plasma (citrate) and 3,157 pg/mL in plasma (heparin) and 4,457 pg/mL plasma (EDTA).

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • sELISA

Unknown

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)

Serum from ten individual healthy human male donors was measured in duplicate.

Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 977 pg/mL with a range of 135–2,378 pg/mL.

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • sELISA

Unknown

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)

Interpolated concentrations of native RANTES in human PBMC cell culture supernatant stimulated with 1.5% PHA-M for 36 hours.

The supernatant was decanted and assayed. The concentrations of RANTES were measured in duplicates, interpolated from the RANTES standard curves and corrected for sample dilution. Undiluted samples are as follows : PBMC supernatant 1.25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean RANTES concentration was determined to be 2,156 pg/mL in neat PBMC supernatant.

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • sELISA

Unknown

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)

Human RANTES standard curve comparison.

Standard Curve comparison between human RANTES SimpleStep ELISA kit and traditional ELISA kit from leading competitor. SimpleStep ELISA kit shows increased sensitivity.

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)
  • sELISA

Unknown

Sandwich ELISA - Human RANTES ELISA Kit (CCL5) (AB174446)

Example of human RANTES standard curve in Sample Diluent NS.

The RANTES standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Plasma, Heparin Plasma, Citrate plasma, Cell culture supernatant, Serum, EDTA Plasma

Reacts with

Human

Assay type

Sandwich

Results type

Quantitative

Sensitivity

= 0.091 pg/mL

Range

0.94 - 60 pg/mL

Assay time

1h 30m

Assay Platform

Microplate

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Human RANTES ELISA Kit (CCL5) ab174446 is a rapid single-wash 90-min Sandwich ELISA to measure Human RANTES (CCL5)in cell culture supernatant, citrate plasma, EDTA plasma, heparin plasma, plasma, serum. This SimpleStep sensitivity is 0.091 pg/mL.

How the assay works

Human RANTES (CCL5)SimpleStep ELISA®employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details.

Assay Specificity

Our SimpleStep ELISA® kits use recombinant monoclonal antibodies rigorously validated to ensure the highest level of consistency and reproducibility, improved sensitivity and specificity and ease of scalability and security of supply.
Please refer to our protocol booklet for more details.

Human RANTES ELISA Kit (CCL5) ab174446 protocol summary

1. Mix: add samples/standards to the wells together with the capture and detector antibody cocktail. Incubate 1 hr at room temperature
2. Wash
3. Add TMB development solution - incubate for 10 min
4. Add Stop solution
5. Read the results on a plate reader at 450 nm

Design your own immunoassay

We offer the antibody pair used in this kit in a BSA and Azide-free format, ready for conjugation:

- Anti-RANTES antibody [EPR20534-97] - BSA and Azide free (Capture) ab244884
- Anti-RANTES antibody [EPR20534-199] - BSA and Azide free (Detector) ab244884

RANTES (Regulated on Activation, Normal T cell Expressed and Secreted) is a chemotactic cytokine for T cells, eosinophils, and basophils. RANTES also acts as a recruitment signal for leukocytes into inflammatory sites with the help of cyotokines (IL-2 and IFN-γ) released by CD8+ T cells. RANTES also activates and induces proliferation of natural-killer cells to form CHAK (CC-Chemokine-activated killer) cells. RANTES has also been identified to inhibit infection of HIV.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Serum", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "2.8" }, { "reproducibilityType": "Intra", "sample": "Serum", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "5" } ]
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Recovery

[ { "sample": "Cell culture supernatant", "range": "95 - 114 %", "average": "= 104" }, { "sample": "Serum", "range": "77 - 132 %", "average": "= 107" }, { "sample": "EDTA Plasma", "range": "81 - 119 %", "average": "= 105" }, { "sample": "Heparin Plasma", "range": "79 - 100 %", "average": "= 91" }, { "sample": "Citrate plasma", "range": "82 - 124 %", "average": "= 105" } ]
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What's included?

{ "values": { "1x96Tests": { "sellingSize": "1 x 96 Tests", "publicAssetCode":"ab174446-1x96Tests", "assetComponentDetails": [ { "size":"1 x 12 mL", "name":"Stop Solution", "number":"AB174446-CMP04", "productcode":"" }, { "size":"2 x 1 Vial", "name":"Human RANTES Lyophilized Recombinant Protein", "number":"AB174446-CMP06", "productcode":"" }, { "size":"1 x 20 mL", "name":"10X Wash Buffer PT (ab206977)", "number":"AB174446-CMP10", "productcode":"" }, { "size":"1 x 6 mL", "name":"Antibody Diluent CPR2", "number":"AB174446-CMP11", "productcode":"" }, { "size":"1 x 50 mL", "name":"Sample Diluent NS (ab193972)", "number":"AB174446-CMP02", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RANTES Capture Antibody", "number":"AB174446-CMP07", "productcode":"" }, { "size":"1 x 1 Unit", "name":"Plate Seals", "number":"AB174446-CMP09", "productcode":"" }, { "size":"1 x 12 mL", "name":"TMB Development Solution", "number":"AB174446-CMP05", "productcode":"" }, { "size":"1 x 1 Unit", "name":"SimpleStep Pre-Coated 96-Well Microplate (ab206978)", "number":"AB174446-CMP03", "productcode":"" }, { "size":"1 x 600 µL", "name":"10X Human RANTES Detector Antibody", "number":"AB174446-CMP08", "productcode":"" } ] } } }
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
+4°C
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Product protocols

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Target data

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) (PubMed : 1380064, PubMed : 15923218, PubMed : 16791620, PubMed : 8525373, PubMed : 9516414). May also be an agonist of the G protein-coupled receptor GPR75, stimulating inositol trisphosphate production and calcium mobilization through its activation. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells (PubMed : 23979485).
See full target information CCL5
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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 54: PubMed39364750

2024

Downregulation of chemokine (C‑C motif) ligand 5 induced by a novel 8‑hydroxyquinoline derivative (91b1) suppresses tumor invasiveness in esophageal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Cheuk-On Tang,Dessy Chan,Po-Yee Chung,Yijiang Liu,Alfred King-Yin Lam,Simon Law,Wolin Huang,Albert Sun-Chi Chan,Kim-Hung Lam,Yuanyuan Zhou

Journal of personalized medicine 14: PubMed39063997

2024

The Role of Pro-Inflammatory Chemokines CCL-1, 2, 4, and 5 in the Etiopathogenesis of Type 2 Diabetes Mellitus in Subjects from the Asir Region of Saudi Arabia: Correlation with Different Degrees of Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammad Muzaffar Mir,Jaber Alfaifi,Shahzada Khalid Sohail,Syeda Fatima Rizvi,Md Tanwir Akhtar,Mushabab Ayed Abdullah Alghamdi,Rashid Mir,Javed Iqbal Wani,Zia Ul Sabah,Fahad A Alhumaydhi,Fahad Alremthi,AbdulElah Al Jarallah AlQahtani,Muffarah Hamid Alharthi,Masoud Ishag Elkhalifa Adam,Imadeldin Elfaki,Hany M A Sonpol

Molecular carcinogenesis 63:1827-1841 PubMed39016636

2024

LAIR1 promotes hepatocellular carcinoma cell metastasis and induces M2-macrophage infiltration through activating AKT-IKKβ-p65 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Banglun Pan,Shuling Shen,Jun Zhao,Zhu Zhang,Dongjie Ye,Xiaoxia Zhang,Yuxin Yao,Yue Luo,Xiaoqian Wang,Nanhong Tang

Nature communications 15:2107 PubMed38453911

2024

Single-cell profiling identifies IL1B macrophages associated with inflammation in PD-1 inhibitor-induced inflammatory arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyue Zhou,Xiaoxiang Zhou,Xu Jiang,Bo Yang,Xin Lu,Yunyun Fei,Lidan Zhao,Hua Chen,Li Zhang,Xiaoyan Si,Naixin Liang,Yadong Wang,Dan Yang,Yezi Peng,Yiying Yang,Zhuoran Yao,Yangzhige He,Xunyao Wu,Wen Zhang,Min Wang,Huaxia Yang,Xuan Zhang

Nature communications 14:3364 PubMed37291128

2023

Transcriptional control of pancreatic cancer immunosuppression by metabolic enzyme CD73 in a tumor-autonomous and -autocrine manner.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Tang,Xing Huang,Minghao Lu,Gang Zhang,Xu Han,Tingbo Liang

Oncology letters 25:225 PubMed37153045

2023

Study on the mechanism of oral administration of tetrandrine during neoadjuvant chemotherapy for colon cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Junmei Li,Feng Yu,Bo Wang,Bing Liu

Research (Washington, D.C.) 6:0033 PubMed37040518

2023

PTBP2-Mediated Alternative Splicing of IRF9 Controls Tumor-Associated Monocyte/Macrophage Chemotaxis and Repolarization in Neuroblastoma Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jue Tang,Jing He,Huiqin Guo,Huiran Lin,Meng Li,Tianyou Yang,Hai-Yun Wang,Di Li,Jiabin Liu,Le Li,Huimin Xia,Zhenjian Zhuo,Lei Miao

Nature communications 13:2692 PubMed35577786

2022

Hyperphosphorylated tau self-assembles into amorphous aggregates eliciting TLR4-dependent responses.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan X Meng,Yu Zhang,Dominik Saman,Arshad M Haider,Suman De,Jason C Sang,Karen Brown,Kun Jiang,Jane Humphrey,Linda Julian,Eric Hidari,Steven F Lee,Gabriel Balmus,R Andres Floto,Clare E Bryant,Justin L P Benesch,Yu Ye,David Klenerman

Oncology letters 22:696 PubMed34457051

2021

Cancer-associated fibroblast-derived CCL5 contributes to cisplatin resistance in A549 NSCLC cells partially through upregulation of lncRNA HOTAIR expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangjun Sun,Zhijie Chen

Molecular cancer therapeutics 19:2598-2611 PubMed32999046

2020

Anti-S100A4 Antibody Therapy Is Efficient in Treating Aggressive Prostate Cancer and Reversing Immunosuppression: Serum and Biopsy as a Clinical Predictor.

Applications

Unspecified application

Species

Unspecified reactive species

Arsheed A Ganaie,Adrian P Mansini,Tabish Hussain,Arpit Rao,Hifzur R Siddique,Ashraf Shabaneh,Marina G Ferrari,Paari Murugan,Jörg Klingelhöfer,Jinhua Wang,Noona Ambartsumian,Christopher A Warlick,Badrinath R Konety,Mohammad Saleem
View all publications
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