AB324510

Human/Rat Tyrosine Hydroxylase ELISA Kit

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Human/Rat Tyrosine Hydroxylase ELISA Kit is a Sandwich (quantitative) ELISA for the measurement of Human/Rat Tyrosine Hydroxylase in Human, Rat in Cell/Tissue Extracts samples.

View Alternative Names

TYH, TH, Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase

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Sandwich ELISA - Human/Rat Tyrosine Hydroxylase ELISA Kit (AB324510)

sELISA

Lab

Sandwich ELISA - Human/Rat Tyrosine Hydroxylase ELISA Kit (AB324510)

Interpolated concentration of native Tyrosine Hydroxylase was measured in duplicate at different sample concentrations in 96-well vs. 384-well plates. Undiluted samples are 500 ng/mL PC-12 cell extract. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW + Enhancer.

sELISA

Lab

Sandwich ELISA - Human/Rat Tyrosine Hydroxylase ELISA Kit (AB324510)

Interpolated concentration of native Tyrosine Hydroxylase was measured in duplicate at different sample concentrations. Undiluted samples are as follows : PC-12 cell extract 500 ng/mL, and rat brain stem tissue extract 194 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1X Cell Extraction Buffer SSW + Enhancer.

sELISA

Lab

Sandwich ELISA - Human/Rat Tyrosine Hydroxylase ELISA Kit (AB324510)

Example of human/rat Tyrosine Hydroxylase standard curve in 1X Cell Extraction Buffer SSW + Enhancer. Background-subtracted data values (mean +/- SD) are graphed.

sELISA

Lab

Sandwich ELISA - Human/Rat Tyrosine Hydroxylase ELISA Kit (AB324510)

Example of human/rat Tyrosine Hydroxylase standard curve in 1X Cell Extraction Buffer SSW + Enhancer in 96-well vs. 384-well plate. Background-subtracted data values (mean +/- SD) are graphed.

Key facts

Detection method

Colorimetric

Sample types

Cell Extract, Tissue Extracts

Reacts with

Human, Rat

Assay type

Sandwich (quantitative)

Sensitivity

= 0.154 ng/mL

Range

0.75 - 48 ng/mL

Assay time

1h 30m

Assay Platform

Pre-coated microplate (12 x 8 well strips)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }

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Product details

Human/Rat Tyrosine Hydroxylase ELISA Kit is a single-wash 90-min Simplestep used to quantify Human/Rat Tyrosine Hydroxylase with a sensitivity of 0.154ng/ml. The assay uses a simple mix-wash-read protocol with just one incubation and one wash step.

-Colormetric sandwich ELISA - 450 nm readout- works on any standard plate reader.

-Different formats for different needs: 10 x 96 plates for bulk orders and 384-well for higher throughput.

-Design your own immunoassay: we also offer the conjugation-ready antibody pair.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Precision

[ { "reproducibilityType": "Inter", "sample": "Extract", "replicates": 3, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 8.8" }, { "reproducibilityType": "Intra", "sample": "Extract", "replicates": 8, "mean": null, "standardDeviation": null, "coefficientOfVariability": "= 4.7" } ]

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Recovery

[ { "sample": "Cell Lysate", "range": "83 - 104 %", "average": "= 94" }, { "sample": "Tissue Extracts", "range": "95 - 100 %", "average": "= 97" } ]

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What's included?

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Properties and storage information

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

+4°C

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Product protocols

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Target data

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (PubMed : 15287903, PubMed : 1680128, PubMed : 17391063, PubMed : 24753243, PubMed : 34922205, PubMed : 8528210, Ref.18). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan with lower specificity (By similarity). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).. Isoform 5. Lacks catalytic activity.. Isoform 6. Lacks catalytic activity.

See full target information TH

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Product promise

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